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Method for the detection of six active ingredients in pharmaceutical samples

A technology for active ingredients and samples, applied in the field of medicine, can solve the problems such as the lack of public reports on the content method of active ingredients in cold medicines, and achieve the effects of wide range of detection applications, wide range of detection applications and simple operation.

Active Publication Date: 2020-08-04
HUMANWELL PURACAP PHARM WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no public report on the method of simultaneously determining the content of multiple drug active components in cold medicines

Method used

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  • Method for the detection of six active ingredients in pharmaceutical samples
  • Method for the detection of six active ingredients in pharmaceutical samples
  • Method for the detection of six active ingredients in pharmaceutical samples

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] In this embodiment, according to the configuration method and test conditions basically the same as the general method, 20 microliters of the reference substance solution was taken and injected into a high-performance liquid chromatograph for detection. The difference is that the mobile phase ratio used in this embodiment carries out gradient elution according to the following conditions:

[0047]

[0048]

[0049] The chromatogram that this embodiment detects obtains is as figure 1 shown. Depend on figure 1 The relevant information of each chromatographic peak can be obtained, as follows:

[0050] active ingredient name retention time (min) relative retention time Separation Phenylephrine Hydrochloride 4.17 0.38 - Acetaminophen 5.94 0.54 15.8 doxylamine succinate 7.10 0.65 13.0 Chlorpheniramine maleate 8.82 0.80 20.1 Guaifenesin 9.13 0.83 3.4 Dextromethorphan Hydrobromide 11.00 1.00 20.9

[00...

Embodiment 2

[0053] In this embodiment, 20 microliters of the sample solution was taken and injected into a high-performance liquid chromatograph for detection according to the test conditions basically the same as in the embodiment 1. The difference is that the drug sample used in this example is specifically DaytimeSevere Cold and Flu soft capsules, including acetaminophen components and guaiacol components.

[0054] The chromatogram of the sample solution of this embodiment is as figure 2 shown.

[0055] Depend on figure 2 It can be seen that the relevant information of the chromatographic peaks corresponding to the two components is shown in the following table:

[0056] active ingredient name retention time (min) relative retention time Acetaminophen 5.98 0.54 Guaifenesin 9.19 0.84

Embodiment 3

[0058] In this embodiment, 20 microliters of the sample solution was taken and injected into a high-performance liquid chromatograph for detection according to the test conditions basically the same as in the embodiment 1. The difference is that the samples used in this embodiment are specifically Nighttime Coldand Flu soft capsules, including doxylamine succinate components and dextromethorphan hydrobromide components.

[0059] The chromatogram of the sample solution of this embodiment is as image 3 shown.

[0060] Depend on image 3 It can be seen that the relevant information of the chromatographic peaks corresponding to the two components is shown in the following table:

[0061] active ingredient name retention time (min) relative retention time doxylamine succinate 7.20 0.65 Dextromethorphan Hydrobromide 11.06 1.00

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Abstract

The invention provides a method for six active components in a drug sample. In the method, the six active components comprise paracetamol, phenylephrine hydrochloride, doxylamine succinate, dextromethorphan hydrobromide, guaifenesin and chlorpheniramine maleate; the method uses high performance liquid chromatography (HPLC) to detect the drug sample, wherein the mobile phase of HPLC comprises a mobile phase A which is an aqueous solution containing trifluoroacetic acid of which the concentration is 0.1v / v% and a mobile phase B which is a mixed solution of acetonitrile and methanol in a volume ratio of 60: 40. The detection method provided by the invention can simply and quickly detect the six active components in the drug sample simultaneously, and the six active components can be separatedeffectively, and the detection method is simply operated, and quickly analyzed, suitable for detection of most cold medicines and wide in detection application range.

Description

technical field [0001] The invention relates to the field of medicine, in particular, the invention relates to a method for detecting six active ingredients in a drug sample. Background technique [0002] Cold, as a kind of modal respiratory disease, has many kinds of medicines for its treatment, among them, the safety transparency and quick effect of western medicine are favored by people. Most cold medicines are compound preparations, and the composition of Western medicine mainly includes the following types of drugs: antipyretic and analgesic drugs, adrenergic receptor agonists, antihistamines, antitussive and expectorant drugs, and so on. [0003] At present, there is no public report on a method for simultaneously determining the contents of multiple drug active components in cold medicines. Therefore, establishing a set of scientific and effective analytical methods that can simultaneously determine the content of main components in cold medicines has important appli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 徐欣秦巨波代蕾黄芸陈艳华晓维余爽
Owner HUMANWELL PURACAP PHARM WUHAN CO LTD
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