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Geobacillus ts-1 and its application

A technology of Geobacillus and TS-1, which is applied in the direction of bacteria, natural water treatment, mining wastewater treatment, etc., can solve the problems of limited application range in mine field, difficult desulfurization performance, sewage environmental adaptability, and different performance, etc., to achieve Guarantee the effect of development, reduce corrosion, and improve the effect of water quality

Active Publication Date: 2021-07-09
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the desulfurization strains screened have different performances, and it is difficult to have excellent desulfurization performance and good sewage environment adaptability at the same time. Therefore, the application range of the mine is greatly limited, especially for high-temperature sewage, the thermophilicity of the strains becomes very important

Method used

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  • Geobacillus ts-1 and its application
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  • Geobacillus ts-1 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Screening of Geobacillus sp. TS-1 (CGMCC No.15607) strain.

[0035] Get the sewage of Shengli Oilfield Marine Oil Production Plant, get 1ml with a disposable syringe, and inoculate it into a liquid medium (the composition of the medium: Na 2 S·9H 2 O 0.001wt%, sodium citrate 0.5%, KNO 3 0.2wt%, K 2 HPO 4 0.1wt%, KH 2 PO 4 0.1wt%, MgSO 4 : 0.02wt%, the balance is water, the pH value is 7.2, the balance is water, the pH value is 7.2, feed sterile nitrogen, and simultaneously heat to remove the oxygen in the medium, sterilize at 121°C for 30min), 70°C Culture 7d. Use a disposable syringe to take 1 mL of the culture solution after 7 days, and inoculate it into a new liquid medium (Na 2 S·9H 2 O 0.002%, the contents of other components remain unchanged), cultured at 70°C for 7 days. Get 1ml of the culture solution after 7 days, insert new culture medium again (Na 2 S·9H 2 O 0.003%, the contents of other components remain unchanged), and cultivated at 70°C for 7...

Embodiment 2

[0039] Morphological, physiological and biochemical characteristics of Geobacillus sp. TS-1 (CGMCC No.15607).

[0040] 1. Test strains

[0041] Geobacillus sp. TS-1 (CGMCC No. 15607) strain isolated in Example 1 of the present invention.

[0042] 2. Experimental method

[0043] Refer to the experimental method of "Bergey's Manual of Systematic Bacteriology" to detect its Gram staining, cell size and shape, growth temperature, growth pH range, and NaCl tolerance. Carry out methyl red, catalase, V-P, glucose acid production, starch hydrolysis, pyocyanin, oxidase experiments, nitrate reduction, nitrite reduction experiments, and the use of acetate, arabinose, galactose, xylose, Inosose, lactose, rhamnose, sucrose and mannose experiments.

[0044] 3. Experimental results

[0045] The results showed that the bacterium was Gram-positive, rod-shaped under the microscope, facultatively anaerobic, with a growth temperature of 55-75°C, the most suitable growth temperature was 70°C, ...

Embodiment 3

[0047] Experiment of the removal performance of Geobacillus sp. TS-1 (CGMCC No.15607) on sulfide.

[0048] 1. Test strains

[0049] Geobacillus sp. TS-1 (CGMCC No. 15607) strain isolated in Example 1 of the present invention.

[0050] 2. Experimental method

[0051] Pick single bacterium colony and insert in 100ml liquid culture medium (substrate composition: Na 2 S·9H 2 O0.001wt%, sodium citrate 0.5%, KNO 3 0.2wt%, K 2 HPO 4 0.1wt%, KH 2 PO 4 0.1wt%, MgSO 4 : 0.02wt%, the balance is water, the pH value is 7.2, feed sterile nitrogen, and simultaneously heat to remove the oxygen in the culture medium, sterilize at 121°C for 30min), cultivate overnight at 70°C until the logarithmic growth phase. Prepare oilfield sewage with a sulfide content of 30mg / L, inoculate the cultivated bacterial solution into a triangular flask containing 100ml of oilfield sewage at an inoculation amount of 2%, place it in a 70°C incubator, and test the sulfide in it every 12 hours. The conc...

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Abstract

The invention discloses a strain of Geobacillus sp. TS-1 and its application. The strain of the invention is obtained from the sewage sample of the Shengli Oilfield Marine Oil Production Plant, which is obtained through enrichment and screening under anaerobic conditions at 70°C, and Obtained through repeated screening and subculture through desulfurization performance investigation, it is named Geobacillus sp.TS‑1, and its preservation number is CGMCC No.15607. The invention further discloses their application in oil field sewage treatment. The test results show that the geobacillus or its bacterial agent of the present invention can effectively remove sulfide in sewage, reduce corrosion and improve sewage water quality. Used for the treatment of polymer sewage prepared in oilfield polymer flooding, the Geobacillus or its bacterial agent of the present invention can effectively solve the problem of viscosity reduction caused by sulfides, and ensure the development effect of polymer flooding.

Description

technical field [0001] The invention belongs to the fields of oilfield sewage treatment technology and environmental biotechnology, and in particular relates to a strain of Geobacillus TS-1 and its application in oilfield sewage treatment. Background technique [0002] The existence of sulfide in oilfield wastewater will cause various hazards. The existence of sulfide will cause serious corrosion of equipment, pipelines and other metal materials, resulting in equipment damage, and its corrosion product ferrous sulfide black precipitation will cause the suspension of sewage to rise and form dirt, leading to deterioration of water quality; at the same time, sulfide in sewage The existence of polymer flooding will lead to a significant decrease in the viscosity of the polymer flooding solution, which not only wastes a large amount of polymer dry powder, but also seriously restricts the development of polymer flooding field tests. [0003] In view of this situation, the current...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10C02F101/30C02F103/38C02F103/10
CPCC02F3/34C02F3/345C02F3/348C02F2101/101C02F2101/40C02F2103/10C02F2103/38C12N1/20C12N1/205C12R2001/01
Inventor 张绍东徐闯曹嫣镔徐鹏张守献潘永强袁长忠冯逸茹宋永亭林军章
Owner CHINA PETROLEUM & CHEM CORP
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