Two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH) and preparation method of kit

Abstract

The invention discloses a two-photon fluorescence immunochromatography kit for quantitative determination of anti-Mullerian hormone (AMH), which utilizes fluorescent dye as a maker. The two-photon fluorescence immunochromatography kit, realizing fluorescence immunochromatography quantitative determination, has the advantages of good stability, wide linear range, good specificity, high sensitivity,high quantitative accuracy and easy and quick operation, can be applied to detection of whole blood samples, serum samples and plasma samples simultaneously, and is applicable to medical treatment ofhospital at different levels and family practice.

Description

Technical field [0001] The invention relates to the field of cancer clinical immunodiagnostic kits in the field of medical testing, in particular to a two-photon fluorescence immunochromatographic kit for quantitative detection of anti-Müllerian hormone (AMH). Background technique [0002] In the past 20 years, assisted reproductive technology (ART) has continuously improved, and the success rate has increased significantly, but ovarian reserve is still the main factor affecting the success rate of in vitro fertilization and embryo transfer (IVF-ET) factor. A large number of studies have found that anti-Mullerian hormone (AMH) can more accurately assess the reserve function of the ovary and predict the responsiveness of the ovaries to gonadotropins than traditional indicators. It has even found that the ability of follicles to synthesize AMH is related to the ability of the egg and the egg. The quality of embryos is related. It is believed that AMH can predict the outcome of ART...

AI Technical Summary

Problems solved by technology

[0009] At present, the immunological methods commonly used in clinical AMH detection include enzyme-linked immunoassay, chemiluminescence, and electrochemiluminescence; because the enzyme-linked immunoassay requires enzyme labeling, the process is complicated, and professional personnel are required to operate, and the American Union immunoassay Liquid reagents require low-temperature refrigerated transportation, which brings great inconvenience to grassroots medical institutions; both tube chemiluminescence and electrochemiluminescence require large-scale equipment, and are closed systems, which are expensive; some people use time resolution Fluorescence method, the emission wavelength of this method is usually around 300nm, there are a large number of protein molecules in blood samples, protein molecules have strong absorption in ultraviolet light, resulting in reduced sensitivity, etc.

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