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Liquid chromatography tandem mass spectrometry quantification method used for simultaneous detection of plurality of effective components in gelsemium elegans

An effective ingredient, liquid chromatography technology, applied in the field of Chinese herbal medicine analysis, to achieve the effect of effective separation

Active Publication Date: 2018-04-10
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Due to the limitation of its toxicity, Gelkins is not sold as any product in the market, but because of its effect of promoting animal growth, it is included in the 2015 edition of Veterinary Pharmacopoeia, so it has a certain wide application in veterinary clinic

Method used

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  • Liquid chromatography tandem mass spectrometry quantification method used for simultaneous detection of plurality of effective components in gelsemium elegans
  • Liquid chromatography tandem mass spectrometry quantification method used for simultaneous detection of plurality of effective components in gelsemium elegans
  • Liquid chromatography tandem mass spectrometry quantification method used for simultaneous detection of plurality of effective components in gelsemium elegans

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] Simultaneously detect the relative quantitative analysis method of 46 kinds of compounds in gecko, adopt high performance liquid chromatography triple quadrupole mass spectrometer to detect, described high performance liquid chromatography condition is: chromatographic column is Waters C18 chromatographic column, mobile phase A is 5mM acetic acid Ammonium (PH=5.2), the mobile phase B is acetonitrile, the flow rate is 0.3mL / min, the column temperature is 30°C, and the injection volume is 5 μL for gradient elution. The gradient elution program is: within 0-2min, the mobile phase The volume ratio of A and mobile phase B is 90:10, 2-7min, the volume ratio of mobile phase A and mobile phase B is 90:10 and changes to 85:15 at a constant speed; within 7-20min, mobile phase A and mobile phase B The volume ratio of mobile phase A and mobile phase B is 85:15, within 20-30min, the volume ratio of mobile phase A and mobile phase B is changed from 85:15 to 10:90 at a constant speed, ...

Embodiment 2

[0094] Simultaneously detect the relative quantitative analysis method of 46 kinds of compounds in gecko, adopt high performance liquid chromatography triple quadrupole mass spectrometer to detect, described high performance liquid chromatography condition is: chromatographic column is Waters C18 chromatographic column, mobile phase A is 5mM acetic acid Ammonium (PH=5.2), the mobile phase B is acetonitrile, the flow rate is 0.4mL / min, the column temperature is 25°C, the injection volume is 7μL, and gradient elution is carried out. The gradient elution procedure is the same as in case 1;

[0095] The mass spectrometry conditions are the same as in Example 1.

[0096] The solution preparation is the same as in Example 1.

[0097] Concrete steps are with embodiment 1.

Embodiment 3

[0099] Simultaneously detect the relative quantitative analysis method of 46 kinds of compounds in gecko, adopt high performance liquid chromatography triple quadrupole mass spectrometer to detect, described high performance liquid chromatography condition is: chromatographic column is Waters C18 chromatographic column, mobile phase A is 5mM acetic acid Ammonium (PH=5.2), the mobile phase B is acetonitrile, the flow rate is 0.5mL / min, the column temperature is 30°C, the injection volume is 10 μL, and gradient elution is carried out. The gradient elution procedure is the same as that of case 2;

[0100] The mass spectrometry conditions are the same as in Example 1.

[0101] Solution preparation is the same as embodiment 2.

[0102] Concrete steps are with embodiment 2.

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Abstract

The invention discloses a liquid chromatography tandem mass spectrometry quantification method used for simultaneous detection of a plurality of effective components in gelsemium elegans. According tothe liquid chromatography tandem mass spectrometry quantification method, a gelsemine methanol solution, a koumine methanol solution, a gelsenicine methanol solution, and a koumidine methanol solution are taken as reference sample solutions, a gelsemium elegans extracted solution is taken as a sample solution to be measured, a high performance liquid chromatography triple quadrupole mass spectrometer is adopted for detection, and the contents of the plurality of effective components in the sample solution to be measured are calculated based on detection results. The liquid chromatography tandem mass spectrometry quantification method is stable and reliable, is simple, is high in efficiency, sensitivity, and accuracy, possesses wide practicability, and can be used for detection and quantitative analysis of any components or derivative products from gelsemium elegans.

Description

technical field [0001] The invention relates to a relative quantitative analysis method, in particular to a relative quantitative analysis method for simultaneously detecting a plurality of compounds in geckos, and belongs to the technical field of Chinese herbal medicine analysis. Background technique [0002] Glycyrrhizae is the whole herb of Nutriaceae cucurbita. Glycyrrhizae mainly contains chemical components such as alkaloids, iridoids, phenolic acids, triterpenoids and oils. So far, domestic and foreign literature reports from A total of more than 300 compounds were isolated from Gelkinus plants. Gelkins has anti-tumor, anti-inflammatory, neuropathic pain and immune-enhancing effects, and is mainly used to treat rheumatoid arthritis pain, neuropathic pain, and promote animal growth. [0003] At present, it has been reported that more than 120 indole alkaloids have been isolated from Gelkins, which can be divided into sarpagines, koumines, humantenines, and methyl glu...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 刘兆颖刘艳纯杨昆孙志良
Owner HUNAN AGRICULTURAL UNIV
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