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Rapid analysis method of free amino acid content in fresh tea sample

A free amino acid, rapid analysis technology, applied in the field of rapid analysis of free amino acid content in fresh tea samples, can solve the problems of high cost, slow speed and low efficiency.

Inactive Publication Date: 2017-08-25
SUZHOU ACADEMY OF AGRI SCI
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Problems solved by technology

[0005] The present invention provides a rapid analysis method for free amino acid content in fresh tea samples, and its purpose is to solve the problems of slow speed, low efficiency, high cost, and difficulty in basic application and popularization existing in existing tea amino acid analysis methods

Method used

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  • Rapid analysis method of free amino acid content in fresh tea sample
  • Rapid analysis method of free amino acid content in fresh tea sample
  • Rapid analysis method of free amino acid content in fresh tea sample

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Embodiment

[0054] Example: A method for rapid analysis of free amino acid content in fresh tea samples

[0055] The rapid analysis method includes the following two parts:

[0056] The first part is to prepare a solution, and then use the high performance liquid chromatography-fluorescence detection method to establish a standard curve of the measured free amino acids with known gradient concentrations; the establishment of the standard curve consists of the following steps:

[0057] Step (1), prepare amino acid standards, namely aspartic acid, serine, glutamic acid, histidine, glycine, arginine, threonine, alanine, proline, theanine, cystine Amino acid, tyrosine, valine, methionine, lysine, isoleucine, leucine, phenylalanine;

[0058] Preparing instruments and equipment: 2695 high performance liquid chromatograph with 2475 fluorescence detector (Waters Company); TG16-WS desktop high-speed centrifuge (Hunan Xiangyi Experimental Instrument Company); K600 pulverizer (German Braun Company)...

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Abstract

Disclosed is a rapid analysis method of the free amino acid content in a fresh tea sample. The rapid analysis method is characterized by comprising the steps of mincing and uniformly mixing the fresh tea sample, using ultrapure water as an extraction solvent, conducting digestion under the condition of 90 DEG C for 20 min, after centrifugal filtration is conducted, using 6-aminoquinoline-N-hydroxysuccinimdyl carbamate as a derivating agent to conduct pre-column derivatization, using an XBridge C18 chromatographic column to conduct gradient elution, and using a high performance liquid phase-fluorescence detector to conduct separation and detection. According to the rapid analysis method of the free amino acid content in the fresh tea sample, sample preparation is quick and simple, the cost is low, the degree of precision and the degree of accuracy are high, and the stability and linear relation are good, the whole analysis process is quick, sensitive and good in reproducibility, and thus the rapid analysis method is applicable to quick analysis of free amino acid in the fresh tea sample.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a rapid analysis method for the content of free amino acids in fresh tea samples, which can simultaneously separate and analyze 18 kinds of free amino acids in fresh tea samples. Background technique [0002] Tea is one of the three major natural beverages in the world. It has unique aroma, rich nutrition and health benefits, and is deeply loved by consumers. Tea is rich in amino acids, which are important components that make up the taste of tea and directly affect the quality of tea. There are two main forms of amino acids in tea, one exists in free form; the other exists in peptides and proteins in combined form. Among them, free amino acids, such as theanine, glutamic acid, arginine, serine, etc., are a kind of important nitrogen-containing substances in tea. They are not only the basic unit of tea protein synthesis, but also the synthesis of many physiological and meta...

Claims

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Application Information

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IPC IPC(8): G01N30/74G01N30/02
CPCG01N30/02G01N30/74
Inventor 刘腾飞董明辉张丽杨代凤顾俊荣范君钱辉
Owner SUZHOU ACADEMY OF AGRI SCI
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