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Inactivated vaccine prepared by Newcastle disease virus heat stable strain and preparation method thereof

A technology of Newcastle disease virus and inactivated vaccines, which is applied in the field of biological product manufacturing, can solve the problems such as long time for formaldehyde inactivation, and achieve the effect of overcoming relatively poor thermal stability, broad application prospects, and good immunogenicity

Active Publication Date: 2018-08-10
INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Formaldehyde is generally used as an inactivating agent in China to prepare virus inactivated vaccines, but there are certain problems in inactivating formaldehyde. It is a strong irritating carcinogen, and it is necessary to ensure that the residual formaldehyde is completely removed.
Secondly, the inactivation time of formaldehyde is longer, and the inactivation effect is affected by many factors such as temperature and pH, so it is necessary to find a new inactivator

Method used

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  • Inactivated vaccine prepared by Newcastle disease virus heat stable strain and preparation method thereof
  • Inactivated vaccine prepared by Newcastle disease virus heat stable strain and preparation method thereof
  • Inactivated vaccine prepared by Newcastle disease virus heat stable strain and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment one: the preparation of the inactivated vaccine of Newcastle disease virus thermostable strain

[0030] 1. Preparation of viral allantoic fluid

[0031] Dilute the allantoic fluid of a stable strain of Newcastle disease virus 10 times, shake well, inoculate 9-11-day-old SPF chicken embryos through the allantoic cavity, inoculate 0.1mL per embryo, and continue to incubate for 5 days in an incubator at 37°C. Harvest The allantoic fluid was stored in a sterile container and stored at -20°C after sampling.

[0032] 2. Dilution of viral allantoic fluid

[0033] Prior to virus inactivation, virus quantification is required on the collected allantoic fluid diluted to 10 with the optimized dilution buffer. 8.0 EID 50 / ml. In this embodiment, the optimized dilution buffer refers to 100mmol / L Tris-HCl (pH 7.8), 5mmol / L sodium chloride, 5mmol / L potassium chloride, 2.0mmol / L calcium chloride, 1.0mmol / L Diluent consisting of L magnesium chloride and 1mmol / L disodium ...

Embodiment 2

[0063] Embodiment two: the shelf life test of vaccine

[0064] The prepared inactivated vaccine was placed in a 42°C incubator, and samples were taken every 10 days to detect the titer of HA. At the same time, the LaSota standard strain that was inactivated by the same method was set as the control group, and three replicates were set for each sample. Such as figure 2 As shown, the results show that the prepared inactivated vaccine still maintains good thermal stability at 42°C, and the hemagglutination titer of the virus does not change significantly after being placed for 60 days, while the hemagglutination titer of the control group LaSota has changed from the initial 2 8 down to 20. It shows that the prepared inactivated vaccine has good thermal stability.

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Abstract

The invention discloses an inactivated vaccine prepared by a Newcastle disease virus heat stable strain and a preparation method thereof. The vaccine takes the Newcastle disease virus heat stable strain as a vaccine virus seed. The preparation method comprises the following steps: preparing viral allantoic fluid of the Newcastle disease virus heat stable strain, diluting the viral allantoic fluidwith optimized dilution buffer, and preparing and inspecting beta-propiolactone and oil-adjuvant inactivated vaccine. The heat stability of the vaccine is obviously higher than that of the other conventional inactivated vaccines, and the inactivated vaccine also has excellent immunogenicity, solves the problems of relatively poor stability, high dependency level on a cold chain system and the likein the conventional common Newcastle disease virus vaccine, and has wide application prospects.

Description

technical field [0001] The invention relates to a vaccine preparation method, in particular to a novel inactivated vaccine prepared from a heat-stable strain of Newcastle disease virus and a preparation method thereof. It belongs to the field of biological product manufacturing. Background technique [0002] Newcastle disease is a highly contact and acute septicemia of poultry caused by Newcastle disease virus, which has a high incidence and mortality rate. It is regulated by the International Office of Epizootics as one of the only two A-type poultry diseases. Epidemics have occurred worldwide, causing huge economic losses to the world. Newcastle disease virus (NDV) was isolated for the first time in my country in 1946, and it is now common throughout the country, and it is one of the poultry diseases that cause the most harm and economic loss to the poultry industry in my country. At present, vaccination is the main method used in my country To control the occurrence of N...

Claims

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Application Information

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IPC IPC(8): A61K39/17A61P31/14C12N7/04
CPCA61K39/12A61K2039/5252A61P31/14C12N7/00C12N2760/18134C12N2760/18163
Inventor 温国元邵华斌李丽李林涛罗青平王红琳张腾飞汪宏才张蓉蓉罗玲卢琴商雨张文婷
Owner INST OF ANIMAL SCI & VETERINARY HUBEI ACADEMY OF AGRI SCI
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