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Construction of Lactobacillus crispatus BT1386 and application in treatment of bacterial vaginosis

A technology of Lactobacillus crispatus and puc57-cxcl12, which can be used in antibacterial drugs, medical preparations containing active ingredients, sexual diseases, etc., and can solve problems such as inability to meet tissue repair

Inactive Publication Date: 2018-12-18
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, CXCL12 is easily degraded by dipeptide aminopeptidase IV (DPP IV) in human blood, and the endogenous CXCL12 level produced during the repair of severe tissue damage cannot meet the needs of tissue repair, so it is necessary to introduce exogenous CXCL12

Method used

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  • Construction of Lactobacillus crispatus BT1386 and application in treatment of bacterial vaginosis
  • Construction of Lactobacillus crispatus BT1386 and application in treatment of bacterial vaginosis
  • Construction of Lactobacillus crispatus BT1386 and application in treatment of bacterial vaginosis

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Detailed description of the embodiment: the drug effect is evaluated by a rat model of bacterial vaginosis

[0056] 1. Establishment of rat vaginitis model

[0057] (1) The experimental groups were negative control group, model group, metronidazole treatment, Lactococcus lactis engineering strain treatment group, and Lactobacillus crispatus engineering strain treatment group, with 5 animals in each group;

[0058] (2) Antibiotic treatment, cut the medical absorbent gelatin sponge into a size of 0.5cm×0.5cm, absorb 50 μL of antibiotics with a concentration of 100mg / mL; after the gelatin is fully absorbed and saturated with antibiotics, insert the gelatin sponge containing antibiotics into the vaginal plug and intrauterine cavity; antibiotics were treated for 6 times, and the treatment was continued for three days and recovered once;

[0059] (3) After antibiotic treatment, the B-hemolytic streptococcus, Staphylococcus aureus, and Escherichia coli were prepared into a mi...

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Abstract

The invention discloses construction of Lactobacillus crispii BT1386 and application in treatment of bacterial vaginosis. First, human and mouse chemokine CXCL12 also known as stromal cell-derived factor-1 (SDF-1) is chemically synthesized, and the human and mouse chemokine CXCL12 belongs to is a small molecule protein in a CXC chemokine family. Secretory expression transmembrane peptide SPusp45 which is unique to Lactococcus lactis is added to construct a recombinant plasmid pMG36e-CXCL12, and the recombinant plasmid is then electrotransformed into the Lactobacillus crispii BT1386 to achievesecretory expression of the chemokine CXCL12. The chemokine CXCL12 is continuously expressed by vaginal embolization to achieve the remission and treatment of the bacterial vaginosis.

Description

technical field [0001] The invention relates to a preparation method of Lactobacillus crispatus BT1386 ​​secreting and expressing chemokine CXCL12 and its application in bacterial vaginosis, belonging to the field of biopharmaceuticals. Background technique [0002] Chemokines are a class of small-molecule polypeptide substances that have directional chemotaxis and recruitment effects on leukocytes and stem cells. Their structures and functions are similar, and most of them have four conserved cysteines in the amino acid sequence. Depending on the number and spacing of N-terminal cysteines, they are divided into C-, CXC-, CC- and CX3C-4 chemokines. Chemokine CXCL12, also known as stromal cell-derived factor-1 (SDF-1), has two forms, SDF-1α / CXCL12a and SDF-1β / CXCL12b. CXCR4 is a specific receptor for the chemokine CXCL12, which belongs to the G protein-coupled transmembrane receptor family and has a high affinity with CXCL12. It is widely expressed in blood, immune and centr...

Claims

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Application Information

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IPC IPC(8): C12N15/74G01N33/68G01N33/569A61K35/747A61P31/04A61P15/02
CPCA61K2035/115A61P15/02A61P31/04C12N15/74G01N33/56911G01N33/68
Inventor 陈廷涛田溥塬
Owner NANCHANG UNIV
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