Green production method of L-aspartic acid

A technology of aspartic acid and ammonium aspartate, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of high comprehensive cost, difficult separation, high cost, etc.

Inactive Publication Date: 2017-12-15
ANHUI XUELANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the molar yield of L-aspartic acid product is only about 85%, and the overall cost is high
[0006] Preparation of L-aspartic acid by chemical synthesis, fumaric acid is added with ammonia to obtain DL-aspartic acid isomers under high temperature and pressure, which is difficult to separate and expensive

Method used

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  • Green production method of L-aspartic acid
  • Green production method of L-aspartic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for preparing L-aspartic acid, such as figure 1 shown, including the following steps:

[0026] 1) maleic acid reacts with ammonia to obtain ammonium maleate;

[0027] 2) Enzyme isomerization

[0028] 2.1) The maleate isomerase and L-aspartate transaminase genes are transferred into microbial Escherichia coli for fermentation, and the fermentation broth contains 0.02% magnesium sulfate, 0.1% potassium dihydrogen phosphate, 0.2 % sodium chloride, 1.5% corn steep liquor, the pH of the culture solution is 6-7, the fermentation temperature is 35°C, the OD value is 0.2-0.3, and the culture time is 15 hours, the enzyme liquid containing two kinds of enzymes is obtained ;

[0029] 2.2) Stirring the ammonium maleate obtained in step 1) and the enzyme solution obtained in step 2.1) at 30° C. for an enzyme reaction to obtain an ammonium L-aspartate feed solution;

[0030] 3) Add 0.5% activated carbon to the L-aspartic acid ammonium feed solution obtained in step 2.2) ...

Embodiment 2

[0042] A method for preparing L-aspartic acid, such as figure 1 shown, including the following steps:

[0043] 1) maleic acid reacts with ammonia to obtain ammonium maleate;

[0044] 2) Enzyme isomerization

[0045] 2.1) Maleate isomerase and L-aspartate transaminase genes are transferred into microbial Escherichia coli for fermentation, and the fermentation broth contains 0.03% magnesium sulfate, 0.05% potassium dihydrogen phosphate, 0.3 % sodium chloride, 1.2% corn steep liquor, the pH of the culture solution is 6-7, the fermentation temperature is 36°C, the OD value is 0.3-0.4, and the culture time is 18 hours, the enzyme liquid containing two kinds of enzymes is obtained ;

[0046] 2.2) Stirring the ammonium maleate obtained in step 1) and the enzyme solution obtained in step 2.1) at 35° C. for an enzyme reaction to obtain an ammonium L-aspartate feed solution;

[0047] 3) Add 0.75% activated carbon to the ammonium L-aspartate feed solution obtained in step 2.2) to decol...

Embodiment 3

[0052] A method for preparing L-aspartic acid, such as figure 1 shown, including the following steps:

[0053] 1) maleic acid reacts with ammonia to obtain ammonium maleate;

[0054] 2) Enzyme isomerization

[0055] 2.1) Maleate isomerase and L-aspartate transaminase genes are transferred into microbial Escherichia coli for fermentation, and the fermentation broth contains 0.01% magnesium sulfate, 0.2% potassium dihydrogen phosphate, 0.4 % sodium chloride, 1.5% corn steep liquor, the pH of the culture solution is 6-7, the fermentation temperature is 37°C, the OD value is 0.2-0.3, and the culture time is 16 hours, the enzyme solution containing two enzymes is obtained ;

[0056] 2.2) Stirring the ammonium maleate obtained in step 1) and the enzyme solution obtained in step 2.1) at 38° C. for an enzyme reaction to obtain ammonium L-aspartate feed solution;

[0057] 3) Add 0.5% activated carbon to the ammonium L-aspartate feed solution obtained in step 2.2) for decolorization...

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Abstract

The invention provides a method for preparing L-aspartic acid. The method comprises the following steps: enabling maleic acid and ammonia to react to obtain ammonium maleate; stirring an enzyme solution obtained by fermenting the obtained ammonium maleate and microorganisms at 30 DEG C to 40 DEG C and carrying out enzyme reaction, so as to obtain an ammonium L-aspartate material solution, wherein the enzyme solution obtained by microorganism fermentation is an enzyme solution which is generated by transferring maleic acid isomerase and L-aspartic acid transaminase genes into the same microorganism and fermenting; regulating the isoelectric point of the ammonium L-aspartate material solution to 2.6 to 3.0 by utilizing concentrated sulfuric acid, so as to obtain a mixture of an L-aspartic acid crystal and an ammonium sulfate mother solution; furthermore, separating and drying to obtain the L-aspartic acid. By adopting the method provided by the invention, the production amount of wastewater and byproducts can be greatly reduced; the sulfuric acid and the ammonia are circularly recycled, the yield is improved, and the comprehensive cost is relatively low.

Description

technical field [0001] The invention belongs to the technical field of food industry raw material production, relates to a green production method of L-aspartic acid, and particularly relates to the improvement of refining technology. Background technique [0002] L-aspartic acid is widely used in medicine, food and chemical industry. In medicine, it is the main component of amino acid preparations, and it is the raw material for the synthesis of various drugs such as L-aspartate potassium, magnesium, calcium, asparagine, etc.; in the food industry, L-aspartic acid is a good It is a nutritional supplement added to various cool drinks; it is the main raw material for the production of sugar substitute aspartame; in chemical industry, it can be used as a raw material for the manufacture of synthetic resins, and is used in large quantities to synthesize polyaspartic acid, an environmentally friendly material; It can be used as a nutritional additive for cosmetics, etc., and ha...

Claims

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Application Information

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IPC IPC(8): C12P13/20C07C227/40C07C229/24
CPCC07C227/40C12P13/20C07C229/24
Inventor 李正华李云政万玉青孟庆强程晋超刘二丹李虎
Owner ANHUI XUELANG BIOTECHNOLOGY CO LTD
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