Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biosurfactant fermentation production technology based on trace metal ion regulation

A biological surface, trace metal technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems such as the reduction of the ability of biosurfactants, and achieve the effects of increasing production, stimulating growth and metabolism

Active Publication Date: 2018-11-16
EAST CHINA UNIV OF SCI & TECH
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing technology only optimizes the trace elements in the culture medium, but does not fully consider the binding effect of biosurfactant micelles on trace element ions as the fermentation progresses, resulting in the reduction of the growth of production strains and the ability of fermentation to produce biosurfactants

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Fermentation medium: sucrose 20.0g / L, (NH 4 ) 2 SO 4 2.0g / L, KH 2 PO 4 6.0g / L, Na 2 HPO 4 12H 2 O6.0g / L, MgSO 4 0.3g / L, yeast powder 0.5g / L, CaCl 2 0.001g / L, CoCl 2 ·6H 2 O 0.018g / L, NiCl 2 ·6H 2 O 0.007g / L, CuCl 2 2H 2 O 0.002g / l, FeSO 4 ·7H 2 O 0.083g / L.

[0019] Biosurfactant producing bacteria: Bacillus subtilis TD-7 (ZL201010107949.3)

[0020] Inoculate Bacillus subtilis TD-7 into the above medium, the inoculum amount is 2wt%, the fermentation temperature is 37°C, the initial pH is 7.0, 12 hours after inoculation, the pH of the fermentation broth is 6.5, start the feeding system, and add a small amount of Element Fe 2+ Fe in the fermentation broth 2+ Concentration of 20mg / L to the end of fermentation, lipopeptide content in the fermentation broth is 4.1g / L. (the prior art fermented under the same conditions of initial medium composition, inoculum size, fermentation temperature and pH, but did not supplement Fe in the later stage 2+ , lipope...

Embodiment 2

[0022] Fermentation medium: sucrose 20.0g / L, (NH 4 ) 2 SO 4 2.0g / L, KH 2 PO 4 6.0g / L, Na 2 HPO 4 12H 2 O6.0g / L, MgSO 4 0.3g / L, yeast powder 0.5g / L, CaCl 2 0.001g / L, CoCl 2 ·6H 2 O 0.018g / L, NiCl 2 ·6H 2 O 0.007g / L, CuCl 2 2H 2 O 0.002g / l, FeSO 4 ·7H 2 O 0.083g / L.

[0023] Biosurfactant producing bacteria: Bacillus subtilis TD-7 (ZL201010107949.3)

[0024] Inoculate Bacillus subtilis TD-7 into the above medium, the inoculum amount is 2wt%, the fermentation temperature is 37°C, the initial pH is 7.0, 12 hours after inoculation, the pH of the fermentation broth is 6.7, start the feeding system, and add a small amount of Element Fe 2+ Fe in the fermentation broth 2+ Concentration of 40mg / L to the end of fermentation, lipopeptide content in the fermentation broth is 4.8g / L. (the prior art fermented under the same conditions of initial medium composition, inoculum size, fermentation temperature and pH, but did not supplement Fe in the later stage 2+ , lipope...

Embodiment 3

[0026]Fermentation medium: glucose 24g / L, urea 6g / L, yeast powder 1g / L, potassium dihydrogen phosphate 6.0g / L1g / L, magnesium sulfate 2.4%g / L, calcium chloride 0.04g / L, chromium chloride 0.008g / L.

[0027] Biosurfactant producing bacteria: Pseudomonas aeruginosa FN2 (Liu J F, Wu G, Yang S Z, et al. Structural characterization of rhamnolipid produced by Pseudomonas aeruginosa strain FIN2 isolated from oil reservoir water. World Journal of Microbiology & Biotechnology, 2014, 30 5): 1473-84).

[0028] Inoculate Pseudomonas aeruginosa FN2 into the above-mentioned medium, the inoculum amount is 2wt%, the fermentation temperature is 35°C, the initial pH is 7.0, 8.2 hours after inoculation, the fermentation broth Ph=6.6, start the feeding system, feed Add trace element Fe 2+ Fe in the fermentation broth 2+ Concentration of 25mg / L to the end of fermentation, glycolipid content in the fermentation broth is 8.2g / L. (the prior art fermented under the same conditions of initial medium ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a biosurfactant fermentation production technology based on trace metal ion regulation. Trace elements Fe<2+> and Mn<2+> are supplemented in a biosurfactant production bacteria culture process. Compared with the prior art, the biosurfactant fermentation production technology is characterized in that the trace elements are duly supplemented into fermentation broth on the basis of the influence of trace elements to the growth and metabolism of production strains and the change characteristics of trace elements in the fermentation broth so as to improve the growth and metabolism activity of the production strains and improve production efficiency and the yield of the biosurfactant.

Description

technical field [0001] The invention relates to a biosurfactant fermentation production process, in particular to a biosurfactant fermentation production process based on trace metal ion regulation. Background technique [0002] Due to its high structural diversity, excellent surface activity, biodegradability, low toxicity, and good adaptability, biosurfactants play an important role in the fields of enhanced oil recovery, environmental bioremediation, food processing, and pharmaceuticals. play an important role. [0003] The production of biosurfactants by bioreactor fermentation is a complex biochemical reaction process. The type, quality and quantity of biosurfactants depend on the composition of the culture medium and fermentation process conditions, such as: pH, temperature, stirring, ventilation, dilution rate, Concentration of metal ions, types of carbon and nitrogen sources. Different elements such as iron and manganese affect the production of biosurfactants. Ad...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04C12R1/125C12R1/385
CPCC12P1/04C12R2001/385C12N1/205C12R2001/125
Inventor 牟伯中刘金峰杨世忠周光威
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com