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Microorganism combination and degradation method for degrading heavy metal-enriched plants

A technology for enriching plants and heavy metals, applied in the field of microorganisms, can solve the problems of complex pollution remediation, high cost, secondary pollution, etc.

Inactive Publication Date: 2018-12-21
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, most of the uranium-contaminated areas are polluted by compound heavy metals, which makes the pollution remediation extremely complicated
[0005] Although conventional physical and chemical methods can be used to treat heavy metals in water-soil media, they are not easy to operate in the field because of their high cost, easy to cause secondary pollution, and great harm to the human body during the treatment process; more advantages
So far, research on bioremediation of heavy metal-contaminated environments is mostly limited to laboratories, and only a few experiments have entered the field stage
[0006] Phytoremediation technology is currently a research hotspot in the field of remediation of heavy metal pollution, but there is a relative lack of research on phytoremediation of heavy metals after remediation.
[0007] However, the use of enriched plants to remediate heavy metal pollution will produce a large amount of heavy metal-enriched biomass, which will cause secondary pollution if not properly disposed of.

Method used

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  • Microorganism combination and degradation method for degrading heavy metal-enriched plants
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  • Microorganism combination and degradation method for degrading heavy metal-enriched plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A microbial combination for degrading uranium-enriched ryegrass, which is Bacillus subtilis-white rot fungus (P.chrysosporium)-Trichoderma reesei (T.ressei)-strong promoter strain QD-1 (Pterula sp. strainQD-1), wherein Bacillus subtilis: white rot fungus: Trichoderma reesei: strong promoter strain=0.5:0.5:1.5:1.5.

[0051] The bacterial concentration of the microbial combination is OD600=0.8, pH7.0.

[0052] White rot fungus, Trichoderma reesei and QD-1 were cultured separately in PDA medium, and the bacteria were cultured in bacterial culture medium; they were placed in a constant temperature shaker at 37°C at 150rpm for 48 hours, and then the bacterial solution was taken separately for compounding; Microbial combination Use a homogenizer to break up the bacterial balls, and then adjust the bacterial concentration with sterile PDA medium. PDA medium: 200g of potatoes, 20g of glucose, 20g of agar, 1L of distilled water; the pH of the medium is 6.8.

[0053] Peel the p...

Embodiment 2

[0061] Other contents are as in Example 1, a microbial combination for degrading uranium-enriched ryegrass, which is Bacillus subtilis-white rot fungus (P.chrysosporium)-Trichoderma reesei (T.ressei)-strong promoter strain QD-1 (Pterula sp.strain QD-1), wherein white rot fungus: Trichoderma reesei: QD-1 = 1.5: 1.5: 0.5: 0.5.

[0062] The bacterial concentration of the microbial combination is OD600=0.8, pH7.0.

[0063] White rot fungus, Trichoderma reesei and QD-1 were cultured separately with PDA medium, and the bacteria were cultured with bacterial culture medium; they were placed in a constant temperature shaker at 37°C at 150rpm for 48 hours, and then the bacterial solution was taken separately for compounding; Microbial combination Use a homogenizer to break up the bacterial balls, and then adjust the bacterial concentration with sterile PDA medium. PDA medium: 200g of potatoes, 20g of glucose, 20g of agar, 1L of distilled water; the pH of the medium is 7.2.

[0064] Ba...

Embodiment 3

[0066] Other contents are as in Example 1, a microbial combination for degrading uranium-enriched ryegrass, Bacillus subtilis: white rot fungus: Trichoderma reesei: strong promoter strain = 0.8:0.8:1.2:1.2.

[0067] The bacterial concentration of the microbial combination is OD600=0.8, pH7.0.

[0068] White rot fungus, Trichoderma reesei and QD-1 were cultured separately with PDA medium, and the bacteria were cultured with bacterial culture medium; they were placed in a constant temperature shaker at 37°C at 150rpm for 48 hours, and then the bacterial solution was taken separately for compounding; The microbial combination uses a homogenizer to disperse the bacterium balls, and then uses sterile PDA medium to adjust the bacterial concentration. PDA medium: 200 g of potatoes, 20 g of glucose, 20 g of agar, 1 L of distilled water; the pH of the medium is 7.

[0069] Bacterial medium is LB medium: yeast powder 5g, peptone 10g, NaCl 10g, agar 20g, distilled water 1 L, pH7.2.

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Abstract

The invention belongs to the field of microorganism, in particular to a microorganism combination and a degradation method for degrading heavy metal-enriched plants. The microorganism combination consists of B.subtilis, P.chrysosporium, T.ressei and Pterulasp.strain QD-1 accoriding to a ratio of (0.5 to 1.5):(0.5 to 1.5):(0.5 to 1.5):(0.5 to 1.5):(0.5 to 1.5). The microorganism combination has theadvantages that the microorganism degrading and capacity reduction with low cost, low energy consumption and green and safe effects are realized; the good effect is realized on the degrading effect of enriched plant and the extracting of heavy metals; the lignocellulose is highly and efficiently degraded so as to reach the high-efficiency degrading and high-flux capacity reduction of biomass; thebacterial strain has good uranium tolerance, and the antagonism is avoided between the bacterial strains.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a combination of microorganisms and a degradation method for degrading heavy metal-rich plants. Background technique [0002] Heavy metal materials have always been an indispensable resource in the development of human industrial technology. With the formation of more and more high-tech industries today, although a variety of heavy metal substitute materials have been produced, the mining volume and demand of heavy metal resources have not decreased. On the contrary, human mining of heavy metal mineral resources has greatly increased. In the process of utilization of heavy metal resources, including mining, tailings stacking, ore refining and subsequent processing and utilization, local heavy metal concentrations will be too high, which will affect the surrounding environment, and the polluted area will gradually expand with rainwater and watersheds. [0003] The main s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14C12N13/00C02F3/34B09C1/10C12R1/125C12R1/645C12R1/885C02F101/20
CPCB09C1/10B09C1/105C02F3/34C02F3/347C02F2101/20C12N1/14C12N1/20C12N13/00
Inventor 陈晓明罗学刚王丹张详辉
Owner SOUTHWEAT UNIV OF SCI & TECH
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