Microorganism combination and degradation method for degrading heavy metal-enriched plants
A technology for enriching plants and heavy metals, applied in the field of microorganisms, can solve the problems of complex pollution remediation, high cost, secondary pollution, etc.
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Embodiment 1
[0050] A microbial combination for degrading uranium-enriched ryegrass, which is Bacillus subtilis-white rot fungus (P.chrysosporium)-Trichoderma reesei (T.ressei)-strong promoter strain QD-1 (Pterula sp. strainQD-1), wherein Bacillus subtilis: white rot fungus: Trichoderma reesei: strong promoter strain=0.5:0.5:1.5:1.5.
[0051] The bacterial concentration of the microbial combination is OD600=0.8, pH7.0.
[0052] White rot fungus, Trichoderma reesei and QD-1 were cultured separately in PDA medium, and the bacteria were cultured in bacterial culture medium; they were placed in a constant temperature shaker at 37°C at 150rpm for 48 hours, and then the bacterial solution was taken separately for compounding; Microbial combination Use a homogenizer to break up the bacterial balls, and then adjust the bacterial concentration with sterile PDA medium. PDA medium: 200g of potatoes, 20g of glucose, 20g of agar, 1L of distilled water; the pH of the medium is 6.8.
[0053] Peel the p...
Embodiment 2
[0061] Other contents are as in Example 1, a microbial combination for degrading uranium-enriched ryegrass, which is Bacillus subtilis-white rot fungus (P.chrysosporium)-Trichoderma reesei (T.ressei)-strong promoter strain QD-1 (Pterula sp.strain QD-1), wherein white rot fungus: Trichoderma reesei: QD-1 = 1.5: 1.5: 0.5: 0.5.
[0062] The bacterial concentration of the microbial combination is OD600=0.8, pH7.0.
[0063] White rot fungus, Trichoderma reesei and QD-1 were cultured separately with PDA medium, and the bacteria were cultured with bacterial culture medium; they were placed in a constant temperature shaker at 37°C at 150rpm for 48 hours, and then the bacterial solution was taken separately for compounding; Microbial combination Use a homogenizer to break up the bacterial balls, and then adjust the bacterial concentration with sterile PDA medium. PDA medium: 200g of potatoes, 20g of glucose, 20g of agar, 1L of distilled water; the pH of the medium is 7.2.
[0064] Ba...
Embodiment 3
[0066] Other contents are as in Example 1, a microbial combination for degrading uranium-enriched ryegrass, Bacillus subtilis: white rot fungus: Trichoderma reesei: strong promoter strain = 0.8:0.8:1.2:1.2.
[0067] The bacterial concentration of the microbial combination is OD600=0.8, pH7.0.
[0068] White rot fungus, Trichoderma reesei and QD-1 were cultured separately with PDA medium, and the bacteria were cultured with bacterial culture medium; they were placed in a constant temperature shaker at 37°C at 150rpm for 48 hours, and then the bacterial solution was taken separately for compounding; The microbial combination uses a homogenizer to disperse the bacterium balls, and then uses sterile PDA medium to adjust the bacterial concentration. PDA medium: 200 g of potatoes, 20 g of glucose, 20 g of agar, 1 L of distilled water; the pH of the medium is 7.
[0069] Bacterial medium is LB medium: yeast powder 5g, peptone 10g, NaCl 10g, agar 20g, distilled water 1 L, pH7.2.
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