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PEG type thermosensitive hydrogel three-dimensional cell scaffold as well as preparation method and application thereof

A temperature-sensitive hydrogel and three-dimensional cell technology, applied in the field of PEG-based temperature-sensitive hydrogel three-dimensional cell scaffolds and their preparation, can solve the problems of restricting the application of hydrogel scaffolds, severe syneresis, refractory degradation, etc., and achieve good temperature sensitivity , the degree of syneresis is small, and the preparation method is simple and feasible

Inactive Publication Date: 2018-12-21
NANHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is prone to phase separation under physiological temperature conditions, and the phenomenon of syneresis is serious, and it has certain toxicity and refractory properties, which limit its application in hydrogel scaffolds.
However, there are no literature reports on the use of such PEG-like thermosensitive hydrogels as three-dimensional cell scaffolds

Method used

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  • PEG type thermosensitive hydrogel three-dimensional cell scaffold as well as preparation method and application thereof
  • PEG type thermosensitive hydrogel three-dimensional cell scaffold as well as preparation method and application thereof
  • PEG type thermosensitive hydrogel three-dimensional cell scaffold as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0031] This embodiment provides a preparation method and application of a PEG-based thermosensitive hydrogel three-dimensional cell scaffold, which includes the following steps:

[0032] (1) Weigh 2.2371g of 2-methyl-2-acrylic acid-2-(2-methoxyethoxy)ethyl ester, 0.5462g of oligoethylene glycol methyl ether methacrylate, N,N'- Put 0.0431g of methylenebisacrylamide and 0.0284g of sodium lauryl sulfate in a 250mL three-necked round bottom flask, add 100mL of distilled water to dissolve, and continuously blow nitrogen to remove oxygen. After 1h, raise the temperature to 70°C, dissolve 0.0810g of initiator potassium persulfate in 5mL of distilled water, add it to the above solution, keep the nitrogen atmosphere, and continue the reaction for 5h to obtain PEG-based thermosensitive nanogel;

[0033] (2) The PEG-based thermosensitive nanogel obtained in step (1) was dialyzed for 7 days, and the water was changed 3 times a day to remove residual reactants, electrolytes and by-products...

Embodiment 2

[0043] This embodiment provides a preparation method and application of a PEG-based thermosensitive hydrogel three-dimensional cell scaffold, which includes the following steps:

[0044] (1) Weigh 2.3664g of 2-methyl-2-acrylic acid-2-(2-methoxyethoxy)ethyl ester, 0.3368g of oligoethylene glycol methyl ether methacrylate, N,N'- Put 0.0431g of methylenebisacrylamide and 0.0284g of sodium lauryl sulfate in a 250mL three-necked round bottom flask, add 100mL of distilled water to dissolve, and continuously blow nitrogen to remove oxygen. After 1h, raise the temperature to 70°C, dissolve 0.0810g of initiator potassium persulfate in 5mL of distilled water, add it to the above solution, keep the nitrogen atmosphere, and continue the reaction for 5h to obtain PEG-based thermosensitive nanogel;

[0045] (2) The PEG-based thermosensitive nanogel obtained in step (1) was dialyzed for 7 days, and the water was changed 3 times a day to remove residual reactants, electrolytes and by-products...

Embodiment 3

[0047] This embodiment provides a method for preparing a PEG-based thermosensitive hydrogel three-dimensional cell scaffold, which includes the following steps:

[0048] (1) Weigh 2.5004g of 2-methyl-2-acrylic acid-2-(2-methoxyethoxy)ethyl ester, 0.1260g of oligoethylene glycol methyl ether methacrylate, N,N'- Put 0.0431g of methylenebisacrylamide and 0.0284g of sodium lauryl sulfate in a 250mL three-necked round bottom flask, add 100mL of distilled water to dissolve, and continuously blow nitrogen to remove oxygen. After 1h, raise the temperature to 70°C, dissolve 0.0810g of initiator potassium persulfate in 5mL of distilled water, add it to the above solution, keep the nitrogen atmosphere, and continue the reaction for 5h to obtain PEG-based thermosensitive nanogel;

[0049] (2) The PEG-based thermosensitive nanogel obtained in step (1) was dialyzed for 7 days, and the water was changed 3 times a day to remove residual reactants, electrolytes and by-products. After purifica...

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Abstract

The invention relates to a PEG type thermosensitive hydrogel three-dimensional cell scaffold and as well as a preparation method and application thereof. The preparation method of the three-dimensional cell scaffold comprises the steps of mixing acrylate water-soluble macromolecular polymerizable monomer containing a PEG side chain, an initiator, a surfactant, a cross-linking agent and / or comonomer, reacting and obtaining PEG type thermosensitive nano gel; mixing the PEG type thermosensitive nano gel with cells uniformly at room temperature, and forming the PEG type thermosensitive hydrogel three-dimensional cell scaffold by thermally inducing in-situ embedded cells. The preparation method is easy to conduct, is featured by mild conditions, and damages cells slightly. Compared with a traditional PNIPAM type thermosensitive hydrogel scaffold, the PEG type thermosensitive hydrogel scaffold has high biocompatibility, is degradable, is adjustable in nature, prolongs syneresis slightly along with time, and can keep cells growing and proliferating for long time, thereby being an ideal tissue engineering material, and being used in fields like in vitro culture of cells, tissue repair andtumor treatment.

Description

technical field [0001] The invention relates to the field of biomedical polymer materials, in particular to a PEG-based temperature-sensitive hydrogel three-dimensional cell scaffold and a preparation method and application thereof. Background technique [0002] Hydrogel is a three-dimensional space network structure formed by colloidal particles or polymer chains through physical or chemical cross-linking, which has similar physiological and chemical properties to living tissues, such as high water content, low surface tension and good Biocompatibility, etc., are widely used in biomedical fields such as cell scaffolds, tissue repair, and drug controlled release. [0003] Thermosensitive hydrogels are a class of functional hydrogels that can respond rapidly to ambient temperature and produce reversible changes. It is a flowable liquid in a relatively low temperature environment, which can be uniformly mixed with various seed cells, drugs, and active molecules, and gels rapi...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L51/10C08F290/06C08F220/28C08F222/38A61L27/18A61L27/52A61L27/58C12N5/00
CPCA61L27/18A61L27/52A61L27/58A61L2400/06C08F290/062C08J3/075C08J2351/10C12N5/0062C12N2513/00C12N2533/30C08L51/08C08F220/285
Inventor 刘阳杨雯霏张佩张偲瑶李蓉贺艳
Owner NANHUA UNIV
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