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A kind of method for culturing mycelium mycoplasma with high carotenoid production and its product preparation

A carotenoid and a culturing method are applied in the high-yielding carotenoid-producing mycoplasma culturing method and the product field of Mycobacterium tumefaciens, to achieve the effects of improving carotenoid content, optimizing culturing conditions and high bioavailability

Active Publication Date: 2021-01-26
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Nowadays, the research on carotenoids contained in Myrrhia has been increasing, but most of them are still in the stage of extraction and identification, and there are few reports on how to increase the content of carotenoids

Method used

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  • A kind of method for culturing mycelium mycoplasma with high carotenoid production and its product preparation
  • A kind of method for culturing mycelium mycoplasma with high carotenoid production and its product preparation
  • A kind of method for culturing mycelium mycoplasma with high carotenoid production and its product preparation

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Experimental program
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Effect test

Embodiment 1

[0087] The optimization of embodiment 1 bacterial culture condition

[0088] Inoculate the original strains preserved on a slant or a strain tube onto a PDA solid plate medium, inoculate each strain 3 times as a repetition, and culture at a constant temperature and shade at 25°C until the mycelia cover the petri dish, and then light it to make it change color , to obtain activated strains.

[0089] Take 250mL Erlenmeyer flasks, add 100mL PDA liquid medium to each bottle, seal the bottles, and sterilize at 121°C for 30min. On the activated plate covered with yellow mycelia, take 3 to 4 pieces of mycelium of the size of soybean grains and inoculate them into the prepared PDA liquid medium. After the inoculation, place it in a shaker at 25°C and 150r / min for 4-5 days, and cultivate it for 4-5 days.

[0090] Bacteria culture: each bottle weighs each ingredient according to the culture material formula, and stirs well after adding liquid. Place the bottle in a high-pressure stea...

Embodiment 2

[0112] The optimization of embodiment 2 chitosan induction conditions

[0113] When the mycelium of the mycelium is overgrown and begins to illuminate, the concentration of the inducer is scientifically and reasonably designed, and the sterilized chitosan of 2mg / mL, 1mg / mL, 0.5mg / mL and 0.05mg / mL is sprayed respectively. Spray 2mL of each concentration to determine the content of carotenoids. The results are shown in Table 2. The results show that when the chitosan concentration is 2mg / mL, it has the greatest impact on the production of carotenoids of Miracle bacteria, which is 15% higher than that of the control group, indicating that chitosan can induce Myromycetes produce carotenoids.

[0114] Table 2 Effects of Chitosan on Carotenoids Produced by Miracle

[0115]

Embodiment 3

[0116] Preparation of Example 3 Miracle Mushroom Carotenoid Powder and Determination of Nutrients

[0117] After the fermentation, the fresh Myristia culture substrate produced in Example 1 was dried in an oven at 55° C., crushed and passed through a 70-mesh sieve to obtain Myristia carotenoid powder. Then its active ingredients are measured to evaluate its nutritional value. Commercially available fruiting bodies were used as a control.

[0118] 1. Analysis and determination of polysaccharides

[0119] (1) Weigh a certain amount of sample, the ratio of solid to liquid is 1:30 (w / v), add distilled water, ultrasonically extract (400W, 5min), filter the supernatant, concentrate the filtrate to a certain volume under reduced pressure, add 4 times 95% ethanol by volume and placed in a 4°C refrigerator overnight. On the next day, centrifuge (4200r / min, 10min) to precipitate the polysaccharide, and redissolve it in water to obtain a crude polysaccharide solution of Cordyceps base...

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Abstract

The invention discloses a method for mycoplasm culture of a Mili entomogenous fungus for highly yielding carotenoids, products thereof, and preparation methods of the products. Three optimum edible media are obtained through researches and summary by creatively adopting the carotene content of the Mili entomogenous fungus as an index, and optimum culture conditions obtained through the researches and summary are as follows: the inoculation amount of a mycoplasm culture medium is 25%, the dark culture is carried out at 22-28 DEG C for 11-13 days, and illumination culture is carried out at 25 DEG C for 25-45 days. Chitosan used as an inducer for the first time further makes carotenoid yield of the Mili entomogenous fungus be highest and keep stable. The culture method has the advantages of simplicity in operation, energy saving, remarkable improvement of the yield of the carotenoids in the Mili entomogenous fungus, and wide market application prospect. The invention also provides three carotenoid-rich Mili entomogenous fungus products prepared from a mixed matrix of the media and the Mili entomogenous fungus produced through the mycoplasm culture method for improving the carotenoid content of the Mili entomogenous fungus, and preparation methods thereof.

Description

technical field [0001] The invention belongs to the field of artificial cultivation of Miracula, and more specifically relates to a method for cultivating Myrrhia with high carotenoid yield and a product thereof. Background technique [0002] In traditional Chinese medicine, some Cordyceps fungi, such as Cordyceps sinensis, Miraculina, and Cordyceps macrocystis, etc., and their complexes with the host can often be directly used as medicine. [0003] Myrrhia is a kind of entomogenous fungus, which has very similar medicinal value to Cordyceps sinensis, and contains biologically active substances such as cordycepic acid, cordycepin, cordyceps polysaccharide, steroids, mannitol and superoxide dismutase (SOD). Miracle also contains 17 kinds of amino acids, the total protein content is as high as 30%, of which 35.47% are essential amino acids. In addition, Miracle bacteria also contains VA, VD, VE, VC, VB1, VB2 and VB6 and other vitamins. In addition, Cordyceps sinensis is a tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P23/00C12R1/645
CPCC12N1/14C12P23/00
Inventor 林俊芳郭丽琼刘聪简锦辉云帆叶志伟康林芝殷林
Owner SOUTH CHINA AGRI UNIV
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