Method for simultaneously detecting ochratoxin A and zearalenone
A technology for zearalenone and ochratoxin, applied in the field of analysis and detection, can solve problems such as poor accuracy, and achieve the effects of improving accuracy and sensitivity, improving recovery rate, and high sensitivity and accuracy
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Embodiment 1
[0055] Weigh 0.01mg of ochratoxin A and 0.1mg of zearalenone respectively, mix them, dissolve them with mobile phase and dilute to 1mL, pass through a 0.45μm organic filter membrane to obtain the standard test solution;
[0056] Adopt high-performance liquid chromatograph to detect standard substance for testing solution, the HPLC spectrogram that obtains is as follows figure 1 as shown, figure 1 Among them, the peak at the retention time of 8.817min is the absorption peak of ochratoxin A, and the peak at the retention time of 10.037min is the absorption peak of zearalenone; wherein the chromatographic conditions are as follows:
[0057] Column: C 18 Column, 250mm×4.6mm, 5μm;
[0058] Mobile phase: acetonitrile: water: 2v% glacial acetic acid with a volume ratio of 49.5:49.5:1;
[0059] Flow rate: 1.0mL / min;
[0060] Detection wavelength: excitation wavelength 333nm, emission wavelength 477nm;
[0061] Injection volume: 10μL;
[0062] Column temperature: 25°C.
[0063] ...
Embodiment 2
[0082] The method for simultaneously detecting ochratoxin A and zearalenone provided in this embodiment comprises the following steps:
[0083] S1. Sample pretreatment:
[0084] Weigh 25.00g of wheat powder, add 150mL of acetonitrile and water mixed solvent with a volume ratio of 8:1, shake and extract for 40min, collect the extract, filter, absorb 10mL of the filtrate, add to 50mL of water to dilute and mix;
[0085] Take 10mL of the diluted solution and transfer it to the immunoaffinity column, adjust the pressure so that the solution passes through the immunoaffinity column at a flow rate of 1-2 drops / s, until some air enters the column, then rinse the column with water twice, wait until the water flow After drying, use 2.0mL methanol for one elution, and control its flow rate to 3 drops / 4s. After the second eluent was drained, combine the two eluents, blow dry under nitrogen at 50°C, cool the residue to room temperature, dissolve it with mobile phase and dilute to 1 mL, p...
Embodiment 3
[0095]The method for simultaneously detecting ochratoxin A and zearalenone provided in this embodiment comprises the following steps:
[0096] S1. Sample pretreatment:
[0097] Weigh 25.00 g of rice powder, add 125 mL of acetonitrile and water mixed solvent with a volume ratio of 9.5:1, shake and extract for 30 minutes, collect the extract, filter, absorb 10 mL of the filtrate, add to 30 mL of water to dilute and mix;
[0098] Take 10mL of the diluted solution and transfer it to the immunoaffinity column, adjust the pressure so that the solution passes through the immunoaffinity column at a flow rate of 1-2 drops / s, until some air enters the column, then rinse the column with water twice, wait until the water flow After drying, use 1.5mL methanol for one elution, control its flow rate to 1 drop / s, and then use 2.0mL methanol for a second elution, control its flow rate to 3 drops / 2s, wait for After the second eluent was drained, combine the two eluents, blow dry under nitrogen...
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