A strain of Mycoplasma pigeon and its application
A technology for Mycoplasma pigeonii and Mycoplasma pigeon is applied to Mycoplasma pigeonii and its application field, and can solve the problems of no vaccine for preventing Mycoplasma pigeonum, accelerated transmission speed, longer disease course, etc., and achieves good protection effect, stable biological characteristics, and strong pathogenicity. force effect
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Embodiment 1
[0019] Embodiment 1, the separation and identification of pigeon mycoplasma
[0020] 1.1 Disease data collection
[0021] The disease material came from the lungs of a racing pigeon that died of sinusitis and dyspnea in a large racing pigeon farm in Zhecheng County, Henan Province in April 2017.
[0022] 1.2 Preparation of medium
[0023] PPLO liquid medium: PPLO broth powder 10.5g, glucose 2.5g, yeast powder 2.5g, dissolved in 440mL ultrapure water, sterilized at 115°C for 15min, added MEM medium 5mL, horse serum 50mL, penicillin 80,000 units, Sterile mass fraction 10% arginine 10mL and 1% (w / v) phenol red 500μL. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.
[0024] PPLO solid medium: PPLO liquid medium added with 1.5% (w / v) agar powder. After the culture was sterilized at 115°C for 15 minutes, it was stored at 4°C for later use.
[0025] 1.3 Isolation and culture of mycoplasma
[0026] Take an appropriate amount of disea...
Embodiment 2
[0042] Embodiment 2, the preparation of vaccine, safety and efficacy test
[0043] 2.1 Preparation of vaccine
[0044] Inoculate the Mycoplasma pigeonii strain HNMco1 into the PPLO liquid medium and place it at 37°C, 5% CO 2 Cultivate in an incubator, collect the culture after 3 days, measure the concentration, and adjust the number of bacteria of Mycoplasma pigeonii in the culture to 2×10 7 CFU / mL, add 2mL formaldehyde solution (mass fraction: 37%) for every 1000mL culture, inactivate at 37°C for 12h to prepare the vaccine stock solution; then add aluminum hydroxide adjuvant (Sigma, F5881) equal to the volume of the vaccine stock solution , mixed to make the inactivated vaccine of Mycoplasma pigeonii, the number of bacteria of Mycoplasma pigeonii in the vaccine is about 1×10 7 CFU / mL.
[0045] 2.2 Vaccine safety test:
[0046] Select 20 healthy pigeons aged 45 days. Divided into 4 groups, 5 in each group. Vaccine group 3 groups, each group was subcutaneously injected wi...
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