Method for purifying Lycium barbarum polysaccharides

A technology of Lycium barbarum polysaccharides and components of Lycium barbarum polysaccharides, which can be used in anti-toxins, allergic diseases, drug combinations, etc., can solve the problems of difficulty in comprehensively evaluating the functions of Lycium barbarum polysaccharides, easy loss of active components, and complicated operations, and achieve strong free radicals. Cleansing and reducing power, enhanced phagocytosis, simple operation steps

Inactive Publication Date: 2017-09-15
NORTHWEST UNIV
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Problems solved by technology

[0005] The method for refining Lycium barbarum polysaccharides provided by the present invention solves the problems of complex operation, low yield, easy loss of active components and difficulty in comprehensively evaluating the functions of Lycium barbarum polysaccharides in the prior art method for separation and purification of Lycium barbarum polysaccharides

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  • Method for purifying Lycium barbarum polysaccharides
  • Method for purifying Lycium barbarum polysaccharides
  • Method for purifying Lycium barbarum polysaccharides

Examples

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Embodiment 1

[0042] A method for refining Lycium barbarum polysaccharide, comprising the following steps:

[0043] Step 1, extraction of Lycium barbarum polysaccharide

[0044] Step 1.1, take 500g of wolfberry, crush it with a tissue masher (Jintan Xinhang JJ-2 tissue masher homogenizer), soak it in 1500mL distilled water, adjust the pH to 7.0 with 1mol / L sodium hydroxide solution, Soak for 24h, centrifuge at 8000r / min for 10min, collect the first supernatant and the first precipitation; add 750mL distilled water to the first precipitation, soak for 6h, centrifuge at 8000r / min for 10min, collect the second supernatant; combine For the first supernatant and the second supernatant, the obtained mixed solution (about 2250mL) was placed below 40°C and concentrated to 400mL by rotary evaporation; then 1600mL of absolute ethanol was added to the concentrated solution, and after 12 hours of precipitation , centrifuge at 8000r / min for 10min, collect the precipitate;

[0045] Step 1.2, add 400mL ...

Embodiment 2

[0057] A method for refining Lycium barbarum polysaccharide, comprising the following steps:

[0058] Step 1, extraction of Lycium barbarum polysaccharide

[0059] With the step 1 of embodiment 1;

[0060] Step 2, the refining of macromolecular Lycium barbarum polysaccharide

[0061] Make the macromolecule Lycium barbarum polysaccharide into an aqueous solution with a concentration of 1g / 100mL (distilled water as the solvent), take 140mL, slowly add 60mL of absolute ethanol, so that the volume fraction of ethanol in the solution is 30%, precipitate for 12h, centrifuge at 8000r / min for 10min, collect The first polysaccharide supernatant and the first polysaccharide precipitation were reconstituted by adding 80 mL of distilled water to the first polysaccharide precipitation, centrifuged at 8000 r / min for 10 min, and the collected supernatant was freeze-dried at a temperature of -50 °C and a vacuum of 5-10Pa, the first Lycium barbarum polysaccharide fraction (LBP-I-1) was obtai...

Embodiment 3

[0069] A method for refining Lycium barbarum polysaccharide, comprising the following steps:

[0070] Step 1, extraction of Lycium barbarum polysaccharide

[0071] With the step 1 of embodiment 1;

[0072] Step 2, the refining of macromolecular Lycium barbarum polysaccharide

[0073] Make the macromolecule Lycium barbarum polysaccharide into an aqueous solution with a concentration of 1g / 100mL (the solvent is distilled water), take 35mL, slowly add 15mL of absolute ethanol, so that the volume fraction of ethanol in the solution is 30%, precipitate for 12h, centrifuge at 8000r / min for 10min, collect The first polysaccharide supernatant and the first polysaccharide precipitation were reconstituted by adding 30 mL of distilled water to the first polysaccharide precipitation, centrifuged at 8000 r / min for 10 min, and the collected supernatant was freeze-dried at a temperature of -50 °C and a vacuum of 5-10Pa, the first Lycium barbarum polysaccharide fraction (LBP-I-1) was obtain...

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Abstract

The invention belongs to the technical field of separation and purification of Lycium barbarum polysaccharides, and concretely discloses a method for purifying Lycium barbarum polysaccharides. The method comprises the following steps: carrying out water extraction, alcohol precipitation, dialysis and freeze drying to obtain crude Lycium barbarum polysaccharides, dialyzing the crude Lycium barbarum polysaccharides through a 8-14 KDa to obtain macromolecular and micro-molecualr Lycium barbarum polysaccharides, sequentially carrying out gradient precipitation on the macromolecular Lycium barbarum polysaccharides with an ethanol solution with the volume fraction of 30%, an ethanol solution with the volume fraction of 50% and an ethanol solution with the volume fraction of 70%, collecting precipitates at different gradients, freeze-drying the precipitates to obtain three primarily-purified Lycium barbarum polysaccharide components, and respectively separating and purifying the three primarily-purified Lycium barbarum polysaccharide components through Sephadex G-100 column chromatography to obtain three highly-pure Lycium barbarum polysaccharide components LBGP-I-1, LBGP-I-2 and LBGP-I-3. The monosaccharide composition of the LBGP-I-1 is based on glucose, the monosaccharide composition of the LBGP-I-2 is based on glucose and galactose, and the monosaccharide composition of the LBGP-I-3 is based on arabinose and galactose. The monosaccharide compositions of the LBGP-I-1 and the LBGP-I-2 are different from that of polysaccharides purified through traditional methods. The method disclosed in the invention has the advantages of simple operating steps, low cost and high yield, allows the Lycium barbarum polysaccharide components having different structural compositions to be obtained, and is suitable for large-scale industrial production of the Lycium barbarum polysaccharides.

Description

technical field [0001] The invention belongs to the technical field of separation and purification of wolfberry polysaccharide, and in particular relates to a method for refining wolfberry polysaccharide. Background technique [0002] Lycium barbarum belongs to the family Solanaceae and is mainly distributed in Northwest China. It has been used as a traditional Chinese medicine for more than 2,000 years. It is currently widely used as a functional food in East Asia, Europe, North America and China, with a large market share. Studies have shown that wolfberry is rich in a variety of active ingredients, including 18 kinds of amino acids (8 kinds of essential amino acids for the human body), 21 kinds of trace elements, flavonoids, proteins, polysaccharides, carotenoids, etc. [0003] Lycium barbarum polysaccharide is one of the most important active ingredients of Lycium barbarum. It has various biological activities such as lowering blood sugar, lowering blood fat, anti-oxidat...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06A61P37/04
CPCC08B37/0003C08B37/00C08B37/006
Inventor 王仲孚龚桂萍孙静黄琳娟
Owner NORTHWEST UNIV
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