Nanometer composite material and applications of nanometer composite material in tracing of lymph nodes
A nano-composite material and nano-material technology, which can be used in medical preparations containing active ingredients, wave energy or particle radiation treatment materials, drug combinations, etc.
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Embodiment 1
[0081] Embodiment 1: the preparation of core nanomaterial
[0082] core material bTiO 2 Preparation of -1
[0083] According to the method in the literature (Energy Environ.Sci., 2013, 6, 3007–3014), the aluminum reduction black TiO was prepared 2 , denoted as the kernel bTiO 2 -1.
[0084] core material bTiO 2 Preparation of -2
[0085] Take 0.1mol / L titanium tetrachloride (TiCl 4 ) hydrochloric acid solution 50mL, slowly added dropwise to 90mL ultrapure water, stirred and hydrolyzed for 8 hours under ice bath conditions. After the hydrolysis, the reaction solution was placed in a dialysis bag, dialyzed in pure water, and the water was changed every 3 hours, and the water was changed 4 times in total to obtain TiO 2 precursor solution. TiO 2 100mL of the precursor solution was placed in a reaction kettle and heated at 180°C for 10 hours to obtain TiO 2 Nanoparticles. TiO 2 After drying the nanoparticles at a high temperature of 60°C, TiO 2 Nanoparticle powder...
Embodiment 2
[0098] Example 2: Sample bTiO 2 -1- Preparation of hyaluronic acid nanocomposites
[0099] Take the kernel bTiO 2 - 50 mg of nanomaterials, dispersed in 200 mL of pure water, ultrasonically dispersed for 30 minutes. Then 200 ml of 1 mg / mL hyaluronic acid solution was added, and the reaction was stirred for 24 hours. After the reaction, centrifuge at 12,000 rpm for 10 minutes to remove excess hyaluronic acid and obtain the sample bTiO 2 -1- Hyaluronic acid composite nanoparticles.
Embodiment 3
[0100] Example 3: Sample bTiO 2 -1-mSiO 2 - Preparation of ICG-PEI nanocomposite
[0101] Take the kernel bTiO 2 -1 50 mg of nanomaterials was dissolved in 100 mL of pure water, and after ultrasonic dispersion for 30 minutes, solution A was obtained. Add 100 mg cetyltrimethylammonium bromide (abbreviated as CTAB) to solution A, stir and heat for 30 minutes to dissolve CTAB. After CTAB was dissolved, 3 mL of ethanol and 150 μL of NaOH (2M concentration) were added to the reaction system, and heated to 70°C. Add 200 μL tetraethyl orthosilicate (TEOS) to the reaction solution, stir the reaction for 1 hour, then add NH 4 NO 3 30 mg, after being incubated at 60°C for 1 day, centrifuged and washed at 12,000 rpm to obtain bTiO 2 -1-Mesoporous silicon composite. Take bTiO 2 -1-Mesoporous silica (abbreviated as mSiO 2 ) 50 mg of the composite material was dispersed into 100 mL of pure water, ultrasonically dispersed for 10 min, and then 20 ml of 0.1 mg / mL indocyanine green (a...
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