Separation and enrichment method for camptothecin-containing plant hydroponic root system allelochemical

A technology for the separation and enrichment of allelochemical substances, which is applied to the purification/separation of amino compounds, the separation/purification of hydroxyl compounds, the separation/purification of carbonyl compounds, etc., and can solve the problems of growth inhibition, medicinal plant continuous cropping obstacles, and self-poisoning and other issues to achieve the effect of promoting vigorous growth

Active Publication Date: 2017-12-01
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, the growth inhibitory effect of some allelochemicals on plants has a certain negative impact on production. For example, allelochemicals in root exudates cause continuous cropping obstacles in medicinal plants, and phenolic compounds in allelochemicals can affect rice, etc. Metabolic pathways in plants that produce autotoxic phenomena that stunt growth

Method used

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  • Separation and enrichment method for camptothecin-containing plant hydroponic root system allelochemical
  • Separation and enrichment method for camptothecin-containing plant hydroponic root system allelochemical
  • Separation and enrichment method for camptothecin-containing plant hydroponic root system allelochemical

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Embodiment 1

[0048] The method of the present invention will be further described below in conjunction with specific embodiments and accompanying drawings. The following examples are for illustrative purposes only, and should not be construed as limiting the present invention. Unless otherwise specified, the biological materials and reagent raw materials used in the following examples are commercially available or commercially obtained biological materials and reagent raw materials. Unless otherwise specified, the methods and equipment used in the following examples are routinely used in the art methods and equipment. Example 1 Enrichment and Separation of Allelochemicals in Roots of Snakeroot Hydroponic Culture

[0049] 1. Construction of snakeroot hydroponic system

[0050] 1.1 Snakeroot hydroponics and related operations

[0051] After the Guangzhou snakeroot is collected, select a well-growing plant and gently wash it with tap water to remove the soil at the root. Try not to damage ...

Embodiment 2

[0073] Example 2 Enrichment analysis of allelochemicals in root exudates of Campylodon chinensis

[0074] 1. Construction of camphor tree hydroponic system

[0075] 1.1 Operations related to camptophylla hydroponics

[0076] Exploration on the construction of a hydroponic system for camphor tree seedlings: select seedlings with good growth of about 50 cm, and do not damage the roots when digging. One group washed the root soil and placed it outdoors for cultivation.

[0077] 1.2 Enrichment of root exudate

[0078] After 60 days of hydroponic cultivation, the hydroponic fluid was collected and filtered to remove solid impurities. Using silica gel as a filler, SPE solid phase extraction is used to enrich the hydroponic liquid in batches to adsorption equilibrium, and the silica gel is replaced. After enrichment, the silica gel containing root exudates was collected, dried at 45°C, and stored at 4°C for later use.

[0079] 1.3 Separation and detection process of concentrated...

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Abstract

The invention discloses a separation, enrichment and detection method for a camptothecin-containing plant hydroponic root system allelochemical. The method comprises the following steps: firstly taking silica gel as a filler, filling a solid-phase extraction column, extracting and adsorbing root system metabolites for plant hydroponic solution, then taking out the filled silica gel, loading to an upper layer of a silica gel chromatography column, carrying out column chromatography isolation, then eluting in different combination ratios by taking petroleum ether, ethyl acetate, ethanol and chloroform as eluents, collecting eluate according to the composition of the eluate, carrying out rotary evaporation and concentration, and analyzing a separated component by utilizing a GC-MS technology. The method disclosed by the invention has the advantages that separation, enrichment and detection on a large number of allelochemicals in two kinds of plant hydroponic root secretions are realized; 25 allelochemicals are separated from camptotheca acuminate hydroponic root secretions and ophiorrhiza japonica hydroponic root secretions in total; and the method disclosed by the invention has very important technical guiding effect on ascertaining of more allelochemicals in a hydroponic system, substances with autotoxicity in the allelochemicals can be eliminated in time based on the method disclosed by the invention, plant growth cannot be inhibited, and vigorous growth of plants is promoted, so that the method disclosed by the invention has important significance on optimization of the plant hydroponic system.

Description

technical field [0001] The invention relates to the technical field of separation and preparation of compounds, and more specifically, relates to a technology for the separation and identification of allelochemicals in roots of hydroponic plants containing camptothecin. Background technique [0002] Camptothecin (CPT) is an important anti-cancer active alkaloid found from camptotheca japonica, and a certain amount was also found in snakeroot plants later. It is the only natural active ingredient discovered so far that exerts cytotoxicity by inhibiting topoisomerase. The market demand for camptothecin anticancer drugs is very large, but large-scale chemical total synthesis and in vitro biosynthesis are more difficult. Extracting camptothecin from a large number of plant resources such as camptothecin is currently an important way to obtain camptothecin . However, long-term blind collection will lead to the destruction of the ecological environment and the waste of plant res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07B63/00C07C37/82C07C17/389C07C17/38C07C19/055C07C209/86C07C211/47C07C39/06C07C69/80C07C13/567C07C7/12C07C67/56C07C69/24C07C39/08C07C39/16C07C29/76C07C31/20C07C45/79C07C49/717C07C211/46C07F11/00
CPCC07B63/00C07C7/005C07C7/12C07C17/38C07C17/389C07C29/76C07C37/004C07C45/79C07C67/56C07C209/86C07D249/18C07F11/00C07C19/055C07C211/47C07C39/06C07C69/80C07C13/567C07C69/24C07C39/08C07C39/16C07C31/20C07C49/717C07C211/46
Inventor 程杏安刘展眉蒋旭红林贤伟
Owner ZHONGKAI UNIV OF AGRI & ENG
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