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Tissue culture seedling breeding method of dragon fruits

A tissue culture, dragon fruit technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of difficulty in meeting market demands, obvious shape separation of offspring, large differences in germination, etc., and achieves good disinfection and sterilization effects. , the induction time is shortened, and the differentiation rate is high.

Inactive Publication Date: 2018-11-23
广西驰胜农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the breeding methods of seeds, cuttings and divisions are mostly used in the cultivation of dragon fruit. Because of the large difference in germination and the obvious separation of offspring shapes, the seed breeding of pitayas is difficult to meet the market demand; in order to solve the above problems, Adopting the method of tissue culture to cultivate dragon fruit not only has a short cycle, but also has high seedling quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for raising dragon fruit tissue culture seedlings, comprising the following steps:

[0023] S1: Collect the young shoot tip tissue from a healthy and excellent mother plant, cut it into small sections with a length of 4cm in a sterile environment, rinse it with tap water, and carefully peel off the small spines and soft hairs on the spines After removing the pith, rinse it with sterile water, soak it in an alcohol solution with a volume concentration of 70% for 20 seconds, then disinfect it with 0.1% mercuric solution for 10 minutes, rinse it with sterile water for 5 times, and then rinse it with sterile water. Bacteria filter paper was used as explants after absorbing the surface moisture;

[0024] S2: Inoculate the explants described in step S1 into the induction medium and cultivate them for 35 days to obtain sterile buds, the induction medium is MS medium+0.08mg / L NAA+2mg / L 6-BA+0.5mg / L IAA +35g / L sucrose+5g / L agar, put the above substances into the cultur...

Embodiment 2

[0030] A method for raising dragon fruit tissue culture seedlings, comprising the following steps:

[0031] S1: Collect the young shoot tip tissue from the excellent mother plant that grows vigorously, cut it into small sections with a length of 4.5cm in an aseptic environment, rinse it with tap water, and carefully peel off the small spines and soft tissue on the thorn base. After removing the pith of the hair, rinse it with sterile water, soak it in an alcohol solution with a volume concentration of 72% for 25 seconds, then disinfect it with 0.1% mercury liter solution for 11 minutes, rinse it with sterile water for 5 times, and then use it again. After the sterile filter paper absorbs the surface moisture, it is an explant;

[0032] S2: Inoculate the explants described in step S1 into the induction medium and cultivate them for 38 days to obtain sterile buds, the induction medium is MS medium+0.1mg / L NAA+2.5mg / L 6-BA+0.6mg / L IAA+38g / L sucrose+6g / L agar, put the above-menti...

Embodiment 3

[0038] A method for raising dragon fruit tissue culture seedlings, comprising the following steps:

[0039] S1: Collect the tender shoot tip tissue from a healthy and excellent mother plant, cut it into small sections with a length of 5 cm in an aseptic environment, rinse it with tap water, and carefully peel off the small spines and soft hairs on the spines After removing the pith, rinse it with sterile water, soak it in an alcohol solution with a volume concentration of 75% for 30 seconds, then disinfect it with 0.1% mercuric solution for 12 minutes, rinse it with sterile water for 5 times, and then rinse it with sterile water. Bacteria filter paper was used as explants after absorbing the surface moisture;

[0040] S2: Inoculate the explants described in step S1 into the induction medium and cultivate them for 42 days to obtain sterile buds, the induction medium is MS medium+0.12mg / L NAA+3mg / L 6-BA+0.7mg / L IAA +40g / L sucrose+7g / L agar, put the above substances into the cul...

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Abstract

The invention provides a tissue culture seedling breeding method of dragon fruits, belonging to the field of seedling breeding of the dragon fruits. The method comprises the steps of firstly, collecting young and tender stem tip tissues from selected good stock plants, washing, then stripping the small thorns and soft hairs on areole and removing medulla; after that, sterilizing to obtain explants, and then culturing the explants in an induction medium to obtain sterile shoots; putting the sterile shoots into a seedling formation culture medium for performing continuous differentiation; finally, enabling the shoots to grow into seedlings in a rooting medium, domesticating and then carrying out subsequent breeding of the seedlings. The method provided by the invention can promote the cultivation speed of the seedlings of the dragon fruits, reduces the seedling breeding period, guarantees the quality of the seedlings, stabilizes the excellent traits of the genetic stock plants, and is suitable for large-scale systematic planting.

Description

【Technical field】 [0001] The invention relates to the technical field of pitaya seedling cultivation, in particular to a method for pitaya tissue culture seedling cultivation. 【Background technique】 [0002] Dragon fruit is rich in plant-based albumin, anthocyanin, vitamins and water-soluble dietary fiber. Regular consumption has the effects of losing weight, lowering cholesterol, preventing constipation, and colorectal cancer. The plant-based albumin contained in it can interact with heavy metal ions in the human body. Combined, excreted from the body by the excretory system, and play a role in detoxification. The anthocyanins contained in it can protect the human body from the damage of free radicals, enhance the elasticity of blood vessels, and help prevent many diseases related to free radicals. Therefore, the dragon fruit planting industry has broad market prospects and economic benefits in China. [0003] At present, the breeding methods of seeds, cuttings and divisi...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001A01H4/008
Inventor 谢宗宜
Owner 广西驰胜农业科技有限公司
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