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Gelatin hydrogel and preparation method and application thereof

A hydrogel and gelatin technology, applied in the field of gelatin hydrogel and its preparation, can solve the problems of poor mechanical properties of pure gelatin hydrogel, cannot maintain a gel state, and limit the scope of application, and achieves overcoming poor mechanical strength, The effect of increasing the cross-linking point and realizing the degree of cross-linking

Inactive Publication Date: 2017-10-10
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two methods have their own disadvantages: firstly, pure gelatin hydrogel has poor mechanical properties, is easily soluble in water at body temperature and cannot maintain its gel state, which limits its application range; secondly, chemically crosslinked hydrogel Commonly used crosslinking agents include formaldehyde, glutaraldehyde, EDC and genipin. Aldehydes are highly toxic, and the high prices of EDC and genipin limit their application.

Method used

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  • Gelatin hydrogel and preparation method and application thereof
  • Gelatin hydrogel and preparation method and application thereof
  • Gelatin hydrogel and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] A kind of preparation of gelatin hydrogel, comprises the steps:

[0047] (1) Weigh 10 g of gelatin and add it to 100 mL of deionized water, swell at room temperature for 1 h, and stir in a constant temperature water bath at 60°C until completely dissolved. Add 16mL of methacrylic anhydride dropwise to the gelatin solution at a rate of 1mL / min, shake and react in a constant temperature shaker at 50°C for 4h, then add 400mL of deionized water at 50°C to dilute the reaction solution, and fill the diluted solution with Put it into a 8-14kDa dialysis bag, dialyze in deionized water at 50°C for 1 week, replace the deionized water every 4 hours, centrifuge the dialysate at 3000rpm for 10min after the end, take the supernatant, and put it in a -80°C refrigerator overnight, and freeze-dried for 3 days to obtain methacrylamide-based gelatin with a modification degree of 88%.

[0048] (2) Prepare 50 mL of an aqueous solution of Irgacure 2959 with a mass concentration of 0.25 wt%,...

Embodiment 2

[0050] A kind of preparation of gelatin hydrogel, comprises the steps:

[0051] (1) Weigh 10 g of gelatin and add it to 100 mL of deionized water, swell at room temperature for 1 h, and stir in a constant temperature water bath at 60°C until completely dissolved. Add 16mL of methacrylic anhydride dropwise to the gelatin solution at a rate of 1mL / min, shake and react in a constant temperature shaker at 50°C for 4h, then add 400mL of deionized water at 50°C to dilute the reaction solution, and fill the diluted solution with Put it into a 8-14kDa dialysis bag, dialyze in deionized water at 50°C for 1 week, replace the deionized water every 4 hours, centrifuge the dialysate at 3000rpm for 10min after the end, take the supernatant, and put it in a -80°C refrigerator overnight, and freeze-dried for 3 days to obtain methacrylamide-based gelatin with a modification degree of 88%.

[0052] (2) Prepare 50 mL of an aqueous solution of Irgacure 2959 with a mass concentration of 0.5 wt%, ...

Embodiment 3

[0062] A kind of preparation of gelatin hydrogel, comprises the steps:

[0063] (1) Weigh 1000g of gelatin and add it to 10L of deionized water, swell at room temperature for 1 hour, and stir in a constant temperature water bath at 60°C until completely dissolved. Add 800mL of methacrylic anhydride dropwise to the gelatin solution at a rate of 50mL / min, stir and react in a reactor at 50°C for 4h, then add 40L of deionized water at 50°C to dilute the reaction solution, and carry out ultra- Filtrate for 8 hours, use a filter membrane of 8 kDa, place the filtrate in a -80°C refrigerator overnight, and freeze-dry for 3 days to obtain methacrylamide-based gelatin with a modification degree of 75%.

[0064] (2) Configure 1 L of Irgacure 2959 aqueous solution with a mass concentration of 0.5 wt%, place it in a constant temperature water bath at 30°C and stir to dissolve, take 200 g of lyophilized methacrylamide-based gelatin and stir to dissolve, then add polyethylene glycol diacryla...

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Abstract

The invention discloses gelatin hydrogel, which is of a water molecule filled porous three-network structure, wherein the first network is a cross-linked network between the modified gelatin; the second network is a cross-linked network between polyethylene glycol diacrylate; the third network is a cross-linked network between the modified gelatin and the polyethylene glycol diacrylate. The invention also discloses a preparation method of the gelatin hydrogel. By using an ultraviolet light catalytic crosslinking technology, the modified gelatin is cross-linked with the polyethylene glycol diacrylate; the gelatin hydrogel is obtained through preparation. The gelatin hydrogel provided by the invention has the advantages that the elasticity modulus and the degrading performance of the support frame can be regulated and controlled through changing the modification degree of the modified gelatin and the addition quantity of the polyethylene glycol diacrylate; the structure regulation can be better performed according to the requirements of different tissue engineering support frames; the proper space and the intensity can be provided for the growth of the cells; the ordered space structure is formed.

Description

technical field [0001] The invention relates to the technical field of tissue engineering. More specifically, it relates to a gelatin hydrogel and its preparation method and application. Background technique [0002] Gelatin is a natural macromolecule obtained by hydrolysis of collagen. Its protein structure determines its good biocompatibility and biodegradability. Compared with collagen, it has no immunogenicity, strong processability, and retains collagen Contains cell adhesion peptides and protease degradation sites. Therefore, gelatin hydrogels and gelatin-based composite hydrogels are widely used in tissue engineering. [0003] Gelatin hydrogels for tissue engineering can be injected into the body in the form of cell / carrier complexes and cured in situ to repair defects, or can be cured in vitro and directly implanted in the body, and can Different drugs are added to the gel to achieve a specific slow-release effect. [0004] There are usually two ways to prepare g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/075C08L89/06C08L71/02C08J3/24A61L31/14A61L31/04A61L31/06A61L27/56A61L27/52A61L27/26A61L27/22A61L27/18A61L27/50A61K47/14A61K47/42A61K9/06C12N5/00
CPCA61K9/06A61K47/14A61K47/42A61L27/18A61L27/222A61L27/26A61L27/50A61L27/52A61L27/56A61L31/041A61L31/045A61L31/06A61L31/145A61L31/146A61L2400/06A61L2430/02C08J3/075C08J3/246C08J2389/06C08J2471/02C08L71/02C08L89/06C08L2201/08C08L2205/05C08L2312/00C12N5/00C12N2533/54
Inventor 王毅虎郭燕川
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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