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Preparation method of gelatin sponge scaffold

A technology of gelatin sponge and gelatin, which is applied in the fields of pharmaceutical formulation, application, and medical science, and can solve the problems of host damage, low degree of cross-linking, and poor stability, and achieve excellent comprehensive performance, good porosity, and good biocompatibility Effect

Inactive Publication Date: 2017-10-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, chemical cross-linking agents are more or less cytotoxic. As the cross-linked material degrades in vivo, the cross-linking agent participating or remaining in the material will be released, causing harm to the host.
Although physical cross-linking is non-toxic and harmless, it has the disadvantages of low cross-linking degree and poor stability. Simple physical cross-linking cannot achieve rapid cross-linking.

Method used

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  • Preparation method of gelatin sponge scaffold
  • Preparation method of gelatin sponge scaffold
  • Preparation method of gelatin sponge scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 The preparation method of sponge support of the present invention

[0031] 1. Preparation method

[0032] (1) Hydrogel preparation: Gelatin (Type A, 300 Bloom; Sigma, USA) was quantitatively weighed and dissolved in deionized water at 50°C to obtain a 4% (w / v) solution, which was sterilized by filtration with a 0.22 μm needle filter. mTG (Bomei Bio, China, enzyme activity > 100 U / g) was dissolved in phosphate buffered saline (PBS) to obtain a 10% (w / v) solution, and sterilized by filtration. Prepare a mixed solution according to the ratio of adding 40 μm of TG solution per ml of gelatin solution, and place it in a constant temperature incubator at 37° C. until gelatinized.

[0033] (2) Preparation of the sponge scaffold: the hydrogel was freeze-dried, specifically at -10°C to -20°C for 8 hours, and then dried at 15 to 25°C for 48 hours to obtain the sponge scaffold.

Embodiment 2

[0034] Embodiment 2 The preparation method of sponge support of the present invention

[0035] 1. Preparation method

[0036] (1) Hydrogel preparation: Gelatin (Type A, 300 Bloom; Sigma, USA) was quantitatively weighed and dissolved in deionized water at 50°C to obtain a 2% (w / v) solution, which was sterilized by filtration with a 0.22 μm needle filter. mTG (Bomei Bio, China, enzyme activity > 100 U / g) was dissolved in phosphate buffered saline (PBS) to obtain a 5% (w / v) solution, and sterilized by filtration. Prepare a mixed solution according to the ratio of adding 40 μm of TG solution per ml of gelatin solution, and place it in a constant temperature incubator at 37° C. until gelatinized.

[0037] (2) Preparation of the sponge scaffold: the hydrogel was freeze-dried, specifically at -10°C to -20°C for 8 hours, and then dried at 15 to 25°C for 48 hours to obtain the sponge scaffold.

Embodiment 3

[0038] Embodiment 3 The preparation method of sponge support of the present invention

[0039] 1. Preparation method

[0040] (1) Hydrogel preparation: Gelatin (type A, 300 Bloom; Sigma, USA) was quantitatively weighed and dissolved in deionized water at 50°C to obtain a 10% (w / v) solution, which was sterilized by filtration with a 0.22 μm needle filter. mTG (Bomei Bio, China, enzyme activity > 100 U / g) was dissolved in phosphate buffered saline (PBS) to obtain a 15% (w / v) solution, and sterilized by filtration. Prepare a mixed solution according to the ratio of adding 66.7 μl of mTG solution per ml of gelatin solution, and place it in a constant temperature incubator at 37° C. until gelatinized.

[0041] (2) Preparation of the sponge scaffold: the hydrogel was freeze-dried, specifically at -10°C to -20°C for 8 hours, and then dried at 15 to 25°C for 48 hours to obtain the sponge scaffold.

[0042] The beneficial effect of the present invention is illustrated below in the mo...

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Abstract

The invention discloses a gelatin sponge scaffold. A preparation method of the gelatin sponge scaffold comprises the following steps: (1) preparing gelatin solution; (2) preparing transglutaminase solution; and (3) mixing the gelatin solution prepared in the step (1) and the transglutaminase solution prepared in the step (2), carrying out incubating, thus forming hydrogel, carrying out refrigerating, and carrying out freeze drying, thus preparing the sponge scaffold. The sponge scaffold is free of cytotoxicity, and has excellent physical property, excellent comprehensive performance, good porosity, good compression modulus, and good degradation resistance; in addition, the biocompatibility is good, so that cells can well grow in the scaffold; the scaffold is implanted into the body, a formed material wrapping layer is thin, blood capillaries are formed on the outer wrapping layer, and thus the growth of the regenerated tissue is facilitated; the gelatin sponge scaffold can be taken as the materials such as the tissue engineering scaffold, clinical hemostatic sponge and medicine controlled-release carriers to be used for the cell and tissue engineering applications, and has the broad prospect.

Description

technical field [0001] The invention relates to a preparation method of a gelatin sponge support. Background technique [0002] Gelatin is a partial hydrolysis product of collagen. Due to its non-toxic, non-carcinogenic, and good biocompatibility and biodegradability, gelatin is widely used in medicine and medical fields, such as wound dressing materials, tissue engineering scaffolds and drug carriers. . Tissue engineering scaffold materials refer to materials that can be combined with living cells of tissues and implanted into different tissues of the organism, and replace the functions of the tissues according to specific conditions. Repair scaffolds suitable for cell and tissue engineering applications should have the following characteristics: (1) non-cytotoxicity; (2) good bio-histocompatibility; (3) biodegradable without any residue; (4) rich sources of raw materials , low cost; (5) good mechanical properties; (6) good three-dimensional structure, uniform voids, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/56A61L27/50A61L27/22A61L24/00A61L24/10A61K47/42
CPCA61K9/0002A61K47/42A61L24/001A61L24/0036A61L24/104A61L27/222A61L27/50A61L27/56A61L2400/04
Inventor 杨刚龙海燕肖正华马坤龙任小梅
Owner SICHUAN UNIV
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