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Cationic liposome influenza vaccine capable of entrapping quantum dots and preparation method thereof

A cationic liposome and influenza vaccine technology, which is applied in the field of novel particle drug delivery system, cationic liposome influenza vaccine and its preparation, can solve the problems of poor patient compliance, single route of vaccination, weak immunogenicity, etc. Toxic side effects, improved fluorescence stability, high luminous intensity effects

Active Publication Date: 2018-01-16
NINGXIA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to provide a cationic liposome influenza vaccine capable of carrying quantum dots, so as to overcome the defects of weak immunogenicity, single vaccination route, and poor patient compliance in current influenza vaccines, so that it can pass through the nasal mucosa After immunization, it can effectively induce humoral and mucosal immune responses, and enhance the immune response effect. Through in vitro cell uptake and stimulation maturation experiments, the immunological mechanism is preliminarily explored

Method used

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  • Cationic liposome influenza vaccine capable of entrapping quantum dots and preparation method thereof
  • Cationic liposome influenza vaccine capable of entrapping quantum dots and preparation method thereof
  • Cationic liposome influenza vaccine capable of entrapping quantum dots and preparation method thereof

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Effect test

Embodiment 1

[0054] Embodiment 1: film dispersion method prepares DDA-TDB liposome influenza vaccine

[0055] Put DDA and TDB in a 50 ml round bottom flask with a molar ratio of 7 to 10:1, add 1 to 2 ml of chloroform and methanol to dissolve, add quantum dots, and remove the organic solvent by rotary evaporation under reduced pressure under heating in a water bath to form a uniform Lipid film, and then pass through 3minN 2 , remove the residual solvent. Then 2 ml of 10 mM Tris-HCl buffer (pH 6.8-9) was added, ultrasonicated in a water bath at 60°C and incubated for 45 min to obtain blank liposomes with a particle size of about 600-700 nm. Then, according to the influenza vaccine: DDA-TDB mass ratio of 10-12.5:100, the influenza vaccine stock solution was added to the above-mentioned blank liposomes to make them fully fused, and stored at 4°C for future use. The particle size of the cationic liposome influenza vaccine obtained above is 1700-1900nm, and compared with the blank liposome, th...

Embodiment 2

[0056] Embodiment 2: film dispersion method prepares DOTAP-DC-Chol liposome influenza vaccine

[0057] Put DOTAP and DC-Chol in a 1:1 molar ratio in a 50 ml round bottom flask, add 1-2 ml

[0058] Dissolve chloroform and methanol, add quantum dots, and remove the organic solvent by rotary evaporation under reduced pressure under water bath heating to form a uniform lipid film, and then pass through 3minN 2 , remove the residual solvent. Then add 2 ml of 10 mM Tris-HCl buffer solution (pH 6.8-7.4), sonicate in a water bath at 60°C and incubate for 30 min to obtain blank liposomes with a particle size of about 250-270 nm. Then, according to the mass ratio of influenza vaccine:DOTAP-DC-Chol of 5-12.5:100, the stock solution of influenza vaccine was added to the above-mentioned blank liposomes to make them fully fused, and stored at 4°C for future use. The cationic liposome influenza vaccine obtained above has a particle size of 330-350nm, a zeta of 49-54mV, an encapsulation eff...

Embodiment 3

[0059] Embodiment 3: film dispersion method prepares DOTAP-DC-Chol liposome influenza vaccine

[0060] Put DOTAP and DC-Chol in a 1:1 molar ratio in a 50 ml round bottom flask, add 1-2 ml

[0061] Dissolve in chloroform and methanol, and remove the organic solvent by rotary evaporation under reduced pressure under heating in a water bath at 35-40°C to form a uniform lipid film, and then pass it through for 3minN 2 , remove the residual solvent. Then add 2 ml of 10 mM Tris-HCl buffer (pH 6.8-7.4), sonicate in a water bath at 55-65°C and incubate for 30 min to obtain blank liposomes with a particle size of about 230-290 nm. Then, according to the mass ratio of influenza vaccine: DOTAP-DC-Chol of 5-12.5:100, the stock solution of influenza vaccine was added to the above blank liposome, and quantum dots were added to make it fully fused, and stored at 4°C for future use. The cationic liposome influenza vaccine obtained above has a particle size of 310-370nm, a zeta of 45-57mV, a...

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Abstract

The invention relates to a cationic liposome influenza vaccine capable of entrapping quantum dots and a preparation method thereof. The cationic liposome influenza vaccine adopts a cationic complex asa vaccine adjuvant and a carrier, and is prepared by entrapping the quantum dots and influenza vaccine stock into the cationic liposome complex by adopting a film scattering method, a freeze-thaw method or a freeze drying method; and the particle size of the obtained cationic liposome influenza vaccine is between 100nm and 3 micrometers, a zeta potential is 30 to 90 mV, the entrapping rate is 45percent to 95 percent, and the drug carrying capacity is 2 to 10 percent. The cationic liposome influenza vaccine entrapping the quantum dots prepared by the invention can be used as a biological probe to capture and image cells and is good in stability; and by virtue of the immunization of mouse nasal mucosa, the novel vaccine can significantly improve the mouse humoral immunity and mucosal immunity response levels.

Description

technical field [0001] The invention belongs to a novel microparticle drug delivery system in the technical field of nanomaterials, and in particular relates to a cationic liposome influenza vaccine capable of encapsulating quantum dots and a preparation method thereof. Background technique [0002] Vaccine is an antigen preparation used to prevent infectious diseases. In recent years, with the continuous deepening of immunology technology and the rapid development of genetic engineering technology, new vaccines such as recombinant DNA vaccines and synthetic peptide vaccines have emerged continuously. Compared with these new vaccines, they have high purity and strong specificity, but small molecules and weak immunogenicity make it difficult to induce an effective immune response in the body. Therefore, adjuvants are needed to enhance their immunogenicity or enhance the host's protective response to antigens. [0003] Liposome is an adjuvant that has a strong synergistic effe...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K39/145A61K39/39A61P31/16
Inventor 杨建宏于蕊渠文静李娜左文宝侯延辉刘艳华
Owner NINGXIA MEDICAL UNIV
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