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Application of total alkaloids of dendrobium stem in preparation of anti-aging drugs

A technology of Dendrobium nobile and biological total alkaloids, which is applied in the field of biomedicine and can solve the problems of no anti-aging drugs and poor compliance

Inactive Publication Date: 2018-10-12
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-drug treatment emphasizes long-term participation in fitness exercises, eating more foods containing nucleic acids, and starvation, but the compliance is poor
The research and development of anti-aging drugs has always attracted much attention, but so far there is still no anti-aging drug approved by the drug administration department

Method used

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  • Application of total alkaloids of dendrobium stem in preparation of anti-aging drugs
  • Application of total alkaloids of dendrobium stem in preparation of anti-aging drugs
  • Application of total alkaloids of dendrobium stem in preparation of anti-aging drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment one: the extraction method of Dendrobium nobile total alkaloids

[0034] 1. The extraction method of Dendrobium nobile total alkaloids

[0035] 1.1 Extraction of fat-soluble total alkaloids

[0036] Precisely weigh 5g of dendrobium sample powder and place them in 250ml Erlenmeyer flasks with stoppers, add appropriate amount of concentrated ammonia water, moisten, place in airtight place for 30min, add 100ml of chloroform, reflux extraction for 2h, suction filter, recover chloroform, and obtain.

[0037] 1.2 Extraction of water-soluble total alkaloids

[0038] Accurately weigh 5g of each sample powder of Dendrobium dendrobii and place them in 250ml conical flasks with stoppers, add appropriate amount of concentrated ammonia water, moisten, place for 30min, add n-butanol 100ml, reflux extraction for 2h, suction filter, recover n-butanol, and obtain .

[0039] 1.3 Identification of Dendrobium total alkaloids

[0040] Use a micro-spotter to draw 20 μl of each...

Embodiment 2

[0065] Embodiment two: the anti-aging pharmacodynamics experiment of total alkaloids of Dendrobium nobile

[0066] 1. Materials and methods

[0067] 1. Experimental materials

[0068] 1.1 Main instruments: -80°C low-temperature freezer (Japan Sanyo Company), Milli QA pure water processor (Millipore Company), LRH-250 biochemical incubator (Shanghai Qixin Scientific Instrument Co., Ltd.), MJ series mold incubator (Beijing North Lihui Experimental Instrument Equipment Co., Ltd.), TS series constant temperature shaker (Beijing North Lihui Experimental Instrument Equipment Co., Ltd.), etc.

[0069] 1.2 Reagents and medicines:

[0070] Total alkaloids of Dendrobium nobile: The raw materials used for research are 8-year-old Dendrobium nobile produced in Chishui, Zunyi. After the raw materials were crushed, the total alkaloids were separated and extracted by solvent method. The purity was 78.9% as determined by UPLC-MS. Tryptone (Beijing Suolaibao Technology Co., Ltd.), agar powder...

Embodiment 3

[0091] Example 3: Effect of total alkaloids of Dendrobium nobile on galactose-induced aging mouse model

[0092] In this experiment, by observing the preventive effect of total alkaloids of Dendrobium nobile on total alkaloids of Dendrobium nobile, and exploring its mechanism of action, it provides basic pharmacological basis for the development and research of total alkaloids of Dendrobium nobile for anti-aging.

[0093] 1. Materials and methods

[0094] 1. Experimental materials

[0095] 1.1 Experimental equipment: 721 type ultraviolet-visible spectrophotometer: Shanghai Shenhua Instrument Automation Co., Ltd.; BI2000 Morris water maze: Chengdu Taimeng Technology Co., Ltd.; DT-6 mouse jumping platform tester: Chengdu Taimeng Technology Co., Ltd.; BA-6 Mouse dark avoidance tester: Chengdu Taimeng Technology Co., Ltd.; -80°C low-temperature freezer: Forma Scientic, Germany; Milli QA pure water processor: Millipore; 3K30 high-speed refrigerated centrifuge: Sigma, Germany; TOM...

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Abstract

The invention discloses an application of total alkaloids of dendrobium stem in preparation of anti-aging drugs and belongs to the technical field of biomedicine. According to the application, total alkaloids of dendrobium stem are extracted and the content is measured. Through the caenorhabditis elegans anti-aging pharmacodynamics experiment and galactose-caused influence on an aging mouse modeland SAMP8 mouse brain aging protection and mechanism study, it is proved that the total alkaloids of dendrobium stem can improve the learning and memory declining of SAMP8 mice, enhance exercise tolerance, attenuate brain atrophy in SAMP8 mice and reduce the neuronal loss. The results of the experiment are similar to those of the anti-aging drug metformin. The total alkaloids of dendrobium stem have anti-aging effects and the alkaloid action mechanism is related to up-regulation of anti-aging protein Klotho expression, reduction of A beta aggregation and induction of autophagy activity. The experiment results fully prove the anti-aging effects of the total alkaloids of dendrobium stem in anti-aging drugs.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to the extraction of total alkaloids from Dendrobium nobile and its application in the preparation of anti-aging medicines. Background technique [0002] Senescence, also known as aging, is a spontaneous and inevitable process of all multicellular organisms over time, with harmful changes at all levels of the body and tissues, and a process of decline in function, adaptability and resistance , can be divided into two types: physiological aging and pathological aging. Physiological aging refers to the irreversible complex morphological structure and physiological functions of the whole body that occur with age and are affected by genetic factors. Degenerative changes, also known as normal aging. Pathological aging refers to accelerated aging caused by diseases or abnormal factors, also known as abnormal aging. There is an inseparable relationship between aging and senile diseas...

Claims

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Application Information

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IPC IPC(8): A61K36/8984A61P39/06
CPCA61K36/8984A61P39/06
Inventor 石京山吴芹刘波李利生刘静吕玲丽
Owner ZUNYI MEDICAL UNIVERSITY
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