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Method for producing monosaccharide from edible mushroom bran

A technology for edible fungi and monosaccharides, applied in the field of monosaccharide production, can solve the problems of difficult separation of xylose and glucose, difficult industrial production, low hemicellulose content, etc., and achieve large-scale industrial production with mild process conditions. , the effect of efficient processing

Inactive Publication Date: 2018-05-22
CHANGAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention will provide a kind of method that utilizes edible fungus chaff to produce monosaccharide, to overcome the low hemicellulose content existing in the prior art, xylose and glucose are difficult to separate, the problem that is difficult to carry out industrialized production

Method used

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  • Method for producing monosaccharide from edible mushroom bran

Examples

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Embodiment 1

[0019] Step 1, acid hydrolysis: air-dry and crush the bran of various edible fungi such as Pingru, Coprinus comatus, and Golden Needle to about 10 mesh. ℃ for 30 minutes, after filtration, the filtrate and liquid residue were obtained;

[0020] The content of xylose in the measured filtrate is about 13g / L, and the conversion rate of hemicellulose can reach more than 92%. The filtrate is neutralized, concentrated, decolorized, ion-exchanged and crystallized to prepare food-grade xylose. The xylose output can reach 100g / kg fungus chaff, and further products such as xylitol and furfural can be produced.

[0021] Step 2, alkali hydrolysis: add the filter residue to a sodium hydroxide solution with a mass concentration of 5% at a solid-to-liquid ratio of 1:10, treat at 60°C for 60 minutes, neutralize with acid to neutral, and measure the cellulose content;

[0022] Step 3, enzymatic hydrolysis: add commercial cellulase ( CTec2, produced by Danish Novozymes Enzyme Preparation Com...

Embodiment 2

[0024] Step 1, acid hydrolysis: air-dry and crush the bran of various edible fungi such as Pingru, Coprinus comatus, and Flammulina to about 10 meshes, and add them to a sulfuric acid solution with a mass concentration of 2% according to a solid-to-liquid ratio of 1:15, 120 ℃ for 60 minutes, after filtration, the filtrate and liquid residue were obtained; the content of xylose in the filtrate was determined to be about 9g / L, and the conversion rate of hemicellulose could reach 90%. The filtrate prepares food-grade xylose through neutralization, concentration, decolorization, ion exchange and crystallization, and the output of xylose can reach 98g / kg bacterium chaff.

[0025] Step 2, alkali hydrolysis: add the filter residue to a sodium hydroxide solution with a mass concentration of 2% at a solid-to-liquid ratio of 1:15, treat at 60°C for 100 minutes, neutralize with acid to neutral, and measure the cellulose content;

[0026] Step 3, enzymatic hydrolysis: add commercial cellu...

Embodiment 3

[0028] Step 1, acid hydrolysis: air-dry and crush the bran of various edible fungi such as Pingru, Coprinus comatus, and Golden Needle to about 10 mesh. ℃ treatment for 120min, after filtration, the filtrate and liquid residue were obtained;

[0029] The content of xylose in the measured filtrate is about 7g / L, and the conversion rate of hemicellulose can reach 89%. The filtrate is neutralized, concentrated, decolorized, ion-exchanged and crystallized to prepare food-grade xylose, and the yield of xylose can reach 97g / kg fungus chaff.

[0030] Step 2, alkali hydrolysis: add the filter residue to a sodium hydroxide solution with a mass concentration of 5% at a solid-to-liquid ratio of 1:20, treat at 60°C for 60 minutes, neutralize with acid to neutral, and measure the cellulose content;

[0031] Step 3, enzymatic hydrolysis: add commercial cellulase ( CTec2, produced by Danish Novozymes Enzyme Preparation Company), the dosage is 15FPU / g cellulose, under the conditions of 50°...

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Abstract

The invention relates to the technical field of edible mushroom bran utilization and development, further relates to a method for producing monosaccharide from edible mushroom bran and aims to solve the problems that in the prior art the cellulose content is low, xylose is hard to separate from glucose, and industrial production is hard to implement. The method comprises the following steps: I, performing acid hydrolysis, namely drying edible mushroom bran in the air, crushing, adding a 1-5% diluted acid solution according to a solid-to-liquid ratio of 1:(10-20), treating for 30-120 minutes at100-120 DEG C, filtering so as to obtain filtrate and filter residues, and collecting food-grade xylose from xylose in the filtrate; II, performing alkali hydrolysis, namely adding a 1-5% alkali solution into the filtrate residues according to a solid-to-liquid ratio of 1:(10-20), treating for 60-120 minutes at 40-60 DEG C, performing acid neutralization till being neutral, and testing the content of cellulose; III, performing enzyme hydrolysis, namely adding cellulase according to the content of the cellulose, performing enzymolysis for 48-72 hours under the conditions of 50 DEG C and 180rpmso as to obtain an enzymolysis liquid, and filtering the enzymolysis liquid, thereby obtaining a glucose solution and lignin, wherein the amount of the cellulose is 10-20FPU / g.

Description

Technical field: [0001] The invention relates to the technical field of utilization and development of edible fungus chaff, and further relates to a method for producing monosaccharides from edible fungus chaff. technical background: [0002] Edible fungus bran is a solid medium that is discarded after cultivating edible fungi with corncobs, sawdust, cottonseed hulls, rice straw, crop straw or industrial waste (paper mill waste, vinegar grains, distiller's grains, etc.) as the main raw material. At present, the total output of edible fungi in my country has reached 4-5 million tons, with an output value of more than 30 billion yuan. The resulting fungus chaff quantity is also very huge, with an annual output of 7 to 9 million tons. At present, the overall utilization rate of the utilization method of fungus chaff is low, and the added value is not high, which has not only caused a large amount of waste of resources, but also polluted the environment. With the rapid develop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P19/02C08H7/00C13K13/00
CPCC12P19/14C08H6/00C12P19/02C13K1/02C13K13/002
Inventor 杨明琰兰萌高晓峰苗林平徐力
Owner CHANGAN UNIV
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