Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of dextran polymer, polymer micelle and drug carrier system

A technology of polymer glue and carrier system, which is applied in the direction of drug combination, cardiovascular system diseases, antipyretics, etc., can solve the problems of insufficient stability, limit the application of polymeric micelles, and poor targeting, and achieve good hydrogen peroxide Responsiveness, good responsiveness, damage reduction effect

Inactive Publication Date: 2020-02-14
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the existing polymeric micelles have defects such as poor targeting and insufficient stability when preparing tumor drug carriers, which limits the application of polymeric micelles in tumor drugs.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of dextran polymer, polymer micelle and drug carrier system
  • A kind of dextran polymer, polymer micelle and drug carrier system
  • A kind of dextran polymer, polymer micelle and drug carrier system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A kind of preparation method of dextran polymer and micelle with hydrogen peroxide responsiveness, its synthetic route is as follows figure 1 As shown, the specific steps are as follows:

[0060] 1) First react boroester with dodecanoic acid, under the protection of nitrogen, put boroester raw material A (1eq) and dodecanoic acid (1.5eq) into a 25mL round bottom bottle, and dissolve with DCM. Weigh DMAP (0.2eq) EDCI (1.5eq) and Et3N (0.5eq) in order and dissolve them in DCM respectively, then inject into the system with a syringe. The reaction solution was reacted at room temperature for 18 hours, followed by TLC spot plate. After the reaction is complete, add water to quench. The aqueous layer was extracted 4 times with DCM, the organic phases were combined, and the 4 Dry, filter with suction, and spin-dry to obtain the crude product, which is then purified by column chromatography. After passing through the mobile phase of PE:EA=50:1 to remove impurities, use PE:EA...

Embodiment 2

[0066] Use fluorescent probe technology to measure the critical micelle concentration (CMC) of micelle described in embodiment 1, concrete steps are as follows:

[0067] 1) Preparation of methanol solution of fluorescent probe pyrene

[0068] Dissolve 0.0051 g of pyrene in 25 ml of methanol to obtain a pyrene solution with a concentration of 1.01×10-3 mol / L, and then dilute it with methanol to obtain a pyrene solution of 1.616×10-6 mol / L.

[0069] 2) Preparation of DA-B-DEX aqueous solutions with different concentrations

[0070] The preparation concentration was 1×10 -4 , 1×10 -3 , 1×10 -2 , 1×10 -1 , 1, 10, 100, 1000mg / ml DA-B-DEX solution.

[0071] 3) Preparation of pyrene-containing micelles

[0072] Take 150 μl of the prepared pyrene solution, put it into several 10ml volumetric flasks, and evaporate the methanol to dryness. Then, add 10ml of DA-B-DEX solution of each concentration into the 10ml volumetric flask containing a trace amount of pyrene. Sonicate in a w...

Embodiment 3

[0076] A method for preparing a polymer micelle drug carrier with hydrogen peroxide responsiveness, the steps are as follows:

[0077] 1) DA-B-DEX (100mg) was dissolved in DMSO (10ml), and added dropwise into PBS buffer (100ml, pH7.4) under stirring, and kept stirring for 30min. Spontaneous formation of micelles was confirmed by observation of a clear opalescent solution.

[0078] 2) Simultaneously prepare DA-B-DEX micelles that physically entrap doxorubicin, DA-B-DEX (91.5mg) and DOX (8.5mg) are dissolved in 10mL dimethyl sulfoxide (DMSO) together, under stirring Add dropwise to 100mL PBS buffer, and keep stirring for 30min. Dialyze through a dialysis bag (molecular weight cut-off = 3.5KD) for 24 hours to remove organic solvents and free drugs, and finally freeze-dry and store.

[0079] 3) The particle size distribution is measured by using a Malvern laser particle size analyzer (Zetasized Nano, ZS, Malvern, United Kingdom). The morphology and size of micelles were evaluat...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a glucan polymer, a polymer micelle and a medicine carrier system. The glucan polymer is prepared from glucan, 4-(hydroxymethyl)benzeneboronic acid pinacol ester and polyol fatty acid through reaction, wherein the glucan and the polyol fatty acid provide hydrophilic and hydrophobic units, so that the polymer can automatically form the micelle; the polymer formed by the glucan, the 4-(hydroxymethyl)benzeneboronic acid pinacol ester and the polyol fatty acid through chemical reaction is sensitive to hydrogen peroxide; the response can be fast generated on hydrogen peroxide in an environment of ROS high expression diseases (such as myocardial ischemial injury, atherosclerosis, intestinal inflammation and tumor); the micelle disassembly is caused, so that the medicinemolecules are fast released.

Description

technical field [0001] The invention relates to the field of drug carriers, in particular to a dextran polymer, a polymer micelle and a drug carrier system. Background technique [0002] According to a research report recently released by the International Cancer Research Center of the World Health Organization, according to the current trend of cancer incidence, the incidence of cancer worldwide will increase by 50% in 2020, and the number of new cancer patients worldwide will reach 15 million each year. As if, cancer has become one of the common causes of morbidity and death today, but there is a lack of safe and effective treatments. [0003] At present, the main methods for treating cancer include surgery, chemotherapy, radiotherapy, targeted therapy, and immunotherapy. Chemotherapy is the abbreviation of chemical drug therapy, which achieves the purpose of treatment by using chemotherapy drugs to kill cancer cells. However, the selectivity of chemotherapy is low, the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/02A61K9/107A61K47/36A61K47/61A61K45/00A61P35/00A61P9/00A61P29/00
CPCA61K9/1075A61K45/00A61K47/36C08B37/0009C08B37/0024
Inventor 褚茂平孙昌正徐逸曾静静金其可卢孔场荣星林振坤李磊
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com