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Application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant

A technology of bimetallic nanoparticles and gold nanoparticles is applied in the directions of heavy metal active ingredients, medical preparations with non-active ingredients, medical raw materials derived from mammals, etc.

Inactive Publication Date: 2018-02-02
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention provides nano-particles with catalytic activity of catalase as cell immunotherapy promoter, combined with cell immunotherapy to improve tumor killing ability, but there is no similar report in relevant literature and patents at home and abroad

Method used

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  • Application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant
  • Application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant
  • Application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1 Preparation of gold-platinum bimetallic nanoparticles:

[0020] Heat 50 mL of chloroauric acid aqueous solution with a mass fraction of 0.01% to boiling and reflux, and quickly add 1 mL of 0.068M sodium citrate aqueous solution under high-speed stirring. Stir and cool to room temperature to obtain a gold nanoparticle sol with a diameter of about 13 nm; then add 150 μL of 50 mM chloroplatinic acid solution, heat and stir at 80 °C for 10 min, then slowly add 800 μL of 10 mM ascorbic acid, continue heating and stirring for 30 min, then stir and cool to At room temperature, gold-platinum nanoparticles with a diameter of about 18 nm are obtained; take 5 mL of gold-platinum nano-particle solution, add 0.05 mg of mercapto polyethylene glycol, shake the reaction for 1 hour, wash twice with deionized water, and obtain polyethylene glycol Modified gold-platinum bimetallic nanoparticles.

[0021] figure 1 It is the TEM picture of the gold-platinum bimetallic nanopar...

Embodiment 2

[0022] Example 2 Using gold-platinum bimetallic nanoparticles to catalyze the production of oxygen from hydrogen peroxide in the microenvironment of hypoxic tumor cells:

[0023] Human ovarian cancer cell line SKOV3 cells were digested with trypsin and suspended in cell culture medium. Through mixed gas (94%N 2 +1%O 2 +5% CO 2 ) for 30 minutes to make the cells hypoxic, then add 1.2 nM gold-platinum bimetallic nanoparticles, and immediately use a dissolved oxygen meter to detect the change of oxygen content in the solution over time. The experimental results are shown in figure 2 .

[0024] Depend on figure 2 It can be seen that a significant increase in oxygen content can be detected within 5 min of adding nanoparticles.

Embodiment 3

[0025] Example 3 Investigating the killing effect of gold-platinum bimetallic nanoparticles combined with CIK cells on hypoxic tumor cells

[0026] The killing ability mediated by CIK cells was detected by CCK-8 method.

[0027] The human ovarian cancer cell line SKOV3 cells were used as target cells and seeded in 96-well plates (5×10 3 pieces / hole). After the cells adhered to the wall, the first group of normoxia (21% O 2 ) culture, the second group was hypoxic (1% O 2 ) environment culture, the third group was hypoxic (1% O 2 ) environment and adding gold-platinum bimetallic nanoparticles, 5 wells in parallel for each group. The three groups were cultured at 37°C for 16h. CIK cells were used as effector cells, inoculated according to the effect-to-target ratio (E:T) of 10:1, 5:1, 3:1, and 1:1. After 4 hours of culture, 10% CCK-8 solution was added to each well and reacted for 4 hours. , detect the absorbance (A) value of each well at a wavelength of 450nm on a micropla...

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Abstract

The invention discloses an application of gold platinum thermometal nano particles on preparation of cell immunotherapy accelerant. According to the application, the hydrogen peroxide enzymatic activity of the gold platinum thermometal nano particles is used, the gold platinum thermometal nano particles can react with hydrogen peroxide in the tumour hypoxia microenvironment and generate oxygen andhave good biocompatibility, the gold platinum thermometal nano particles are combined with cell immunotherapy and used as accelerant of cell immunotherapy, so that the tumour hypoxia situation is improved, inhibition of the tumour hypoxia environment to the killing capacity of the immune cell is lowered, accordingly, the killing effect of the immune cell to the hypoxia tumour cell is remarkably improved, and the gold platinum thermometal nano particles have wide application prospects on tumour immunotherapy.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to the application of a gold-platinum bimetallic nanoparticle in the preparation of a cell immunotherapy accelerator. Background technique [0002] Adoptive immunotherapy holds great promise for many cancer immunotherapies. Among them, adoptive cytokine-induced killer cell (CIK cell) therapy is considered to be the first choice for a new generation of anti-tumor adoptive cell immunotherapy. CIK cells are a group of heterogeneous cells obtained after co-cultivating human peripheral blood mononuclear cells with various cytokines (such as anti-CD3 monoclonal antibody, interleukin-2 and interferon-γ) for a period of time in vitro. Because this kind of cell expresses both CD3+ and CD56+ membrane protein molecules, it is also called NK cell-like T lymphocyte, which has both the strong anti-tumor activity of T lymphocytes and the non-major histocompatibility of natural killer cells Co...

Claims

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Application Information

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IPC IPC(8): A61K33/24A61K35/17A61K47/69A61K47/60A61P35/00
CPCA61K33/24A61K35/17A61K2300/00
Inventor 李娟梁虹杨黄浩吴颖
Owner FUZHOU UNIV
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