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Cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling

A kind of YUPP-10, cotton verticillium wilt technology, applied to cotton endogenous bacteria YUPP-10 and its application field in cotton verticillium wilt prevention and treatment, to achieve the effects of convenient use, short production cycle and low production cost

Inactive Publication Date: 2018-10-16
INST OF COTTON RES CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, biological control has achieved certain results in the prevention of cotton Verticillium wilt, but many difficulties have been encountered in actual use, such as how to maintain long-term disease resistance; how to normally colonize and exert disease-resistant effects with biocontrol agents; How to smoothly apply biocontrol agents to target sites

Method used

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  • Cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling
  • Cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling
  • Cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The isolation of embodiment 1 bacterial strain

[0022] (1) Isolation of strains

[0023] Collect phenotypically disease-free cotton plants in cotton fields, clean the surface of the cotton plants, peel off the skin, and cut them into 2-3cm long sections. The following steps are completed in an ultra-clean workbench. Disinfect the peeled cotton plants with 70% ethanol for 1 min, wash 3 times with sterile water, sterilize with 3% sodium hypochlorite for 4 min, wash 3 times with sterile water, and sterilize the cotton stalks Cut into pieces and grind, make a suspension with sterile water, spread it on an LB plate, incubate at 37°C for 24 hours, pick a single colony, pick a single colony with differences according to its shape, size, color, etc. Cultivate at 37°C for 24 hours on the collection medium plate, and select the bacteria that can grow on the enrichment medium for further pure culture. The purified strains were preserved.

[0024] (2) Characteristics of YUPP-10...

Embodiment 2

[0032] Example 2 The inhibitory effect of YUPP-10 on Verticillium dahliae

[0033] (1) Test method

[0034] 1. Inhibitory effect of whole metabolites of YUPP-10 on Verticillium dahliae

[0035] Introduce YUPP-10 into LB liquid medium, shake and culture in a constant temperature shaker at 37°C and 180rpm / min for 24h. Inoculate Verticillium dahliae in the center of different PDA plate culture medium, punch holes in the PDA plate symmetrically in a triangular shape, each point is 20 mm away from the center, add 100 μL of YUPP-10 culture medium to two points, and add 100 μL of LB liquid to the remaining point The culture medium was used as a control, cultured at a constant temperature of 25°C to observe the antibacterial situation, and the colony radius of Verticillium dahliae was measured after 7 days. The following formula was used to calculate the relative inhibition rate of YUPP-10 on the growth of pathogenic fungi, and each treatment was repeated three times.

[0036] Colo...

Embodiment 3

[0048] Example 3 The control effect of YUPP-10 on cotton verticillium wilt

[0049] (1) Test method

[0050] 1. Preparation of YUPP-10 bacterial fertilizer

[0051] The preserved YUPP-10 was inoculated on LB solid medium, and activated at 37°C for 18 hours; the activated strain was inoculated in LB liquid medium, shaken and cultured at 37°C, 180rpm / min for 18h to make strains liquid; inoculate the YUPP-10 strain liquid in sterilized vermiculite corn culture medium Kernel flasks, culture at 37°C for 72 hours, take out the culture, and air-dry it in a cool place to make YUPP-10 bacterial fertilizer for use.

[0052] 2. Cultivation of cotton seedlings

[0053] The cotton seeds were sterilized with 10% hydrogen peroxide for 10 minutes, washed three times with sterile water, and soaked in sterile water at room temperature for 24 hours. The disease-resistant variety Lumianyan No. 21 was used as the test variety. The cotton seedling cultivation method refers to the vermiculite san...

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Abstract

The invention relates to the field of plant pathology, in particular to cotton endophytic bacteria YUPP-10 and application thereof in cotton verticillium wilt controlling. The strain preservation number of the cotton endophytic bacteria YUPP-10 is CCTCC No:M2017141. The experimental result shows that the cotton endophytic bacteria YUPP-10 has inhibitory effect on the growth of verticillium wilt pathogen, corn vermiculite culture of the cotton endophytic bacteria YUPP-10 has a high controlling effect on the verticillium wilt, and the cotton endophytic bacteria YUPP-10 is suitable as a biological resource for verticillium wilt controlling. The cotton endophytic bacteria YUPP-10 and application in cotton verticillium wilt controlling provide new resources for enriching biological controllingof agricultural diseases and has practical application guidance for pollution-free green agricultural systems.

Description

technical field [0001] The invention relates to the field of plant pathology, in particular to cotton endophytic bacteria YUPP-10 and its application in the control of cotton verticillium wilt. Background technique [0002] Verticillium wilt (Verticillium wilt), as a vascular fungal disease, is the main disease causing cotton yield reduction. However, due to the characteristics of the pathogen's own transmission and the complex pathogenic mechanism, it is still difficult to control it. [0003] Cotton verticillium wilt caused by Verticillium dahliae is a difficult problem worldwide. The occurrence of cotton verticillium wilt is related to the abnormal soil microflora and the increase of pathogenic bacteria in the root zone caused by continuous cotton cropping. Due to the lack of natural verticillium wilt-resistant provenance and resistance-transgenic varieties are easy to degenerate, disease-resistant breeding lacks breakthrough high-resistant varieties. The use of chemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/085
CPCA01N63/00C12N1/205C12R2001/085
Inventor 朱荷琴周京龙周燚冯自力冯鸿杰孙正祥魏锋李志芳师勇强赵丽红
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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