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Lactobacillus salivarius specificity culture medium and application thereof

A technology of Lactobacillus salivarius and liquid culture medium, applied in the field of Lactobacillus salivarius specific culture medium, can solve the problems of no specific culture medium, hinder the breeding and development of probiotic bacteria, etc. The effect of improving screening efficiency

Inactive Publication Date: 2018-07-31
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no similar specific medium for Lactobacillus salivarius, which greatly hinders the selection and development of this type of probiotics

Method used

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  • Lactobacillus salivarius specificity culture medium and application thereof
  • Lactobacillus salivarius specificity culture medium and application thereof
  • Lactobacillus salivarius specificity culture medium and application thereof

Examples

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Embodiment example 1

[0031] Implementation case 1 The screening yield of Lactobacillus salivarius by conventional lactic acid bacteria culture medium

[0032] At present, Lactobacillus salivarius does not have a specific specific culture medium, and the main use is the universal medium of Lactobacillus for screening and isolation. Therefore, this case studies the screening yield of the universal medium for Lactobacillus salivarius .

[0033] The formula of the Lactobacillus universal medium (ie Glu-VAN-MRS medium) involved in this example: peptone 10g / L, yeast extract 5g / L, glucose 20g / L, anhydrous sodium acetate 2g / L , Diamine hydrogen citrate 2g / L, K 2 HPO 4 ·3H 2 O2.6g / L, MgSO 4 ·7H 2 O 0.5g / L, MnSO 4 ·7H 2 O 0.25g / L, Tween-80 1g / L, distilled water 1000g / L, pH 5 .0, vancomycin 0.02g / L.

[0034] MRS growth medium formula: peptone 10g / L, yeast extract 5g / L, glucose 20g / L, anhydrous sodium acetate 2g / L, hydrogen citrate diamine 2g / L, K 2 HPO 4 ·3H 2 O 2.6g / L, MgSO 4 ·7H 2 O 0.5g / L, MnSO 4 ·7H 2 O 0.25g / ...

Embodiment example 2

[0053] Implementation case 2 Analysis of the utilization capacity of different lactobacilli on carbon sources

[0054] According to the Berger’s Handbook’s characterization of the characteristics of various lactobacilli, it is found that different lactobacilli have different abilities to utilize various carbon sources. According to relevant records, there are four types of rhamnose, raffinose, mannitol, and sorbitol. The carbon source has a high degree of discrimination for Lactobacillus salivarius, but the manual does not indicate the sugar utilization of all the above-mentioned lactobacilli. Therefore, this case examines the utilization of these four types of sugar by the above-mentioned 9 types of lactobacilli, and selects them separately. Take one or several strains of 9 kinds of Lactobacillus for experiment.

[0055] Modified carbon source medium formula: peptone 10g / L, yeast extract 5g / L, carbon source 20g / L, anhydrous sodium acetate 2g / L, hydrogen citrate diamine 2g / L, K 2 H...

Embodiment example 3

[0067] Implementation case 3 Analysis of the enrichment ability of Lactobacillus in different carbon sources

[0068] According to Example 2, there are 6 types of intestinal lactobacilli that cannot use rhamnose and sorbitol, but these two sugars cannot distinguish between Lactobacillus salivarius, Lactobacillus rhamnosus, Lactobacillus plantarum and Lactobacillus paracasei, considering Whether the enrichment ability of these four kinds of Lactobacillus in the medium containing these two different carbon sources is different, as to whether the enrichment ability of Lactobacillus salivarius is better than the other three kinds of bacteria, it is controlled by the difference in abundance after enrichment Can the dilution achieve the effect of a sieve? Therefore, in this case, the glucose-containing medium was used as the positive control to investigate the enrichment of different bacteria in the liquid medium containing sorbitol or rhamnose under the condition of the same inoculati...

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Abstract

The invention discloses a lactobacillus salivarius specificity culture medium and application thereof. The culture medium is characterized in that an MRS culture medium is treated as the basis and isimproved, namely, beef extract is removed, the carbon resource is replaced by rhamnose, and antibiotics including vancomycin and streptomycin are added. The culture medium is simple and convenient toprepare, and low in cost; lactobacillus salivarius can be subjected to separating culture, and the viable stains can be counted; the method is reliable in counting result, simple to operate, and low in requirements on experiment people; and meanwhile, the abundance of the lactobacillus salivarius in a sample can be effectively improved during the separating and screening of human gut lactobacillus, and thus the yield of the lactobacillus salivarius is extremely increased. The method is suitably used for preparing and culturing the culture medium for separating lactobacillus salivarius and counting the viable stains.

Description

Technical field [0001] The invention relates to a specific culture medium for Lactobacillus salivarius and its application, and belongs to the field of microbial detection. Background technique [0002] Lactobacillus salivarius (Lactobacillus salivarius) belongs to the genus Firmicutes. The colony is smooth and round, milky white, with neat edges. It was originally isolated from the oral cavity, because it is widely present in the human intestine, oral cavity, vagina and other parts of the secretion In the liquid, it is considered to be the original bacteria of the human body. The Ministry of Health of the People's Republic of China listed Lactobacillus salivarius as a strain allowed for food in 2010, and the U.S. Food and Drug Administration (FDA) also listed it as one of the safe strains. [0003] A large number of studies have shown that Lactobacillus salivarius has good cell adhesion ability, acid and bile salt resistance, and has various physiological functions such as regula...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02C12Q1/06C12R1/225
CPCC12N1/02C12N1/20C12Q1/06
Inventor 翟齐啸陈卫陆文伟沈旭丹田丰伟赵建新张灏
Owner JIANGNAN UNIV
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