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Novel composite biological tissue repairing material as well as preparation method and application thereof

A technology for repairing materials and biological tissues, applied in medical science, prosthesis, etc., can solve the problems of insufficient mechanical properties of single-layer amniotic membrane, fast degradation time, peripheral nerve defects, etc., achieve broad clinical application and market prospects, and improve tensile strength. and tensile strength, reducing the effect of inflammatory response

Pending Publication Date: 2018-06-29
CHENGDU QINGSHAN LIKANG PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, single-layer amniotic membrane has defects such as insufficient mechanical properties and short degradation cycle, which limit its clinical application range.
According to clinical reports, because the amniotic membrane is thin, the tensile strength is insufficient, the tensile strength is weak, and the degradation time is fast, etc., it cannot be used for the repair of tissue defects that need to bear a certain mechanical load, such as various types of traumatic defects, congenital Defect, iatrogenic defect, etc., partial organ defect, peripheral nerve defect, ligament defect, etc.

Method used

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  • Novel composite biological tissue repairing material as well as preparation method and application thereof
  • Novel composite biological tissue repairing material as well as preparation method and application thereof
  • Novel composite biological tissue repairing material as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation of the composite biological tissue repair material of the present invention

[0041] 1.1 Preparation of experimental group A:

[0042] (1) Take the placental amniotic membrane of healthy puerperae, undergo defatting, virus inactivation, descaling, decellularization, and freeze-drying (prepared according to Example 1 of Publication No. CN1618954A), and then cut the acellular amniotic membrane after freeze-drying It is a rectangular unit of size 2cm x 4cm.

[0043] (2) Take a stainless steel flat plate and lay it flat on the operating table, first lay the first rectangular unit on the flat plate, and evenly apply 5% collagen solution on the upper surface of the material (0.2-0.3ml per square centimeter), The second rectangular unit is then overlapped with the first rectangular unit, and the air bubbles between the layers of each unit are removed to make them fit tightly.

[0044] (3) Repeat the above step (2) to overlap to 5 layers.

[0045] (4) a...

Embodiment 2

[0065] Example 2 Preparation of the composite biological tissue repair material of the present invention

[0066] Preparation of experimental group B:

[0067] (1) Take the placental amniotic membrane of healthy puerperae, undergo defatting, virus inactivation, descaling, decellularization, freeze-drying treatment (can be prepared according to Example 1 of publication number CN1618954A), and then cut the acellular amniotic membrane after freeze-drying It is a rectangular unit with a size of 4cm×8cm.

[0068] (2) Before wrapping, evenly spray α-cyanoacrylate medical adhesive on one side of each unit (smear 0.1-0.15ml per square centimeter), denoted as the Y side, and the one side that is not sprayed with adhesive The side is marked as N side.

[0069] (3) Take a cylindrical mold with a diameter of 1 mm, place the N side of the first rectangular unit towards the surface of the mold, and wrap the mold in a ring direction along the short side to remove the air bubbles between th...

Embodiment 3

[0072] Example 3 Preparation of the composite biological tissue repair material of the present invention

[0073] Preparation of experimental group C:

[0074] (1) Take the placental amniotic membrane of healthy puerperium, defatting, virus inactivating, decontaminating, and decellularizing to obtain an intermediate pint of cellular amniotic membrane (which can be prepared according to Example 1 of Publication No. CN1618954A).

[0075] (2) Take a concave mold, spread the first layer of intermediate product on the mold, and evenly apply 2% sodium hyaluronate solution (0.3-0.4ml per square centimeter) on the upper surface of the intermediate product; apply the second layer The intermediate product is laid flat on the first layer of material and stacked, and the air bubbles between the layers are eliminated to make it closely fit.

[0076] (4) Repeat step (2) to overlap and wrap to 4 units.

[0077] (5) another convex mold is taken and covered on the above-mentioned material, p...

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PUM

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Abstract

The invention discloses a novel composite biological tissue repairing material which is made of multiple layers of amnia, wherein the amnia are bonded together through an adhesive; and the adhesive isa medicinal adhesive of carboxymethylcellulose, a collagen solution, sodium hyaluronate, chondroitin sulfate and alpha-cyanoacrylate. The invention further discloses a preparation method and application of the composite material. The composite material disclosed by the invention is excellent in mechanical property, high in stability and high in biocompatibility, is not rapidly degraded, can be applied to soft tissue defection repairing and tissue postoperative adhesion prevention, has the advantages of reducing risks of reducing wound infection and inflammation reactions, and has excellent clinical application prospects.

Description

technical field [0001] The invention relates to a novel composite biological tissue repair material, a preparation method and application thereof, and belongs to the field of biological materials. Background technique [0002] Amniotic membrane is the innermost layer of fetal membrane, which belongs to a kind of biological membrane, and is a kind of natural extracellular matrix biological membrane material. The amniotic membrane is differentiated from the trophoblast layer. The surface is smooth, translucent, tough and elastic. The thickness is 0.02-0.50mm. Growth factors such as fibroblast growth factor-2 (FGF-2), transforming growth factor-β (TGF-β), vascular endothelial growth factor (VEGF) that promotes angiogenesis , also contains fibronectin (fibronectin, FN) and so on. The amniotic membrane does not express HLA-A, B, C and DR antigens or β2 microglobulin, and does not contain blood vessels, lymphatic and neural tissues, and its antigenicity is much lower than that o...

Claims

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Application Information

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IPC IPC(8): A61L27/36A61L27/24A61L27/20A61L27/50
CPCA61L27/20A61L27/24A61L27/3683A61L27/3687A61L27/3691A61L27/50A61L2300/412C08L1/286C08L5/08
Inventor 徐世兰侯鹏陈琳范琴刘凯唐莉
Owner CHENGDU QINGSHAN LIKANG PHARMA CO LTD
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