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Method for detecting MG based on Au-Ag NPs composite material

A malachite green and composite material technology, which is applied in the fields of nanotechnology and food safety detection, can solve problems such as interference, and achieve the effects of low detection limit, strong specificity, and simple and fast operation.

Inactive Publication Date: 2017-11-24
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the need to prepare highly active enhanced substrates for SERS detection and the interference of non-target compounds in the matrix to be tested, there are still certain limitations in the application of SERS in the quantitative detection of trace residues in complex matrices.

Method used

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  • Method for detecting MG based on Au-Ag NPs composite material
  • Method for detecting MG based on Au-Ag NPs composite material
  • Method for detecting MG based on Au-Ag NPs composite material

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The preparation of HQ-assisted dendritic Au-Ag NPs composites, the specific steps are as follows:

[0039] (1) Preparation of Ag seeds nanoparticle sol: adopt the classic sodium citrate reduction method, that is: take 1.0 mM AgNO 3 Put 25 ml of the solution in a 100 ml three-necked flask, stir magnetically, heat to reflux until boiling, then quickly add 0.5 ml of 1 wt% sodium citrate solution, and continue to boil for 1 h to obtain a yellow-green solution;

[0040] (2) Take HAuCl at a concentration of 0.5 mM 4 Solution 10ml, then add 0.6 ml of the Ag seeds solution prepared in the previous step and mix magnetically for 2 min, then add 1.0 ml of 30 mM HQ solution and continue magnetically stirring for 30 min to obtain dendritic Au-AgNPs composites.

[0041] The detection of MG standard solution, the specific steps are as follows:

[0042] (1) First prepare MG standard solutions with gradient concentrations (0.00027 μM, 0.00068 μM, 0.0014 μM, 0.0027 μM, 0.0068 μM, 0.014...

Embodiment 2

[0046] The detection of MG content in fish, the specific steps are as follows:

[0047] (1) Actual sample processing: Take about 1500 g of fresh carp purchased from Yonghui Supermarket, slaughter it and remove the skin and bones, use a meat grinder to grind the fish meat, and store it in the freezer of the refrigerator for later use;

[0048] (2) MG standard solutions of different concentrations were added to equal parts of fish to make (0 ng / g, 0.05 ng / g, 0.10 ng / g, 0.20 ng / g, 0.50 ng / g, 1.0 ng / g, 5.0 ng / g) MG fish samples with gradient concentration; take 5.00±0.01 g fish samples with different MG contents, add 1.0 ml 9.5 g / l hydroxylamine hydrochloride solution, let stand for 15 min, take 20 ml acetonitrile, 2 g anhydrous Add magnesium sulfate, vortex for 1 min, add 1.0 ml of 0.001 M 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ) acetonitrile solution for 10 min, then add 8 g Vortex the alumina for 30 s, sonicate for 10 min, centrifuge at 13000 rpm for 15 min, transfer th...

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Abstract

The invention provides a method for detecting MG based on an Au-Ag NPs composite material, and belongs to the field of nano-technology and food safety detection. Preparation of a dendritic Au-Ag NPs active substrate relates to preparation of Ag seeds nano-sol and Galvani replacement reaction with HAuCl4, and then, hydroquinone (HQ) serves a reducing agent to promote the formation of a dendritic structure. During a synthesizing process, the adding amounts of the Ag seeds, the HAuCl4 and the HQ are selected, the nano-particles with optimal size and morphology are obtained to serve as an active substrate, and the MG is detected through a surface enhanced raman scattering (SERS) technology. The active substrate obtained by the method has the advantages of high SERS enhancement property, high reproducibility and the like. An MG detecting result shows that the method provided by the invention can detect the content of the MG in fish sensitively, quickly and effectively, and the lowest detecting content is 0.05 ng / g.

Description

technical field [0001] The invention relates to a method for detecting malachite green based on an Au-Ag NPs composite material, belonging to the fields of nanotechnology and food safety detection. Background technique [0002] Malachite green (MG) is the most representative substance among triphenylmethane dyes, and has a maximum absorption at 618nm. Because of its strong antibacterial, bactericidal, and parasite-inhibiting effects, it is widely used in aquaculture (see literature: Z. Y. Guo, P. P. Gai, T. T. Hao, J. Duan, S. Wang, J. Agric. Food Chem ., 2011, 59(10), 5257; D. Hurtaud-Pessel, P. Couëdor, E. Verdon, Journal of Chromatography A, 2011, 1218, 1632; R. A. Schnick, Prog. Fish Cult., 1988,50, 190; Z. Z. Lin, H.Y. Zhang, A. H. Peng, Y. D. Lin, L. Li, Z. Y. Huang, Food Chemistry, 2016, 200, 32). However, studies have shown that malachite green and its metabolites have side effects such as high toxicity, high residue, carcinogenicity, teratogenicity, and mutagenesi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 许雪琴王敬
Owner FUZHOU UNIV
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