Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of enrichment and solid-phase derivatization pretreatment method of phosphate sugars

A technology for phosphate sugars and sugars, which is applied in the field of phosphate sugar enrichment and solid-phase derivatization pretreatment to improve the detection sensitivity of mass spectrometry, lower detection limits, and remove matrix interference.

Active Publication Date: 2019-12-06
DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports on the pretreatment method of enrichment and solid-phase derivatization of phosphate sugars based on immobilized metal ion affinity chromatography materials

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of enrichment and solid-phase derivatization pretreatment method of phosphate sugars
  • A kind of enrichment and solid-phase derivatization pretreatment method of phosphate sugars
  • A kind of enrichment and solid-phase derivatization pretreatment method of phosphate sugars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1. immobilized metal affinity chromatography material SiO 2 @PD-Ti 4 The synthesis of + and its enrichment and derivation process for phosphate sugars.

[0017] The first step to synthesize immobilized metal affinity chromatography material SiO 2 @PD-Ti 4+ : 200mg SiO2 2 (particle size 3μm) was dispersed in 40mL dopamine aqueous solution with a concentration of 2mg / mL, and 40mL Tris buffer solution (pH 8.5) was quickly added under magnetic stirring, and reacted for 10 hours at a water bath temperature of 40°C under magnetic stirring conditions, and washed with water to obtain SiO 2 @PD microspheres. Then SiO 2 @PD dispersed in 80mL of Ti(SO 4 ) 2 In the solution, the reaction was continued for 3 hours at a water bath temperature of 40°C under magnetic stirring conditions, and SiO was obtained after washing with water. 2 @PD-Ti 4+ Microspheres. Synthesis schematic see figure 1 .

[0018] The second enrichment derivation process: take 6-phosphate g...

Embodiment 2

[0019] Example 2. Selective Enrichment of Phosphosaccharides in Derived Cells and LC-MS Analysis

[0020](1) Cell pretreatment: 7721 liver cancer cells were taken out from the incubator, quenched instantly with liquid nitrogen, and washed 3 times with PBS buffer. Add 1mL of methanol, scrape the cells and transfer them to the EP tube, vortex for 30s, then add 400μL of ultrapure water, vortex for 30s, finally add 1mL of chloroform, and vortex for 30s. After standing still for 1 min, centrifuge at 15000 rpm / min for 10 min. Take 900 μL of supernatant and freeze-dry. Add 4mL sample solution (by ACN / H 2 O (65 / 35, v / v) solution was prepared by adding a final concentration of 2% (v / v) formic acid (FA) and a final mass fraction of 0.4% glutamic acid) for reconstitution.

[0021] (2) Material pretreatment process: take 10mg SiO 2 @PD-Ti 4+ Material, with sample solution (by ACN / H 2 O (65 / 35, v / v) solution was prepared by adding a final concentration of 2% (v / v) formic acid (FA) an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
concentrationaaaaaaaaaa
thicknessaaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of inorganic materials and analysis, in particular to a metal ion affinity chromatography material (SiO 2 @PD‑Ti 4+ ) pretreatment method for enrichment and solid-phase derivatization of phosphate carbohydrates and its application. First, phosphate sugars were enriched on SiO by metal chelation 2 @PD‑Ti 4+ On the microspheres, the enriched phosphate sugars are then subjected to solid-phase derivatization. The method can not only improve the retention and resolution of sugar phosphate substances on reversed-phase liquid chromatography, but also improve the detection sensitivity of mass spectrometry for sugar phosphate substances with low abundance. The pretreatment method of the present invention has the advantages of simple pretreatment process, rapidity, good repeatability, low detection limit and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic materials and analysis, and is a kind of material based on metal ion affinity chromatography (SiO 2 @PD-Ti 4+ ) pretreatment method for enrichment and solid-phase derivatization of phosphate sugars and its application. Background technique [0002] Phosphoric sugars such as glucose-6-phosphate, ribose-5-phosphate, erythrose-4-phosphate and glyceraldehyde-3-phosphate play important roles in many life processes. They are important intermediate metabolites in central carbon metabolism, such as glycolysis, gluconeogenesis, pentose phosphate pathway and tricarboxylic acid cycle metabolic pathway, and can participate in the regulation of tumor energy metabolism. At present, liquid chromatography-mass spectrometry (LC-MS) technology has been widely used in the detection of phosphate sugars due to its advantages of high sensitivity and high resolution. However, due to the strong polarity of phosphate...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/06G01N30/89G01N30/02G01N27/62
CPCG01N27/626G01N30/02G01N30/06G01N30/89G01N2030/027G01N2030/067
Inventor 许国旺秦倩石先哲王博弘
Owner DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com