Aspergillus aculeatus p93 and its biological fertilizer preparation and application

A technology of Aspergillus aculeatus and P93, which is applied in the direction of fertilizers made from biological wastes, applications, microorganisms, etc. It can solve the problems of low utilization rate of phosphate fertilizers and decreased potential for increasing production, and achieve obvious effects, save phosphate fertilizer resources, and promote production and increase production. obvious effect

Active Publication Date: 2019-07-23
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0006] The present invention mainly aims at the problems of phosphorus deficiency, low utilization rate of phosphate fertilizer and decreased yield increase potential in my country, and provides a widely adaptable high-efficiency phosphorus-dissolving growth-promoting bacterium-Aspergillus aculeatus P93, microbial agent and biological fertilizer preparation prepared therefrom and its application

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  • Aspergillus aculeatus p93 and its biological fertilizer preparation and application
  • Aspergillus aculeatus p93 and its biological fertilizer preparation and application
  • Aspergillus aculeatus p93 and its biological fertilizer preparation and application

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preparation example Construction

[0035] The preparation of embodiment 1 Aspergillus aculeatus P93 microbial agent

[0036] 1. Isolation and purification of Aspergillus aculeatus P93

[0037]A bacterial strain P93 that can efficiently dissolve insoluble phosphorus was isolated and purified from farmland soil (calcareous soil) in Shijiazhuang City, Hebei Province.

[0038] According to the mycological characteristics of the strain P93 and the results of ITS rRNA gene sequencing, the strain P93 was identified as Aspergillus aculeatus.

[0039] 2. Cultivation of Aspergillus aculeatus P93

[0040] (1) Composition of medium (kg / t fermentation liquid)

[0041] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg,K 2 HPO 4 0.2kg, NaCl 2.5kg, MgSO 4 ·7H 2 O 0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0042] (2) Fermentation and cultivation process

[0043] Aspergillus aculeatus P93 was i...

Embodiment 2

[0048] Embodiment 2 Bacterial strain P93 solid plate test phosphorus dissolving effect

[0049] In order to clarify the ability of Aspergillus aculeatus P93 to dissolve insoluble phosphorus, tricalcium phosphate Ca was used in this example 3 (PO 4 ) 2 As a phosphorus source, cultivate at 28°C for 5 days, and measure the available phosphorus on the PDA plate. Penicillium bayley ATCC 20851 and Penicillium oxalicum P8 (CGMCC 2272) were used as control strains in the test, and a control test without inoculation was set up.

[0050] The results show that P93 fungus has the strongest ability to dissolve tricalcium phosphate, and the dissolved phosphorus is as high as 48.16mg phosphorus / plate, which is 128.8% higher than the dissolved phosphorus (21.05mg phosphorus / plate) of bacterial strain ATCC 20851 (table 1); the dissolved phosphorus of bacterial strain P93 Slightly lower than Penicillium oxalicum P8, the amount of dissolved phosphorus is 48.76mg phosphorus / plate. Colonies fo...

Embodiment 3

[0054] The effect of embodiment 3 phosphate-dissolving bacterial strain P93 dissolving insoluble phosphorus in liquid medium

[0055] In liquid culture medium at 20°C, 17rpm, after 5 days of cultivation, the dissolution effect of phosphate-dissolving fungus P93 on tricalcium phosphate, aluminum phosphate, iron phosphate, zinc phosphate and phosphate rock powder was measured. The results showed that the Aspergillus aculeatus P93 strain released the highest available phosphorus from tricalcium phosphate, reaching 934.89 μg phosphorus / mL, accounting for 94.2% of the total phosphorus added; the strain P93 had a strong ability to dissolve aluminum phosphate, and the available phosphorus was 494.73 μg phosphorus / mL, respectively accounted for 39.0% of the total phosphorus added; strain P93 has a strong ability to dissolve iron phosphate, and the released available phosphorus also reached 344.6 μg phosphorus / mL, accounting for 33.6% of the added insoluble phosphorus; strain P93 dissol...

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Abstract

The invention discloses aspergillus aculeatus P93 and the biological fertilizer preparation and application thereof. The deposit number of Aspergillus aculeatus P93 is CGMCC NO.14150. The inoculum prepared by Aspergillus aculeatus P93 has obvious effects on promoting crop growth and yield and activating soil phosphorus. Bacteria agent P93 can dissolve a variety of insoluble phosphorus. It can efficiently dissolve phosphate rock powder, iron phosphate, zinc phosphate and tricalcium phosphate in liquid medium, with a dissolution rate of 12.1-94.2%. Under soil conditions, five kinds of insoluble phosphorus sources are used After inoculation with fungal agent P93, soil available phosphorus increased by 257.8-1004.6%; inoculation of fungal agent P93 promoted corn phosphorus uptake, and total phosphorus of corn plants increased by 3.4-136.4%; the phosphorus-dissolving ability exceeded the currently reported internationally recognized P. (ATCC 20851). The bacterial agent is of great significance to the saving of phosphate fertilizer resources in my country's agricultural production, the continuous increase of grain production and the protection of the ecological environment of farmland.

Description

technical field [0001] The invention relates to the field of microbial preparations, in particular to Aspergillus aculeatus P93 and biological fertilizer preparations and applications prepared therefrom. Background technique [0002] Phosphorus is the second most important element for plant growth after nitrogen, but its availability is very low due to soil fixation, making it difficult to meet the needs of crop growth (Wild, 1988). Studies have shown that more than 90% of the phosphorus fertilizer applied to the soil is converted into insoluble phosphorus, acidic soil is converted into iron phosphate and aluminum phosphate, and calcareous soil is converted into calcium phosphate (Hagin and Hadas, 1962; Nagelschmidt G, et al. 1994). In order to increase crop yield, the amount of phosphate fertilizer has increased sharply, and phosphate rock resources are facing a crisis of depletion. It is estimated that the world's phosphate rock can be used for 60-90 years (Runge-Metzger ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C05G3/00C12R1/66
CPCC05B7/00C05D3/02C05G3/00C12N1/14C12N1/145C12R2001/66C05D9/00C05F5/002C05F11/00C05F11/08C05C9/00
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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