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A preparation method of biomimetic collagen-based material for actively guiding tissue regeneration and repair

A technology of tissue regeneration and active guidance, which is applied in the field of preparation of biomimetic collagen-based materials, can solve single and other problems, and achieve the effect of accelerating tissue regeneration and wound repair

Active Publication Date: 2020-09-15
无锡贝迪生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to overcome the defect that the existing single collagen material can only passively promote wound repair, and provide a preparation method of a bionic collagen-based material that actively guides tissue regeneration and repair

Method used

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  • A preparation method of biomimetic collagen-based material for actively guiding tissue regeneration and repair
  • A preparation method of biomimetic collagen-based material for actively guiding tissue regeneration and repair
  • A preparation method of biomimetic collagen-based material for actively guiding tissue regeneration and repair

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Effect test

Embodiment 1

[0029] A method for preparing a biomimetic collagen-based material that actively guides tissue regeneration and repair, comprising the following steps:

[0030] 1), preparation of low immunogenicity, high purity bioactive collagen with a mass concentration of 0.5%;

[0031] 2) Add acetic anhydride to the collagen, stir at 4°C, pH 7, acylation reaction for 6 hours, the amount of acetic anhydride added is 5g / 100g collagen;

[0032] 3), add hyaluronic acid with a molecular weight of 180kDa to 2), mix well, centrifuge to remove air bubbles, then pour into a mold, and freeze-dry to obtain a collagen-glycosaminoglycan composite sponge with a porous network structure; hyaluronic acid The mass ratio of acid to collagen is 1:8;

[0033] 4) Place the collagen-glycosaminoglycan composite sponge in the EDC / NHS cross-linking system for cross-linking, adjust the degree of cross-linking with L-polylysine, shake the cross-linking reaction at room temperature for 6 hours, and then wash with d...

Embodiment 2

[0036] A method for preparing a biomimetic collagen-based material that actively guides tissue regeneration and repair, comprising the following steps:

[0037] 1), preparation of low immunogenicity, high purity bioactive collagen with a mass concentration of 1.5%;

[0038] 2) Add succinic anhydride to the collagen, stir and react at 15°C and pH 10 for 1 hour to acylate the collagen; the amount of succinic anhydride added is 20g / 100g collagen;

[0039] 3), add chondroitin sulfate with a molecular weight of 40kDa to 2), mix well, centrifuge, remove air bubbles, then pour into the mold, and freeze-dry to obtain a collagen-glycosaminoglycan composite sponge with a porous network structure; sulfuric acid The mass ratio of chondroitin to collagen is 1:20;

[0040] 4) Put the collagen-glycosaminoglycan composite sponge in the EDC / NHS cross-linking system for cross-linking, adjust the degree of cross-linking with L-polylysine, shake and react at room temperature for 12 hours, then w...

Embodiment 3

[0043] A method for preparing a biomimetic collagen-based material that actively guides tissue regeneration and repair, comprising the following steps:

[0044] 1), preparation of low immunogenicity, high-purity bioactive collagen with a mass concentration of 1.2%;

[0045] 2) Add phthalic anhydride to the collagen, and stir and react for 3 hours at 10°C with a pH of 9 to acylate the collagen; the amount of acid anhydride added is 10g / 100g collagen;

[0046] 3), add chitosan to 2), after mixing evenly, centrifuge to remove air bubbles, then pour into the mold, and freeze-dry to obtain a collagen-glycosaminoglycan composite sponge with a porous network structure; glycosaminoglycan and The mass ratio of collagen is 1:14;

[0047] 4) Place the collagen-glycosaminoglycan composite sponge in the EDC / NHS cross-linking system for cross-linking, adjust the degree of cross-linking with L-polylysine, shake and react at room temperature for 10 hours, then wash with distilled water to re...

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Abstract

The invention discloses a preparation method of a bionic collagen based material actively guiding tissue regeneration and repair. The preparation method includes the following steps that 1, bioactive collagen with low immunogenicity and high purity is prepared; 2, anhydride is added to enable the collagen to be subjected to acylation; 3, glycosaminoglycan is added and reacted to obtain a collagen-glycosaminoglycan composite sponge of a porous network structure; 4, the sponge is placed in an EDC / NHS crosslinking system to be subjected to crosslinking, and a crosslinked collagen-glycosaminoglycan composite sponge is obtained; and 5, the crosslinked collagen-glycosaminolgycan composite sponge is soaked in a growth factor solution and reacted to obtain the bionic collagen based material actively guiding tissue regeneration and repair. The preparation method solves the problem that the collagen-glycosaminoglycan is prone to precipitating in the compositing process; regulation of a microscopic network structure (pore size and porosity) and the mechanical strength of the material is achieved, and finally matching of the angiogenesis speed and the healing period of a wound surface and the material properties is achieved, and growth factors actively guide repair of the wound and tissue regeneration through sustained or controlled release.

Description

technical field [0001] The invention relates to a wound repair material, in particular to a preparation method of a bionic collagen-based material that actively guides tissue regeneration and repair. Background technique [0002] Wound repair materials are a class of clinical medical materials used for postoperative wound care, burns, external trauma and long-term non-healing ulcer wound repair, mainly including traditional wound care products (such as cotton gauze bandages, impregnated cotton gauze, cotton pads, etc.) and high-end Wound repair materials. Compared with traditional wound care products, high-end wound repair materials are developed based on the theory of moist wound healing, which can improve the speed and quality of wound healing, solve the problems of chronic and complex wounds that are difficult to heal, reduce the pain of patients, and have lower replacement frequency, which is more in line with modern times. Medical development needs and mainstream techn...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L31/04A61L31/16A61L31/14A61L27/26A61L27/54A61L27/56A61L27/58A61L27/60
CPCA61L27/26A61L27/54A61L27/56A61L27/58A61L27/60A61L31/041A61L31/146A61L31/148A61L31/16A61L2300/236A61L2300/252A61L2300/412A61L2300/414A61L2300/602C08L5/08C08L5/10C08L89/00
Inventor 陆金婷程咏梅任伟业
Owner 无锡贝迪生物工程股份有限公司
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