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Thermo-sensitive western blot sensing microspheres based on quantum dots and preparation method of same

A technology of western blotting and quantum dots, applied in the fields of material science and bioseparation engineering, to achieve sensitive effects

Active Publication Date: 2018-04-27
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no record of western blot microspheres with both fluorescent sensing and thermosensitive response

Method used

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  • Thermo-sensitive western blot sensing microspheres based on quantum dots and preparation method of same
  • Thermo-sensitive western blot sensing microspheres based on quantum dots and preparation method of same
  • Thermo-sensitive western blot sensing microspheres based on quantum dots and preparation method of same

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preparation example Construction

[0029] The invention provides a method for preparing quantum dot-based temperature-sensitive western blot sensing microspheres, comprising the following steps:

[0030] 1) After mixing CdTe quantum dots co-modified with thioglycolic acid and glutathione, and a functional molecular protein solution, electrostatic adsorption is carried out to obtain an electrostatic adsorption solution;

[0031] 2) After mixing the electrostatic adsorption solution obtained in step 1), isopropylacrylamide, N,N-methylenebisacrylamide, potassium persulfate and water, carry out free radical polymerization to obtain a temperature based quantum dot Sensitive imprinted shell;

[0032] 3) eluting the temperature-sensitive imprinted shell obtained in step 2) with a mixed solution of absolute ethanol-sodium hydroxide to obtain temperature-sensitive western imprinted microspheres based on quantum dots.

[0033] The present invention mixes CdTe quantum dots co-modified with thioglycolic acid and glutathio...

Embodiment 1

[0070] Synthesis of CdTe quantum dots co-modified with thioglycolic acid and glutathione: Weigh 38.3mg of tellurium powder and 40mg of sodium borohydride into a 2mL yellow-cap bottle with a pointed bottom, first add 1.5mL of absolute ethanol, and then Add 0.5mL of deionized water, quickly cover the lid to make the system airtight, insert a needle on the lid, use deionized water on the needle to seal off oxygen, and react at a constant temperature of 40°C for 4 hours until the black tellurium powder is completely disappear until the supernatant is lavender. At the same time, add 92.4 mg of cadmium nitrate to 75 mL of deionized water, then add 63 μL of thioglycolic acid and glutathione, then adjust the pH to 9.2 with 1 mol / L sodium hydroxide solution, and blow with nitrogen for 20 minutes to remove oxygen. Add 1 mL of the supernatant of the tellurium powder product to the cadmium nitrate solution, and reflux for 2 hours under the protection of nitrogen to obtain yellow-green qua...

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Abstract

The invention provides a thermo-sensitive western blot sensing microsphere based on a quantum dot and a preparation method of same and belongs to the technical field of material science and bio-separation engineering. The method includes the steps of: 1) with a functional protein molecule as a template molecule, combining the functional protein molecule with CdTe quantum dots, which are co-modified with glutathione-thioglycollic acid (GSH-TGA), through electrostatic charge effect by means of amino groups and carboxyl groups, which are rich in the functional protein molecule; 2) in a non-oxygenenvironment, initiating polymerization by means of potassium persulfate (KPS), wherein thermo-sensitive isopropyl acrylamide (NIPAM) is used as a functional monomer and N,N-methylene bisacrylamide (MBA) is used as a crosslinker, so that through the free radical polymerization, the thermo-sensitive western blot sensing microsphere based on the quantum dot is produced. Through mechanism of electrontransfer between the functional protein and the quantum dot, fluorescent sensing of the microspheres to the template protein is achieved, and the synthesized western blot microspheres has performances such as thermo-sensitive response, fluorescent detection, etc.

Description

technical field [0001] The invention belongs to the technical field of material science and biological separation engineering, and in particular relates to a temperature-sensitive protein imprinting sensing microsphere based on quantum dots and a preparation method thereof. Background technique [0002] Molecular imprinting technology is a technology for preparing polymer compounds—molecularly imprinted polymers (MIPs) with specific predetermined selectivity for specific target molecules (also called template molecules, imprinted molecules). In today's era when various fields are moving closer to intelligence, smart imprinted materials, or stimuli-responsive molecularly imprinted materials, have also appeared in the field of molecular imprinting, that is, under certain signal stimuli, stimuli-responsive polymers can be stimulated by their own physical or chemical properties. To change the sensitive response to stimuli, the combination of stimuli-responsive polymer preparatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/54C08F222/38C08K9/04C08K3/30C08J9/26B01J20/26B01J20/30
CPCB01J20/268C08F220/54C08J9/26C08J2201/0424C08J2333/24C08K3/30C08K9/04C08F222/385
Inventor 李金花王晓艳陈令新王运庆杨倩傅骏青吴夏青
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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