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Saccharomycetes for degrading citrinin and application

A citrinin and yeast technology, applied in fungi, microorganism-based methods, microorganisms, etc., can solve problems such as low efficiency, affecting food sensory properties, and polluting the environment.

Inactive Publication Date: 2018-03-20
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many defects that affect the actual production and application: (1) affect the sensory properties of food
For example, changing the pH and adding some exogenous bactericidal substances will cause changes in the original color, fragrance and affect the sales of food
The use of various pesticides and other fungicides in chemical methods will cause many serious consequences, such as drug residues, resistance, environmental pollution, etc.
(3) The cost of equipment is high
For example, the methods of irradiation and cold storage are very effective, but the cost of equipment is high, and it is difficult to promote comprehensive use
(4) The application has certain limitations
For example, some cold processed products are not suitable for high temperature treatment if vegetable preservation, etc.
(5) Low efficiency
[0005] With the development of biological detoxification technology, the use of microorganisms to control the contamination of citrinin in food and feed products shows a good application prospect, but the relevant research is still in its infancy in the international scope, which affects the development of biological control technology in citrinin. applications in element control

Method used

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  • Saccharomycetes for degrading citrinin and application
  • Saccharomycetes for degrading citrinin and application
  • Saccharomycetes for degrading citrinin and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: the screening of degrading citrinin yeast, concrete implementation steps are as follows:

[0024] 1. Isolation of yeast single colonies from the environment

[0025] Take 1g of vineyard soil, add it into a centrifuge tube filled with 10mL sterile water, shake and mix well, take the mixed solution, and do gradient dilution (10 -4 , 10 -5 , 10 -6 , 10 -7 ), spread on NYDA medium plate, and culture at 28°C for 20-36h. Pick the dominant yeast single colony and purify and culture it on the NYDA plate. The isolated and purified strains were inoculated on NYDA slant, cultured at 28°C for 48 hours, and stored in a refrigerator at 4°C for short-term storage.

[0026] 2. Determination method of citrinin

[0027] Adopt the method (HPLC-FLD) of high performance liquid phase-fluorescence detector to detect citrinin, the condition is as follows, chromatographic column: Agilent C 18 Column (5.0μm, 150.0mm×4.6mm); mobile phase: acetonitrile: water (0.03% phosphoric...

Embodiment 2

[0035] Example 2: Microbiological properties of Cryptococcus Y3

[0036] The above-mentioned strain Y3 was identified as Cryptococcus podzolicus through morphological culture, physiological and biochemical characteristics tests, and nucleotide sequence analysis of the small subunit 5.8S rDNA and the internal transcribed spacer ITS1 and ITS2 regions.

[0037] Bacterial strain C.podzolicus Y3 bacterial strain (bacterial strain number: CCTCC NO:M2017505) of the present invention has the following microbiological characteristics:

[0038] 1. Morphological features

[0039] (1) On NYDA solid medium plate (beef extract 8g, yeast extract 5g, glucose 10g, agar 20g, distilled water 1000mL; after dispensing into Erlenmeyer flasks, sterilize at 121°C for 20min.) Cultivate at 28°C for 48h, the colonies are eggs Round shape, neat edges, milky white, smooth surface, uniform texture, easy to pick.

[0040] (2) After culturing in NYDB liquid medium for 24 hours, no mold was formed, the bact...

Embodiment 3

[0044] Example 3: Optimization of Citrinin Degradation Conditions by Cryptococcus Y3

[0045] By studying the effect of Cryptococcus Y3 on the degradation of citrinin under the conditions of bacterial concentration, culture temperature, medium pH and different initial concentrations of citrinin, the optimal condition for its degradation of citrinin was determined to be the initial concentration of yeast for 10 8 cells / mL, pH 4, shaker culture temperature 28°C, rotation speed 180rpm, and with the increase of the initial concentration of citrinin, the degradation efficiency is accelerated. When the initial concentration of citrinin was 5 μg / mL, the yeast degradation rate was 77% after 42 hours; when the initial concentration of citrinin was 10 μg / mL, the yeast degradation rate was 96% after 42 hours; When the initial concentration was 20μg / mL, the degradation rate of yeast was 98% after 42h; within 21h-28h, the degradation efficiency was the highest.

[0046] The result shows ...

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Abstract

The invention discloses saccharomycetes for degrading citrinin and application and belongs to the technical field of biology. After being identified, the saccharomycetes is Cryptococcus podzolicus Y3,and a preservation strain number is CCTCC NO:M2017505. The optimal conditions for the Cryptococcus podzolicus Y3 to degrade the citrinin are that an initial concentration of the saccharomycetes is 108cells / mL, a pH is 4, a shake cultivation temperature is 28 DEG C, and a rotation speed is 180rpm. The saccharomycetes can degrade the citrinin in high efficiency, a degradation rate can reach 98%, and furthermore, the degradation rate of the saccharomycetes to the citrinin is the maximum within 21 to 35h.

Description

technical field [0001] The invention belongs to a method for biodegrading toxins, and in particular relates to the application of Cryptococcus podzolicus Y3 yeast in the fields of feed and food safety and the like. Background technique [0002] Citrinin (CIT) is a fungal secondary metabolite with nephrotoxicity. Its pure product is an acidic lemon yellow needle-like crystal with a molecular formula of C 13 h 14 o 5 , the structural formula is 5-methyl-6-oxo 3-2-benzoxazole-7-carboxyl, the melting point is 172°C, the polarity is low, but the fat solubility is high, and it is easily soluble in most organic solvents such as methanol and acetonitrile . Its special structure determines that CIT is toxic, and it is mainly produced by Penicillium, Aspergillus and Monascus. The toxicity test of CIT on PK15 cells (pig kidney cells) shows that when the concentration of CIT is greater than 50mol / L, the calcium balance of cells will be disturbed, resulting in the death of PK15. The...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16A23L5/20C12R1/645
CPCA23L5/28C12N1/16C12N1/145C12R2001/645
Inventor 张红印张晓云赵利娜郑香峰林珍任晓锋
Owner JIANGSU UNIV
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