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Method for detecting benzoic acid in drink

A benzoic acid and beverage technology, applied in the field of food analysis, to achieve the effects of increased hydrophilicity, fast and efficient detection, and simple operation

Inactive Publication Date: 2017-08-18
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no method for the detection of benzoic acid using thin film microextraction combined with surface-enhanced Raman spectroscopy.

Method used

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  • Method for detecting benzoic acid in drink
  • Method for detecting benzoic acid in drink
  • Method for detecting benzoic acid in drink

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0034] A method (TFME-SERS method) for detecting benzoic acid in beverages, comprising:

[0035] 1) Preparation of silica gel extraction substrate:

[0036] 1-1) Carry out hydroxylation treatment on the substrate of the glass sheet: ultrasonically clean the glass sheet with a size of 1.0cm×1.0cm in ultrapure water, ethanol, and acetone for 10 minutes respectively, and blow dry with nitrogen; add 35mL of Concentrated sulfuric acid, add 15mL of hydrogen peroxide to it while stirring, after the mixture is cooled, add the glass flakes that have been washed and blown dry with nitrogen into the mixture of concentrated sulfuric acid and hydrogen peroxide, heat in a water bath at 90°C for 1 hour, take out the glass flakes, and Rinse with pure water, blow dry with nitrogen;

[0037] 1-2) Add 0.12 g of sodium carboxymethyl cellulose to 30 mL of ultrapure water at 60°C, stir to dissolve, and obtain a sodium carboxymethyl cellulose solution with a concentration of 0.4%;

[0038] 1-3) Po...

experiment example 1

[0047] This experimental example characterizes the silica gel extraction substrate obtained in the example. figure 1 a is a scanning electron microscope image of the silica gel film extraction material in the silica gel layer on the silica gel extraction substrate. The figure shows that the microscopic surface of the silica gel material is in a rough and amorphous state with many holes, which makes the coating have more adsorption sites and Larger adsorption area. Such as figure 1 As shown in b, from the cross-sectional SEM image of the silica gel extraction substrate, it can be known that the thickness of the silica gel extraction coating is about 130 μm, and a thinner coating thickness is conducive to the rapid mass transfer process, and a larger extraction area ensures a larger The adsorption capacity helps to improve the sensitivity of the analytical method.

experiment example 2

[0049] In this experimental example, the reproducibility of the detection method in the embodiment was tested, and the reproducibility of the SERS signal at different sampling points on the same silica gel extraction substrate and the reproducibility of the same analyte signal from different silica gel extraction substrates were investigated. Firstly, the experiment used 500 μg / mL standard solution of benzoic acid as the extraction solution, and selected a single piece of silica gel extraction substrate for TFME process, and then randomly selected 10 area points within the range where the extraction piece was sprayed with gold colloidal nanoparticles to collect its SERS signal. The result is as figure 2 shown. It can be seen that the SERS signal of the analyte benzoic acid obtained in different regions of the silica gel extraction substrate is very obvious and has a high consistency.

[0050] Secondly, the experiment randomly selected 5 different silica gel extraction substr...

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Abstract

The invention discloses a method for detecting benzoic acid in drink. The method comprises the steps of enriching benzoic acid in the drink through a silica gel extraction substrate, and detecting the benzoic acid in combination with surface-enhanced Raman spectroscopy. By combining the characteristics of high extraction efficiency, short extraction time and selective extraction of a target from a complicated substrate of a thin film microextraction technology with the advantages of convenience and quickness in detection and high sensitivity of the surface-enhanced Raman spectroscopy, a measuring method for the benzoic acid in the drink, which is easy to operate, high in sensitivity, low in detection cost, convenient and quick, is provided.

Description

technical field [0001] The invention belongs to the field of food analysis research, and in particular relates to a method for detecting benzoic acid in beverages, in particular to a method for detecting benzoic acid in beverages combined with thin film microextraction and surface-enhanced Raman spectroscopy. Background technique [0002] Benzoic acid is an acid preservative, and the undissociated acid has antimicrobial activity. Benzoic acid can be used as a food and drug preservative, which can effectively prevent food and drug from deteriorating and prolong the shelf life. Because benzoic acid has the strongest antibacterial activity in the liquid environment of pH 2.5-4.0, it is widely used as a preservative for beverages. Studies have shown that excessive intake of benzoic acid may cause symptoms such as diarrhea, abdominal pain and rapid heartbeat. Excessive benzoic acid can also interfere with the intermediate metabolic processes of the human body, including urea cy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/40G01N1/34
Inventor 王翊如蔡乐梅陈曦
Owner XIAMEN UNIV
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