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DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry

A technology of blueberry and bacteria blocks, which is applied in the application field of agricultural ecological microorganisms, can solve the problems of slow growth, inconspicuous main root, and long growth cycle, and achieve the effects of reducing the degree of peroxidation, improving drought resistance, and removing active oxygen

Active Publication Date: 2017-09-05
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the blueberry large-scale cultivation technology has been relatively mature, there are still problems such as difficulty in rooting, slow growth, and long growth cycle in the process of blueberry seedling cultivation.
Moreover, because blueberry is a shallow-rooted plant, the root system is slender and underdeveloped, the tap root is not obvious, there is no root hair, and it cannot absorb the water in the deep layer of the soil, so blueberry is vulnerable to drought.

Method used

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  • DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry
  • DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry
  • DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The isolation and purification of blueberry root endophytic fungi are as follows:

[0025] 1. Wash the blueberry root section with clean water, and then transfer it to an ultra-clean workbench for the following operations: soak the root section in 10% (mass ratio) hydrogen peroxide for 8 minutes, and gently turn it several times with sterile tweezers to Ensure thorough disinfection; after rinsing with sterile water for 2-3 times, turn it into 6% (mass ratio) sodium hypochlorite, soak for 15 minutes, and rinse with sterile water for 2 times; filter paper absorbs the residual liquid on the surface of the root segment. The sterilized root segments were cut into 0.5 cm long root segments with sterile scissors, inserted into the prepared PDA plate, and cultured upside down in the dark in a constant temperature incubator at 25°C. Recipe for PDA plate: 0.6% potato dipping powder, 2% glucose, 2% agar, pH5.6. Sterilize at 115°C for 20 minutes, when cooled to 60°C, add sterile am...

Embodiment 2

[0028] Screening of growth-promoting beneficial mycorrhizal fungi, the specific steps are as follows:

[0029] 1. Cultivation of blueberry seedlings

[0030] (1) Differentiation of blueberry buds

[0031] Select blueberry tissue culture seedlings with good growth status and plant height of 4-5cm for rapid propagation. Use sterile scissors to cut off the blueberry plant in the tissue culture bottle from the base in an ultra-clean workbench, cut into 2-3cm stem segments, and then use sterile tweezers to connect it to the tissue culture bottle containing the differentiation medium , put 3-4 stem segments in each bottle, and spread them evenly. The medium used is based on WPM medium as the basic medium. In each liter of WPM medium, 20g of sucrose, 5-10g of agar, 1.0-3.0mg of phytohormone zeatin are added, and the pH of the medium is 5.0-5.5. The volume of the container is 10-30%, and it is autoclaved at 121°C for 20 minutes. The culture conditions are set at 23-25° C., light i...

Embodiment 3

[0043] Morphological and molecular biological identification of DSE bacterial strains, the steps are as follows:

[0044] 1. Pick the above-mentioned preserved purified strains, inoculate them on PDA plates, and cultivate and activate them in a constant temperature incubator at 25-30°C for 1-2 weeks. Use a sterile puncher with a diameter of 0.5 cm to take the bacteria cake from the outermost layer of the colony, inoculate it on a new PDA plate, and cultivate it in a constant temperature incubator at 25-30°C for 1 week to observe the shape of the colony. The colony is white, thin felt-like, flat, with smooth edges, and the opposite side of the colony is light yellow ( image 3 ).

[0045] 2. Use an inoculation needle to pick a small amount of purified single-colony hyphae, put it on a glass slide with a drop of normal saline to make a temporary mount, and observe the hyphae structure of the endophytic fungus under a microscope. The hyphae are septated, easy to gather into bun...

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Abstract

The invention discloses a DSE (Dark Septate Endophyte) bacterium and application thereof in promoting growth and preventing drought of blueberry, and belongs to the field of agricultural microorganisms. The invention provides the DSE bacterium R10, and the preservation number is CGMCC No.13882. When the DSE bacterium R10 is inoculated, the biomass of ground parts of blueberry, the photosynthetic rate of leaves, the protection enzyme activities such as SOD (Superoxide Dismutase) and POD (Peroxidase) are remarkably improved, and the contents of malondialdehyde and H2O2 in leaves are remarkably reduced, so that by adopting the strain, the accumulation of organic matters can be improved by increasing the photosynthetic rate of leave of host plants on one hand, on the other hand, reactive oxygen can be effectively eliminated by improving the activity of the protection enzymes, the peroxidation degree of drought degrees to membrane lipid is reduced, and the drought resistance of the blueberry is improved.

Description

technical field [0001] The invention belongs to the application field of agricultural ecological microorganisms, and specifically relates to a method for improving blueberry growth and drought resistance by using DSE fungi that have a close symbiotic relationship with plants to establish a mutually beneficial symbiotic relationship through co-cultivation. Background technique [0002] The symbiosis between plant roots and their rhizosphere microorganisms is a ubiquitous biological phenomenon in nature. Dark septate endophytes (Dark Septate Endophytes, DSE) are a kind of fungi that widely exist in plant roots and form dark septate hyphae. The ecological distribution of DSE is very wide, especially in various adversity ecological environments, and it plays an important role in the survival of host plants in various adversity ecosystems. Therefore, the study of DSE has become one of the focuses in the field of mycorrhizal research at home and abroad. [0003] Blueberry (Vacci...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00C12R1/645
CPCA01N63/30C12N1/14C12N1/145C12R2001/645
Inventor 王磊宿红艳李敏张文秀伍苏琼曹思琪李媛媛
Owner LUDONG UNIVERSITY
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