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Oil-in-water vaccine adjuvant and preparation method thereof

A vaccine adjuvant, oil-in-water technology, applied in the field of vaccines, can solve problems such as poor stability of oil-in-water vaccine adjuvants, and achieve the effects of improving protection against virus, long stable period, and obvious immune effect.

Inactive Publication Date: 2017-08-18
天津三江永利生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defects of the prior art, and provides an oil-in-water vaccine adjuvant and its preparation method to solve the technical problem of poor stability of the oil-in-water vaccine adjuvant in the prior art

Method used

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  • Oil-in-water vaccine adjuvant and preparation method thereof
  • Oil-in-water vaccine adjuvant and preparation method thereof
  • Oil-in-water vaccine adjuvant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] An oil-in-water vaccine adjuvant, consisting of the following components: 2.5g lauryl glucoside, 2.3g polyvinylpyrrolidone, 1.1g glyceryl monostearate, 2.1g polyethylene glycol dioleate, white oil VACCUTE-L 89g, propylene glycol fatty acid ester 1.5g, polydextrose 1.0g Tween 80 0.5g.

[0040] Select 10 male and female SPF-grade Balb / c mice weighing 18-22 g and divide them into three groups. The vaccine adjuvant prepared above is selected as the experimental group, and the normal saline group is the blank control group. The oil-in-water vaccine adjuvant was used as the standard control group, and each dose was injected intramuscularly with 0.5 mL according to body weight conversion, and observed continuously for 2 weeks. Determination of body weight change, analysis of poisoning symptoms, and adverse reactions were carried out respectively. Experimental results such as figure 1 As shown, the body weight growth curve of the mice in the experimental group is highly consi...

Embodiment 2

[0046] An oil-in-water vaccine adjuvant, consisting of the following components: 3.5g polyvinylpyrrolidone, 1.3g guar gum, 2.0g fatty acid methyl ester, 1.2g polyethylene glycol dioleate, 88g white oil VACCUTE-L , D-Mannitol 1.5g, Propylene Glycol Alginate 1.5g, Span 80 1g.

Embodiment 3

[0048] An oil-in-water vaccine adjuvant, consisting of the following components: 1.93g lauryl glucoside, 2.45g guar gum, 1.7g glycerol monostearate, 1.22g fatty acid methyl ester, 92g white oil VACCUTE-L, Propylene glycol fatty acid ester 0.1 g, D-mannitol 0.1 g, Tween 80 0.5% (w / w).

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Abstract

The invention provides an oil-in-water vaccine adjuvant and a preparation method thereof. According to the technical scheme, category and composition of surfactant are optimized; the stable oil-in-water adjuvant is prepared by virtue of a one-step process, so that autologous cellular and humoral immunity stimulation capacities of a vaccine are enhanced; and especially, the vaccine adjuvant also simultaneously comprises other water-based adjuvant ingredients which are effective on enhancing efficacy of the vaccine, so that attacking protection efficacy of the inactivated vaccine is further improved, and technical problems of an existing oil-in-water vaccine which is low in immunological activity and short in immunity persistence are solved. The preparation method of the nano vaccine adjuvant provided by the invention is simple in process and low in cost, and the obtained vaccine adjuvant is stable in dosage form. The prepared oil-in-water nano emulsion (the vaccine adjuvant) is long in stable period, easy for preservation, good in immunization effect, long in protection term, easy for injection and quite low in side reactions.

Description

technical field [0001] The invention relates to the technical field of vaccines, in particular to an oil-in-water vaccine adjuvant and a preparation method thereof. Background technique [0002] Vaccines are preventive biological products that are immunogenic but do not cause disease in the body. The antigenic substances of vaccines are mostly taken from the inactivated or attenuated products of pathogenic microorganisms. When the inactivated antigen is immunized with animals alone, it is not enough to produce immunogenicity, especially the antigen with low immunogenicity and small molecular weight. Therefore, it is necessary to use different types of adjuvants to enhance the body's immune response to antigens. [0003] Adjuvants, also known as non-specific immunopotentiators, are not antigenic in themselves, and are a type of adjuvants that act prior to or simultaneously with antigens, and can non-specifically change or enhance immunogenicity or change the type of immune r...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/12A61P37/04A61P31/14
CPCA61K39/12A61K39/39A61K2039/5252A61K2039/552A61K2039/55511A61K2039/55583A61K2039/57A61K2039/575C12N2770/10034A61K2300/00
Inventor 米娜张忠江刘恒权孟莎莎刘金秋张心原
Owner 天津三江永利生物科技有限公司
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