Stabilizer used for nucleic acid of circulating tumor and cell in blood in liquid biopsy and blood collecting vessel

A liquid biopsy and stabilizer technology, which is applied in the preservation of human or animal body, the determination/inspection of microorganisms, biochemical equipment and methods, etc. Effect

Active Publication Date: 2017-09-15
云南仁桥医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problem that the existing blood sample preservation stabilizer is difficult to meet the integrity of free deoxyribonucleic acid (cfDNA), circulating tumor DNA (cfDNA) and wild-type genome in liquid biopsy and the harmful components such as formaldehyde are easily produced, the purpose of the present invention is to provide A stabilizer that does not contain harmful substances such as formaldehyde and can be used for the preservation of circulating tumor nucleic acid and cells in liquid biopsy blood and blood collection tubes thereof that meets medical standards

Method used

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  • Stabilizer used for nucleic acid of circulating tumor and cell in blood in liquid biopsy and blood collecting vessel
  • Stabilizer used for nucleic acid of circulating tumor and cell in blood in liquid biopsy and blood collecting vessel
  • Stabilizer used for nucleic acid of circulating tumor and cell in blood in liquid biopsy and blood collecting vessel

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Embodiment 1

[0048] The present embodiment provides a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood. In parts by weight, the stabilizer includes 50 mg of preservative (graphene nano-antibacterial material), 25 mg of di-tert-butylhydroxytoluene, 10 mg Dipotassium diethylamine tetraacetate, 15 mg of mercaptoethanol, 2 mg of apoptosis inhibitor Q-VD-OPh, 20 mg of free radical scavenger and 10 mg of aurin tricarboxylic acid, by changing the pH stabilizer citric acid content, so that the final pH value of the stabilizer is between 4-5.

[0049] In the stabilizer of the present embodiment, the preparation method of the graphene nano-antibacterial material is: utilize hydrothermal method to cultivate graphene nano-material, and dissolve 1g of graphene nano-material in 50mL ultrapure water and ultrasonically disperse to obtain a dispersion ; Then add 20-50g of octadecyldimethyltrimethoxysilyl propyl ammonium chloride to the dispersion, ultrasonically shake for 48 h...

Embodiment 2

[0058] The present embodiment provides a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood, in parts by weight, the stabilizer includes 35 mg of graphene nano-antibacterial material 20 mg of tert-butyl hydroquinone, 35 mg of diethylamine Dipotassium tetraacetate, 10 mg of dithiothreitol, 5 mg of apoptosis inhibitor Q-VD-OPh, 25 mg of free radical scavenger and 5 mg of glucosamine hydrochloride, by changing the content of pH stabilizer citric acid , so that the final pH value of the stabilizer is between 4-5.

[0059] In the stabilizer of the present embodiment, the preparation method of the graphene nano-antibacterial material is: utilize the hydrothermal method to cultivate the graphene nano-material, and dissolve 1g of the graphene nano-material in 50mL ultrapure water and ultrasonically disperse it to obtain a dispersed liquid; then add 20-50g octadecyl dimethyl trimethoxysilyl propyl ammonium chloride to the dispersion, ultrasonically shake for...

Embodiment 3

[0068] The present embodiment provides a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood, in parts by weight, the stabilizer includes 25 mg of preservative (formed by rifampicin, protamine, chitosan and ethylparaben) The composition of composition, wherein: rifampicin, protamine, chitosan and ethylparaben mass ratio are 1:5:2:5), 25mg of 2,3,6,7,10,11-6 Hydroxytriphenyl, 25 mg of dipotassium diethylamine tetraacetate, 15 mg of sodium bisulfite, 10 mg of apoptosis inhibitor Q-VD-OPh, 25 mg of free radical scavenger and 5 mg of glucosamine hydrochloride, passed Change the content of the pH stabilizer citric acid so that the final pH value of the stabilizer is between 4-5.

[0069] In the stabilizer of the present embodiment, the preparation method of the free radical scavenger is:

[0070] Using ethylenediamine as an initiator, dissolve it in 50mL of dichloromethane solution, and use stannous octoate as a catalyst to initiate the polymerization ri...

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Abstract

The invention provides a stabilizer used for a nucleic acid of a circulating tumor and a cell in blood in liquid biopsy and a blood collecting vessel. The stabilizer comprises the following materials by weight: 20-100 parts of a preservative, 5-50 parts of an antioxidant, 1-35 parts of an anticoagulant, 5-20 parts of a pH stabilizer, 5-20 parts of an enzyme inhibitor, 0.1-10 parts of an apoptosis inhibitor, 5-25 parts of a radical scavenger, and 1-10 parts of a metabolic inhibitor. According to the stabilizer used for the nucleic acid of the circulating tumor and the cell in blood in liquid biopsy and the blood collecting vessel, a related sample is rapidly separated according to a one-step method, the free nucleic acid of a fetus is effectively stored, the crosslinking damage of the nucleic acid of the circulating tumor is prevented, the integrity of the wild type gene is guaranteed, the fragmentation of the cell and the release of the genome nucleic acid of the blood are prevented, and the circulating tumor cell, the lymphocyte and the nucleic acid of the circulating tumor in a blood sample of a cancer patient are stored and transported at the normal temperature.

Description

technical field [0001] The invention belongs to the technical field of liquid biopsy, and in particular relates to a stabilizer for circulating tumor nucleic acid and cells in liquid biopsy blood and a blood collection tube thereof. Background technique [0002] Liquid biopsy not only has extremely high scientific research value, but also has great potential to help promote the clinical practice of precision medicine. It can be used in many fields such as early cancer screening, diagnosis, monitoring, treatment plan guidance and efficacy evaluation. The advantages of liquid biopsy make it the most promising means of tumor diagnosis and treatment. As a new type of medical diagnostic marker, free nucleic acid is closely related to the development of oncology and prenatal genetic diagnosis. Finally, it is called the landmark detection substance for early diagnosis of tumors, personalized treatment, and curative effect detection. The high heterogeneity of malignant tumors lead...

Claims

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Application Information

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IPC IPC(8): A01N1/02C12N15/10A61B5/154
CPCA01N1/021A01N1/0226A61B5/15003A61B5/150343A61B5/154C12N15/1003C12Q2527/125
Inventor 陈晓梅马圆
Owner 云南仁桥医疗科技有限公司
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