Modified seasonal influenza-rsv combination vaccine and preparation method thereof

Abstract

The invention discloses a modified seasonal flu-RSV (Respiratory Syncytial Virus) combined vaccine and a preparation method thereof. The modified seasonal flu-RSV combined vaccine is prepared from a flu protein antigen, an RSV protein antigen and a nanoparticle carrier, wherein the surface of the nanoparticle carrier is hydroxylated; the flu protein antigen and the RSV protein antigen react with hydroxyl groups on the surface of the nanoparticle carrier and are connected to the surfaces to nanoparticles. According to the modified seasonal flu-RSV combined vaccine disclosed by the invention, the nanoparticles are used as a novel protein carrier, antigen protein is efficiently and stably connected, and the nanoparticles having a flu antigen and an RSV antigen at the same time are successfully constructed; an experiment on mice verifies that a good systemic immune response can be caused, the nanoparticles are used as the carrier and can be repeatedly synthesized, the stability is high, not only can digestion of all kinds of enzymes in a body be resisted, but also the modified seasonal flu-RSV combined vaccine has many advantages of tolerating high pressure, sterilizing and the like. By adopting the nanoparticles as the novel protein carrier, the method is simple, the operation is simple and convenient, various protein and polysaccharide antigens can be connected, too much modification is not needed, and a good application prospect is obtained.

Description

technical field [0001] The invention relates to a modified seasonal influenza-RSV combined vaccine and a preparation method thereof, belonging to the field of biotechnology. Background technique [0002] 1. Pathogenic microorganisms and vaccines [0003] Microorganisms that can cause infectious diseases in humans or animals are called pathogenic microorganisms or pathogenic microorganisms. Infection refers to the process of pathogenic microorganisms invading the body, growing and multiplying in certain parts, and causing a series of pathophysiological processes. When pathogenic microorganisms invade the body, the pathogenic microorganisms interact with the body and change each other's activity and function. Therefore, whether they can cause infectious diseases depends on the pathogenicity or virulence of the pathogenic microorganisms on the one hand, and on the other hand. Depends on the body's resistance, that is, immunity. The ability of pathogenic bacteria to cause inf...

AI Technical Summary

Problems solved by technology

In 2009, the Sandbulte MR research team used virus-like particles (VLP) containing only influenza virus NA for the detection of anti-NA antibodies. This method has applied for a patent. Due to the limited yield of the prepared VLP, there may also be baculovirus in the system. It is difficult for laboratories or vaccine manufacturers who lack the knowledge of VLP technology to carry out

The research of RSV vaccine has always been the focus of attention of the international community. From the existing RSV vaccines under development, it can be seen that injection immunization cannot produce effective mucosal and cellular immune responses and the immune protection effect is limited. DNA vaccines have potential safety and full-length Bottlenecks such as potential Th1 / Th2 imbalance in F and G protein vaccines need to be resolved urgently