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Method for rapidly and accurately analyzing theanine in fresh tea leaves based on liquid chromatography-mass spectrometry

A liquid chromatography-mass spectrometry and theanine technology, which is applied to the analysis of materials, material separation, and measurement devices, can solve the problems of cumbersome steps, susceptibility to interference from impurities, low derivatization accuracy, and low detection accuracy, and achieve simplification Experimental procedures, elimination of false positive interference, accurate and reliable results of measurement data

Inactive Publication Date: 2021-12-31
SUZHOU VOCATIONAL UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventive method requires complex pre-column derivatization, and only relies on retention time for qualitative confirmation. There are problems such as cumbersome steps, easy to be interfered by impurities, and low derivatization accuracy and detection accuracy.

Method used

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  • Method for rapidly and accurately analyzing theanine in fresh tea leaves based on liquid chromatography-mass spectrometry
  • Method for rapidly and accurately analyzing theanine in fresh tea leaves based on liquid chromatography-mass spectrometry
  • Method for rapidly and accurately analyzing theanine in fresh tea leaves based on liquid chromatography-mass spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Fresh tea samples are taken from Suzhou Dongting Dongshan

[0059] Sample Preparation

[0060] Take fresh tea leaves, mix them evenly, weigh 0.1g, add 10mL ultrapure water, vortex mix, extract at 4°C for 30min, centrifuge at 9000r / min for 2min, take 4mL of the upper layer extract, add an equal amount of 80% ethanol The water (volume ratio) solution was centrifuged at 9000 r / min for 10 min at 4°C, and the supernatant was taken to pass through a 0.45 μm water phase filter membrane for testing.

[0061] The method established above was used for determination, and the results are shown in Table 4.

[0062] Table 4 Determination results of free amino acids in fresh leaves of Suzhou Dongting East tea

[0063]

Embodiment 2

[0065] Fresh tea samples are taken from Dongting Xishan, Suzhou

[0066] Sample Preparation

[0067] Take fresh tea leaves, mix them evenly, weigh 0.1g, add 10mL ultrapure water, vortex mix, extract at 4°C for 30min, centrifuge at 9000r / min for 2min, take 4mL of the upper layer extract, add an equal amount of 80% ethanol The water (volume ratio) solution was centrifuged at 9000 r / min for 10 min at 4°C, and the supernatant was taken to pass through a 0.45 μm water phase filter membrane for testing.

[0068] The method established above was used for determination, and the results are shown in Table 5.

[0069] Table 5 Determination results of free amino acids in fresh tea leaves

[0070]

[0071]

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Abstract

The invention provides a method for rapidly and accurately analyzing theanine in fresh tea leaves based on a liquid chromatography-mass spectrometry technology. The method comprises the following steps: (1) pretreating the fresh tea leaves; (2) preparing a theanine standard solution; (3) obtaining optimal ultra-high performance liquid chromatography conditions and mass spectrometry conditions; and (4) carrying out qualitative confirmation on the theanine in the fresh tea leaves by adopting an ultra-high performance liquid chromatography-mass spectrometry according to the chromatography / mass spectrometry conditions in the step (3), and carrying out quantitative analysis. According to the method, fresh tea leaves are used as a sample instead of a dry tea leaf sample, purified water is used for low-temperature extraction, a certain proportion of ethanol water is used for purification and impurity removal, and ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry is used for detection, so that the purpose of rapidly and accurately determining the theanine component in the fresh tea leaves is achieved; and an ideal analysis means is provided for tea quality evaluation and grade evaluation.

Description

technical field [0001] The invention belongs to the field of food detection and relates to a detection method for chemical components, in particular to a method for rapidly and accurately analyzing theanine in fresh tea leaves based on liquid mass spectrometry technology. Background technique [0002] The buds or leaves of the new shoots of tea trees are rich in various amino acids, whose composition and content are closely related to the quality of tea, and are one of the important evaluation factors of tea quality. At present, there are more than 20 kinds of tea amino acids that can be identified, among which theanine accounts for more than 40% of the total free amino acids in tea. The bitterness and astringency of tea can enhance its sweetness, and it is also the main source of fresh and refreshing taste of tea. Therefore, the analysis of theanine in tea is of great significance for the quality identification of tea. [0003] Fresh tea leaves are fresh buds or leaves pi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72G01N30/86
CPCG01N30/02G01N30/06G01N30/72G01N30/8634G01N2030/047
Inventor 张丽康法刘腾飞张季雨
Owner SUZHOU VOCATIONAL UNIV
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