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A method for detecting multiple mycotoxins in livestock and poultry feces samples

A technology for mycotoxins and feces samples, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of less simultaneous detection and difficulty in meeting the detection limit, and achieve the effect of simple pretreatment process, low cost and safe operation

Active Publication Date: 2021-05-11
SHANGHAI ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the research focus of scholars is mainly on aflatoxins, ochratoxins and fusarium toxins, and the existing mycotoxin detection methods mainly focus on food raw materials, seldom detect multiple toxin metabolites in animal feces at the same time, and the detection limit It is also difficult to meet the needs of food safety monitoring

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  • A method for detecting multiple mycotoxins in livestock and poultry feces samples
  • A method for detecting multiple mycotoxins in livestock and poultry feces samples
  • A method for detecting multiple mycotoxins in livestock and poultry feces samples

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Embodiment 1

[0061]1, experimental materials and methods

[0062](1) Instruments, reagents and materials

[0063]TQS triple four-stage rod chromatography collapse series spectrometer (US Waters); XYC-12A nitrogen blower (Shanghai Qianxing Analysis Instrument Co., Ltd.); Milli-Q ultra pure water instrument (Millipore, USA); LL 3000 frozen Dryer (Denmark Heto PowerDry); Heraeus Multifuge X 3 High-speed Centrifuge (American THERMO FISHERSCIENTIFIC); Ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); High Speed ​​Crusher (Shanghai Dingguang Machinery Equipment Co., Ltd.); Electronic Analysis Balance (Germany SARTORIUS); vortex oscillator, homogeneous machines are commonly used in laboratory.

[0064]Methanol, acetonitrile (chromatography, Germany Merck); Acetic acid, ammonium acetate (chromatographic, US ALADDIN); 100000Uβ-plasmate (US Sigma-Aldrich), 50 mmol / L a acetate solution is made into 10000uβ- Glucose canolazenase buffer preserved at 4 ° C; Fungi toxin standard (US Sigma-Aldrich): FB1, D...

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Abstract

The invention discloses a method for detecting multiple mycotoxins in livestock and poultry feces samples. After the samples are enzymolyzed by β-glucuronidase, they are treated with acetonitrile / water solution (84 / 16, v / v, containing 10% NaCl) ultrasonic extraction, centrifugation, concentration, reconstitution, and then gradient elution was carried out directly in the TQS triple quadrupole liquid chromatography tandem mass spectrometer with methanol and 5 mmol / L ammonium acetate aqueous solution (containing 0.1% formic acid) as the mobile phase Analysis, 15 kinds of mycotoxins can be effectively separated. The method of the present invention has no complicated derivation and purification steps, has the characteristics of simplicity, quickness, cost-efficiency and high efficiency, can simultaneously and rapidly detect multiple mycotoxins in samples, and can effectively detect the residual amount of mycotoxins in samples.

Description

Technical field[0001]The present invention relates to the field of food safety testing, and in particular, to a method for detecting a variety of fungal toxins in a sample of livestock and funeral.Background technique[0002]Fungal toxins, is a class of toxic secondary metabolites produced by molds in the growth process, which has great harm to people and animals. There are more than 400 fungal toxins currently reported in nature, which are widely used in all kinds of food grains and other foods, resulting in a large number of feed raw materials, feed or food being contaminated, which is often used as food, feed or feed raw materials. The corn, soy, bran, etc., especially prone to mildew, which is prone to fungal toxin. Animal food is widely loved by its unique taste and rich nutrients. When these contaminated raw materials are fed by bad merchants, feeding animals, they will pass genetics, proteins and in their body. The synthesis of various enzymes is inhibited and the role of damag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 杨俊花凌阿茹郭文博赵志辉孙真真
Owner SHANGHAI ACAD OF AGRI SCI
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