Stabilizer used for nucleic acid of circulating tumor and cell in blood in liquid biopsy and blood collecting vessel
A liquid biopsy and stabilizer technology, which is applied in the preservation of human or animal body, the determination/inspection of microorganisms, biochemical equipment and methods, etc. Effect
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Embodiment 1
[0048] The present embodiment provides a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood. In parts by weight, the stabilizer includes 50 mg of preservative (graphene nano-antibacterial material), 25 mg of di-tert-butylhydroxytoluene, 10 mg Dipotassium diethylamine tetraacetate, 15 mg of mercaptoethanol, 2 mg of apoptosis inhibitor Q-VD-OPh, 20 mg of free radical scavenger and 10 mg of aurin tricarboxylic acid, by changing the pH stabilizer citric acid content, so that the final pH value of the stabilizer is between 4-5.
[0049] In the stabilizer of the present embodiment, the preparation method of the graphene nano-antibacterial material is: utilize hydrothermal method to cultivate graphene nano-material, and dissolve 1g of graphene nano-material in 50mL ultrapure water and ultrasonically disperse to obtain a dispersion ; Then add 20-50g of octadecyldimethyltrimethoxysilyl propyl ammonium chloride to the dispersion, ultrasonically shake for 48 h...
Embodiment 2
[0058] The present embodiment provides a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood, in parts by weight, the stabilizer includes 35 mg of graphene nano-antibacterial material 20 mg of tert-butyl hydroquinone, 35 mg of diethylamine Dipotassium tetraacetate, 10 mg of dithiothreitol, 5 mg of apoptosis inhibitor Q-VD-OPh, 25 mg of free radical scavenger and 5 mg of glucosamine hydrochloride, by changing the content of pH stabilizer citric acid , so that the final pH value of the stabilizer is between 4-5.
[0059] In the stabilizer of the present embodiment, the preparation method of the graphene nano-antibacterial material is: utilize the hydrothermal method to cultivate the graphene nano-material, and dissolve 1g of the graphene nano-material in 50mL ultrapure water and ultrasonically disperse it to obtain a dispersed liquid; then add 20-50g octadecyl dimethyl trimethoxysilyl propyl ammonium chloride to the dispersion, ultrasonically shake for...
Embodiment 3
[0068] The present embodiment provides a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood, in parts by weight, the stabilizer includes 25 mg of preservative (formed by rifampicin, protamine, chitosan and ethylparaben) The composition of composition, wherein: rifampicin, protamine, chitosan and ethylparaben mass ratio are 1:5:2:5), 25mg of 2,3,6,7,10,11-6 Hydroxytriphenyl, 25 mg of dipotassium diethylamine tetraacetate, 15 mg of sodium bisulfite, 10 mg of apoptosis inhibitor Q-VD-OPh, 25 mg of free radical scavenger and 5 mg of glucosamine hydrochloride, passed Change the content of the pH stabilizer citric acid so that the final pH value of the stabilizer is between 4-5.
[0069] In the stabilizer of the present embodiment, the preparation method of the free radical scavenger is:
[0070] Using ethylenediamine as an initiator, dissolve it in 50mL of dichloromethane solution, and use stannous octoate as a catalyst to initiate the polymerization ri...
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