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A preparation method of antibacterial periodontal tissue regeneration film

A periodontal tissue regeneration and nanofiber technology is used in the preparation of regenerated membranes and the preparation of antibacterial periodontal tissue regeneration membranes, to achieve the effect of avoiding immunogenicity and slow release difficulties

Active Publication Date: 2018-05-25
郭婷
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art and provide a preparation method of antibacterial periodontal tissue regeneration membrane, which simulates the extracellular matrix structure of periodontal tissue and provides cells with growth space; Under the condition of any growth factor, the mineralization and differentiation of stem cells can be induced, thereby avoiding the problems of immunogenicity and slow-release difficulties caused by the addition of induction factors to conventional tissue engineering techniques. Fluorapatite releases fluoride ions by slow release, which can be antibacterial

Method used

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  • A preparation method of antibacterial periodontal tissue regeneration film

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Effect test

Embodiment 1

[0028] The preparation method of a kind of antibacterial periodontal tissue regeneration film provided in this embodiment, the electrospinning method is used to prepare polycaprolactone nanofiber scaffold, utilize HEDTA, Ca(NO 3 ) 2 , KF, KOH and KH 2 PO 4 Chemical reaction to crystallize fluoroapatite on the surface of the nanofiber scaffold, the specific operation is:

[0029] (1) Prepare liquid A: choose 0.5M HEDTA, 0.5M Ca(NO 3 ) 2 and 0.65M KOH for stirring and fully mixing for later use;

[0030] (2) Preparation of liquid B: use 0.3M KH 2 PO 4 and 0.1M KF for stirring and mixing fully for later use, then mix liquid A and liquid B in step (1) for full reaction and set aside;

[0031] (3) Immerse the PCL nanofiber scaffold prepared by the electrospinning method into the mixed solution prepared in step (2), and put it in a water bath with a water bath temperature of 37°C, shake for 10 minutes every 3 hours, and harvest the scaffold after 24 hours , and then the scaf...

Embodiment 2

[0033] The preparation method of a kind of antibacterial periodontal tissue regeneration film provided in this embodiment, the electrospinning method is used to prepare polycaprolactone nanofiber scaffold, utilize HEDTA, Ca(NO 3 ) 2 , KF, KOH and KH 2 PO 4 Chemical reaction to crystallize fluoroapatite on the surface of the nanofiber scaffold, the specific operation is:

[0034]1) Prepare liquid A: choose 0.7M HEDTA, 0.3M Ca(NO 3 ) 2 and 0.7M KOH for stirring and fully mixing for later use;

[0035] (2) Preparation of liquid B: use 0.5M KH 2 PO 4 and 0.1M KF for stirring and mixing fully for later use, then mix liquid A and liquid B in step (1) for full reaction and set aside;

[0036] (3) Immerse the PCL nanofiber scaffold prepared by the electrospinning method into the mixed solution prepared in step (2), and put it in a water bath, the temperature of the water bath is 40°C, shake for 20 minutes every 5 hours, and harvest the scaffold after 20 hours , and then the sc...

Embodiment 3

[0038] In the preparation method of the aforementioned antibacterial periodontal tissue regeneration film, the electrospinning method is used to prepare polycaprolactone nanofiber scaffolds, and HEDTA, Ca(NO 3 ) 2 , KF, KOH and KH 2 PO 4 Chemical reaction to crystallize fluoroapatite on the surface of the nanofiber scaffold, the specific operation is:

[0039] 1) Prepare liquid A: choose 0.6M HEDTA, 0.4M Ca(NO 3 ) 2 and 0.68M KOH for stirring and fully mixing for later use;

[0040] (2) Preparation of liquid B: use 0.4M KH 2 PO 4 and 0.2M KF for stirring and mixing fully for later use, then mix liquid A and liquid B in step (1) for full reaction and set aside;

[0041] (3) Immerse the PCL nanofiber scaffold prepared by the electrospinning method into the mixed solution prepared in step (2), and put it in a water bath with the temperature of the water bath at 39°C, shake for 15 minutes every 4 hours, and harvest the scaffold after 22 hours , and then the scaffold was ri...

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Abstract

The invention discloses a preparation method of an antibacterial periodontium reproductive membrane. A polycaprolactone nano fiber stent is prepared by applying an electrospinning method. By use of chemical reaction of HEDTA (Hydroxyethyl Ethylenediamine Triacetic Acid), KF (potassium fluoride), KOH (potassium hydroxide) and KH2PO4 (monopotassium phosphate), fluorapatite is crystallized on the surface of the nano fiber stent. The preparation method disclosed by the invention is simple and feasible; the prepared reproductive membrane can be used for simulating a periodontal tissue cell epimatrix structure to provide a growth space for cells; under a condition of not adding any growth factors, stem cells are induced to be mineralized and differentiated, so that the problems of immunogenicity and slow release difficulty which are caused by the fact that induction factors are required to be added in conventional tissue engineering are solved; the fluorapatite slowly releases fluorinions which can resist bacteria.

Description

technical field [0001] The invention relates to a preparation method of a regeneration film, in particular to a preparation method of an antibacterial periodontal tissue regeneration film, which can be used for repairing defective tissues and belongs to the field of biomedical materials. Background technique [0002] Periodontal disease is one of the most common and frequent diseases in humans, the most common of which is periodontitis. The significant pathological changes of periodontal disease are the destruction of periodontal supporting tissue, alveolar bone resorption, and the bone attached to the bone. The destruction of connective tissue is accompanied by different degrees of cementum resorption, and if it is not treated effectively, it will lead to loosening and loss of teeth; generally speaking, the key to obtaining periodontal tissue regeneration in clinical practice is the use of shielding membrane, which shields The role of the membrane is to organize the fixed v...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/18A61L27/12A61L27/54
CPCA61L27/12A61L27/18A61L27/54A61L2300/106A61L2300/112A61L2300/404A61L2300/412A61L2300/604A61L2400/12A61L2430/12C08L67/04
Inventor 郭婷曹罡毛钊
Owner 郭婷
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