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Pseudomonas aeruginosa sanitizer applicable to production of barreled drinking water and sterilization method

A Pseudomonas aeruginosa and disinfectant technology, which is applied in the field of Pseudomonas aeruginosa disinfectant and sterilization, can solve the problems of unsatisfactory killing effect of Pseudomonas aeruginosa, achieve high safety and increase sterilization speed , the effect of high lethality

Active Publication Date: 2018-03-27
衡阳市食品药品检验检测中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the particularity of the product and the production environment, some compound disinfectants on the market (such as some disinfectants containing ethanol, chlorhexidine acetate, and disinfectants containing compound Chinese herbal plant components) are not suitable for the production process of bottled water Therefore, the above-mentioned single-component disinfectants with less than ideal killing effect on Pseudomonas aeruginosa are still widely used in bottled drinking water production plants

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Prepare the following components:

[0025] 1. Hydrogen peroxide aqueous solution with a concentration of 0.5g / L;

[0026] 2. CuSO with a concentration of 19mmol / L 4 aqueous solution;

[0027] Stir component 1 and component 2 according to the weight ratio of 8:1 and mix evenly. It is prepared and used immediately; then take 0.5ml with a concentration of about 10 6 CFU / ml of Pseudomonas aeruginosa bacteria liquid and the mixture of the above components 1 and 2 4.5mL, injected into a sterile test tube (in the mixed solution in the test tube, Cu 2+ Concentration of 1.9mmol / L, hydrogen peroxide concentration of 0.4g / L), act for 20 seconds, and immediately perform gradient dilution to an appropriate concentration; Bacterial concentration counting; after the culture medium solidified, flip the plate, culture at 36°C for 12 hours, count and calculate the lethality.

Embodiment 2

[0029] Prepare the following components:

[0030] 1. Hydrogen peroxide aqueous solution with a concentration of 0.9g / L;

[0031] 2. ZnSO with a concentration of 21mmol / L 4 aqueous solution;

[0032] Stir component 1 and component 2 according to the weight ratio of 8:1 and mix evenly. It is prepared and used immediately; then take 0.5ml with a concentration of about 10 6 CFU / ml of Pseudomonas aeruginosa bacteria liquid and the mixture of the above components 1 and 2 4.5mL, injected into a sterile test tube (in the mixed solution in the test tube, Zn 2+ Concentration of 2.1mmol / L, hydrogen peroxide concentration of 0.72g / L), act for 20 seconds, and immediately perform gradient dilution to an appropriate concentration; then take 1mL of the diluted solution and pour it on a plate, dilute the working bacterial solution with sterile physiological saline, and make Bacterial concentration counting; after the culture medium solidified, flip the plate, culture at 36°C for 12 hours, ...

Embodiment 3

[0034] Prepare the following components:

[0035] 1. Hydrogen peroxide aqueous solution with a concentration of 0.8g / L;

[0036] 2. FeCl with a concentration of 20mmol / L 3 aqueous solution;

[0037] Stir component 1 and component 2 according to the weight ratio of 8:1 and mix evenly. It is prepared and used immediately; then take 0.5ml with a concentration of about 10 6 CFU / ml of Pseudomonas aeruginosa bacteria liquid and the mixture of the above components 1 and 2 4.5mL, injected into a sterile test tube (in the mixed solution in the test tube, Fe 3+ Concentration of 2.0mmol / L, hydrogen peroxide concentration of 0.64g / L), act for 20 seconds, and immediately perform gradient dilution to an appropriate concentration; Bacterial concentration counting; after the culture medium solidified, flip the plate, culture at 36°C for 12 hours, count and calculate the lethal rate.

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Abstract

The invention discloses a pseudomonas aeruginosa sanitizer applicable to production of barreled drinking water and a sterilization method and relates to the technical field of sterilization and disinfection. The pseudomonas aeruginosa sanitizer comprises a main sterilization component, a sterilization accelerant and a solvent, wherein the main sterilization component is selected from one of hydrogen peroxide and chlorine dioxide; if the hydrogen peroxide is adopted as the main sterilization component, the concentration of the hydrogen peroxide in the pseudomonas aeruginosa sanitizer is 0.4-0.72g / L; when the chlorine dioxide is adopted as the main sterilization component, the concentration of the chlorine dioxide in the pseudomonas aeruginosa sanitizer is 0.192-0.48mg / L; the sterilization accelerant is one or a mixture of more than any two of FeCl3, CuSO4 and ZnSO4; the concentration of the sterilization accelerant in the pseudomonas aeruginosa sanitizer is 1.9-2.1mmol / L; the solvent iswater; and the pseudomonas aeruginosa sanitizer is a sanitizer which is prepared and used in situ. The sanitizer is high in security and high in fatality rate upon pseudomonas aeruginosa, and the sterilization speed of the sanitizer is greatly increased when being compared with those of conventional sanitizers of single components.

Description

technical field [0001] The invention relates to the technical field of disinfection and sterilization, in particular to a Pseudomonas aeruginosa disinfectant and a sterilization method suitable for the production of bottled drinking water. Background technique [0002] Pseudomonas aeruginosa is a common opportunistic pathogen that can cause a variety of inflammatory and intestinal diseases, and even death. This bacterium is widely distributed in nature. It exists in various water, air, normal human skin, respiratory tract and intestinal tract, etc., and has low nutritional requirements. The important condition for its existence is a humid environment. [0003] The production environment of barreled drinking water is humid, which is an ideal place for the growth of Pseudomonas aeruginosa. Pseudomonas aeruginosa can easily cause cross-contamination through water flow, personnel, packaging materials, etc., and eventually lead to unqualified products. In recent years, the pollu...

Claims

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Application Information

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IPC IPC(8): A01N59/00A01N59/20A01N59/16A01P1/00C02F1/50
CPCA01N59/00A01N59/16A01N59/20C02F1/50
Inventor 潘萍孔英戈汪华常怡虹王艳李俊歧周翔
Owner 衡阳市食品药品检验检测中心
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