Fast extraction method of immunoglobulin Y, anti-influenza product prepared by immunoglobulin Y, and application of immunoglobulin Y

Abstract

The invention discloses a fast extraction method of immunoglobulin Y, an anti-influenza product prepared by the immunoglobulin Y, and application of the immunoglobulin Y. The fast extraction method includes: using a tetravalent influenza vaccine comprising H1N1, H3N2, B(Victoria) and B(Yamagata) and an escherichia coli staphylococcus aureus whole-cell vaccine to immunize laying hens; mechanicallyand automatically separating eggs to obtain yolk, and using a citric acid-disodium hydrogen phosphate buffer solution dilute and stabilize yolk liquid pH; adding dry ice to fast lower system temperature; performing continuous high-speed low-temperature centrifuging to remove fat particles so as to obtain immunoglobulin Y supernate; allowing the immunoglobulin Y supernate to sequentially pass double filter membrane layers with pore diameters being 2-6 micrometers and 0.3-0.8 micrometers respectively, using a hollow fiber ultrafiltration membrane with the molecular weight cut-off being 300kD toremove impure protein, and processing with a hollow fiber ultrafiltration membrane with the molecular weight cut-off being 100kD to obtain concentrated immunoglobulin Y liquid; adding auxiliary materials to prepare into an oral and nasal mucosa spray. The method is high in automation level, low in energy consumption, high in yield, green and environmentally friendly, capable of achieving large-scale industrial production and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, especially the biotechnology of obtaining antibodies through immunization and breeding technology and preparing them into medicines or health care products, and particularly relates to a method for rapidly extracting egg yolk antibodies, an anti-influenza product prepared from the extracted egg yolk antibodies and its application. Background technique [0002] Influenza is an acute respiratory infectious disease caused by a virus. It is characterized by sudden fever, cough, headache, muscle and joint pain, severe discomfort, sore throat and runny nose. It is mainly spread through contact and air droplets. Currently, influenza A virus subtypes H1N1 and H3N2 and influenza B virus strains B / Yamagata and B / Victoria are the main strains that cause seasonal influenza in humans. At present, vaccination is used for prevention, and neuraminidase inhibitors and M2 protein inhibitors are used for treat...

AI Technical Summary

Problems solved by technology

For example: collecting egg yolk by hand-beating eggs is inefficient and not suitable for large-scale use, and it is easy to introduce pollution sources, increasing the risk of contamination of egg yolk antibodies; using repeated freezing and thawing 1-3 times, if used The production operation is time-consuming and laborious; use organic solvents such as caprylic acid or chloroform to extract IgY, the application of organic solvents affects the activity of IgY, and is toxic, pollutes the environment, and is inconvenient to operate; adopts ammonium sulfate salting out precipitation method, there are centrifugation to collect precipitation and Time-consuming operations such as reconstitution and precipitation; the water dilution extraction method is used to extract IgY, although it is suitable for large-scale production and protection of IgY activity, but the extraction time is prolonged. Lowered to 0-8°C, the production process is time-consuming and energy-consuming in actual production, and the efficiency of large-scale industrial production is low

Images