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Method for preparing Chinese chive seed oil and polypeptide

A leek seed, a clean technology, applied in the field of natural product chemistry and biology, can solve the problems of waste of resources, environmental pollution, etc., and achieve the effect of good variety of biological activities and good stability

Inactive Publication Date: 2018-10-16
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The residue after processing is still rich in a large amount of protein, and direct disposal not only wastes resources, but also causes environmental pollution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A preparation method of leek seed oil and polypeptide, comprising the following steps:

[0016] 1. Take 20kg of clean leek seeds, fry them with slow fire until there is a crackling sound, spray salt water while frying, use 0.4kg of salt, fry them dry, and store them in a ventilated and dry place for later use;

[0017] 2. Place the processed leek seeds in a 40L extraction kettle for extraction, the temperature is 40°C, the pressure is 25MPa, the temperature of the separation kettle is 35°C, the pressure of the separation kettle is 10.0MPa, the extraction time is 2h, CO 2 The flow rate is 2.5L / h, and the oil yield is 21%;

[0018] 3. Then dry the chive seeds after oil extraction, and crush them into 30-mesh chive seed meal; add Aspergillus niger spore suspension and fermentation medium, adjust the pH to 3.5, the material-to-liquid ratio Kg / L is 1:10, and the fermentation time is 48 hours. Then the filtrate was centrifuged to obtain the leek seed polypeptide extract; the...

Embodiment 2

[0020] A preparation method of leek seed oil and polypeptide, comprising the following steps:

[0021] 1. Take 20kg of clean leek seeds, fry them with slow fire until there is a crackling sound, spray salt water while frying, use 0.4kg of salt, fry them dry, and store them in a ventilated and dry place for later use;

[0022] 2. Put the processed leek seeds in a 40L extraction kettle for extraction, the temperature is 40°C, the extraction pressure is 10MPa, the temperature of the separation kettle is 35°C, the pressure of the separation kettle is 10.0MPa, the extraction time is 2h, CO 2 The flow rate is 2.5L / h, and the oil yield is 16%;

[0023] 3. Then dry the leek seeds after oil extraction, and crush them into 30-mesh leek seed meal; add Aspergillus niger spore suspension and fermentation medium, adjust the pH to 4.5, the ratio of material to liquid Kg / L is 1:10, and the fermentation time is 48h , and then centrifuged to take the filtrate, which is the leek seed polypeptid...

Embodiment 3

[0025] A preparation method of leek seed oil and polypeptide, comprising the following steps:

[0026] 1. Take 20kg of clean leek seeds, fry them with slow fire until there is a crackling sound, spray salt water while frying, use 0.4kg of salt, fry them dry, and store them in a ventilated and dry place for later use;

[0027] 2. Put the processed leek seeds in a 40L extraction kettle for extraction, the temperature is 40°C, the extraction pressure is 30MPa, the temperature of the separation kettle is 35°C, the pressure of the separation kettle is 10.0MPa, the extraction time is 2h, CO 2 The flow rate is 2.5L / h, and the oil yield is 22%;

[0028] 3. Then dry the chive seeds after oil extraction, crush them into 30-mesh chive seed meal; add Aspergillus niger spore suspension and fermentation medium, adjust the pH to 2.5, the ratio of material to liquid Kg / L is 1:10 and the fermentation time is 48h, Then the filtrate was centrifuged to obtain the leek seed polypeptide extract; t...

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Abstract

The invention discloses a method for preparing Chinese chive seed oil and polypeptide. The method comprises the following steps: frying clean Chinese chive seeds with soft fire till oil crackling sound, and adding salt water to brew the Chinese chive seeds while frying; extracting the brewed Chinese chive seeds by using a supercritical CO2 extraction technique so as to extract Chinese chive seed oil from the Chinese chive seeds; further extracting the Chinese chive seeds after the Chinese chive seed oil is extracted, crushing the Chinese chive seeds into Chinese chive seed bran, adding water and aspergillus niger, carrying out liquid fermentation, and finally carrying out centrifugation, thereby obtaining filtrate, namely a Chinese chive seed polypeptide extract. The Chinese chive seed polypeptide prepared by using the method has good stability and diverse biological activities, and has great significances in development and application of novel massive health products.

Description

technical field [0001] The present invention belongs to the field of natural product chemistry and biology. Specifically, it is a preparation method of leek seed oil and polypeptide. Background technique [0002] Leek is a plant of the Allium family, which is grown all over the country. Leek seeds are its dry, ripe seeds. Leek seeds are dark in color, flat and round in appearance, wrinkled on the surface, hard in quality, and have the characteristic smell of leeks. It has the effects of anti-inflammatory and cough, expectorant and asthma, nourishing liver and kidney, warming waist and knees, etc. It can be used to treat lower abdomen pain, diabetes and other symptoms; so it has high nutritional and medicinal value. [0003] Leek seeds not only contain a variety of unsaturated fatty acid oils such as linoleic acid, oleic acid, etc., but also contain active ingredients such as sulfides, alkaloids, and vitamins. Chive seed oil is a health vegetable oil with a special aroma ...

Claims

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Application Information

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IPC IPC(8): C11B1/04C11B1/10C12P21/06C12R1/685
CPCC11B1/04C11B1/104C12P21/06Y02P20/54
Inventor 王立升周中杰梁鹏云刘旭罗轩江俊杨华陈海燕林翠梧吴黎川
Owner GUANGXI UNIV
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