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Veterinary enrofloxacin solid lipid nanosuspension and preparation method thereof

A technology of solid lipid nanometer and enrofloxacin, which is applied in the directions of non-active ingredient medical preparations, active ingredients-containing medical preparations, pharmaceutical formulas, etc. Widespread clinical application and other issues, to achieve the effect of improving bioavailability, improving absorption, and reducing clinical doses

Active Publication Date: 2021-02-05
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have reported that myristic acid, palmitic acid and stearic acid solid lipid nanoparticles prepared by hot-melt emulsification ultrasonography can improve the in vivo absorption and circulation time of enrofloxacin in mice, but the three fatty acid solid lipid nanocapsules The sealing rate is lower than 70%, and the drug loading is lower than 7.3%, which limits the possibility of its wide clinical application (Xie et al., 2011)

Method used

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  • Veterinary enrofloxacin solid lipid nanosuspension and preparation method thereof
  • Veterinary enrofloxacin solid lipid nanosuspension and preparation method thereof
  • Veterinary enrofloxacin solid lipid nanosuspension and preparation method thereof

Examples

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Effect test

Embodiment 1

[0059] The formula of veterinary enrofloxacin solid lipid nanosuspension is shown in Table 1, and its preparation method is as follows:

[0060] (1) Weigh 12g of behenic acid and place it in a test tube heated by an oil bath at 120°C, add 2g of enrofloxacin bulk drug to it after heating and melting, and form a transparent enrofloxacin solution under constant stirring;

[0061] (2) Weigh 0.2g sodium lauryl sulfate and sodium bisulfite respectively and add them to 30mL of sterilized water to form a solution;

[0062] (3) Put the solution prepared in step (2) into the enrofloxacin solution prepared in step (1) after being heated in an oil bath at 120° C. at a stirring speed of 3000 to 5000 r / min to form an oil-in-water colostrum;

[0063] (4) Dispersing the colostrum prepared in step (3) with a disperser for 2 to 5 minutes to form an oil-in-water emulsion;

[0064] (5) Quickly pour the emulsion prepared in step (4) into 30 mL of sterilized water at 4°C to obtain the enrofloxaci...

Embodiment 2

[0070] The formula of veterinary enrofloxacin solid lipid nanosuspension is shown in table 2, and its preparation method is as follows:

[0071] (1) Weigh 12g of behenyl alcohol and place it in a test tube heated by an oil bath at 120°C, add 2g of enrofloxacin bulk drug to it after heating and melting, and form a transparent enrofloxacin solution under constant stirring;

[0072] (2) Weigh 0.5g poloxamer 188, 0.2g sodium formaldehyde sulfoxylate and 3.5g dimethyl dioctadecyl ammonium chloride respectively and add them to 30mL of sterilized water to form a solution;

[0073] (3) Put the solution prepared in step (2) into the enrofloxacin solution prepared in step (1) after being heated in an oil bath at 120° C. at a stirring speed of 3000 to 5000 r / min to form an oil-in-water colostrum;

[0074] (4) Dispersing the colostrum prepared in step (3) with a disperser for 2 to 5 minutes to form an oil-in-water emulsion;

[0075] (5) Quickly pour the emulsion prepared in step (4) int...

Embodiment 3

[0081] The formula of veterinary enrofloxacin solid lipid nanosuspension is shown in table 3, and its preparation method is as follows:

[0082] (1) Weigh 20g of behenyl alcohol and 15g of behenic acid respectively and place them in a test tube heated in an oil bath at 120°C. After heating and melting, add 6g of enrofloxacin raw material to it, and form transparent enrofloxacin under constant stirring. star solution;

[0083] (2) Weigh 2g polyvinylpyrrolidone K respectively 15 Add 1g of sodium formaldehyde sulfoxylate to 40mL of sterilized water to form a solution;

[0084] (3) Put the solution prepared in step (2) into the enrofloxacin solution prepared in step (1) after being heated in an oil bath at 120° C. at a stirring speed of 3000 to 5000 r / min to form an oil-in-water colostrum;

[0085] (4) Dispersing the colostrum prepared in step (3) with a disperser for 2 to 5 minutes to form an oil-in-water emulsion;

[0086] (5) Quickly pour the emulsion prepared in step (4) i...

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Abstract

The invention belongs to the technical field of veterinary medicine preparation. Specifically, it relates to a veterinary enrofloxacin solid lipid nanosuspension and a preparation method thereof. The invention utilizes the high-pressure homogeneous technology to carry out preparation process production. The components of the suspension injection by W / V include 12% to 35% of solid lipid matrix, 2% to 6% of enrofloxacin bulk drug, 0.2% to 4% of active agent, 0.2% to 1% of antioxidant, The balance is sterilized water. The solid lipid matrix adopted in the invention has good physiological compatibility, biodegradability, high nanoparticle encapsulation efficiency and drug loading capacity, simple preparation method, low production cost, and easy large-scale industrial production. The solid lipid nanosuspension of the present invention has an obvious slow-release effect, can increase the in vivo absorption and cellular uptake of active molecules, can reduce the number of clinical administration, is non-irritating to target animals, and can be used to prevent and control bacteria in pigs and chickens Gastrointestinal and respiratory diseases.

Description

technical field [0001] The invention belongs to the field of preparation of veterinary drug preparations, in particular to a veterinary enrofloxacin solid lipid nanosuspension and a preparation method thereof. Background technique [0002] Enrofloxacin is a fluoroquinolone antibacterial drug specially used for synthetic animals. It can achieve antibacterial effect by inhibiting bacterial DNA helicase. It has broad antibacterial spectrum, strong bactericidal activity, wide distribution in the body, and less toxic side effects. The drug has no cross-resistance and other characteristics, and is mainly used to treat bacterial infectious diseases in animals. Although enrofloxacin has strong antibacterial activity, its weak intracellular accumulation ability leads to limited clinical therapeutic effect on intracellular infection. The poor solubility and bitter taste of enrofloxacin lead to large changes in its absorption in animals, which affects the clinical therapeutic effect. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/10A61K31/496A61K47/10A61K47/12A61P31/04
CPCA61K9/0019A61K9/0053A61K9/10A61K31/496A61K47/10A61K47/12
Inventor 袁宗辉谢书宇杨飞陶燕飞瞿玮潘源虎陈冬梅黄玲利程古月刘振利谢长清
Owner HUAZHONG AGRI UNIV
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