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Lysine bacillus for preventing and controlling kiwi berry canker and application thereof

A technology of Bacillus lysinus and kiwifruit canker, which is applied in the field of microbiology, can solve the problems affecting the field control effect of canker and the drug resistance of canker bacteria, and achieve the effects of stable properties, strong inhibitory effect, and simple separation and screening methods

Inactive Publication Date: 2017-08-11
四川省自然资源科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extensive use of copper preparations has caused the resistance of canker bacteria, and the food safety problems caused by the extensive use of antibiotics have seriously affected the field control effect of canker

Method used

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  • Lysine bacillus for preventing and controlling kiwi berry canker and application thereof
  • Lysine bacillus for preventing and controlling kiwi berry canker and application thereof
  • Lysine bacillus for preventing and controlling kiwi berry canker and application thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Example 1 Isolation and screening of Bacillus lysinica CCTCC M2016557

[0027] The bacterial source of Bacillus lysinus CCTCC M2016557 of the present invention comes from Hongbai Town, Shifang City, Sichuan Province (31.36° north latitude, 104.03° west longitude, 861 meters above sea level), and the sampling time was May 2015.

[0028] The separation method is: 1. Rinse the collected kiwi branches with clear water, disinfect the surface with 75% alcohol, air-dry at room temperature, cut an appropriate amount of sample with a sterilized blade, put it into a grinding bag, add an appropriate amount of normal saline, grind, After soaking for 3 minutes, take 100 μL of the supernatant of the plant grinding liquid and add it to the newly prepared KB medium, and use an inoculation loop to coat and streak.

[0029] The purification method is as follows: After cultivating the sample petri dish at room temperature for a certain period of time, wait for the colonies to grow in the ...

Embodiment 2

[0030] The identification of embodiment 2 lysinibacillus CCTCC M2016557

[0031] 1. Observation of culture status

[0032] Activate the preserved bacteria on KB solid medium, culture at room temperature, observe the growth of the colony, and record the characteristics of the colony, including color, growth speed, shape, texture, etc.;

[0033] It is finally found that: the Bacillus lysinus CCTCC M2016557 bacterial strain of the present invention is milky white, colloidal, with a smooth and moist surface, raised center, neat edges, and normal viscosity; its bacterial species morphology is shown in figure 1 shown; its scanning electron microscope picture is shown in the sequence table.

[0034] 2. Molecular biological identification

[0035] DNA extraction: use bacterial PCR universal primer 27F (primer sequence is:

[0036] Eubac27F[106]5'-AGAGTTTGATCMTGGCTCAG-3') and 1492R (primer sequences are:

[0037] Eubac1492R[106]5'-TACGGYTACCTTGTTACGACT-3') amplified the 16S rDNA of...

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Abstract

The invention relates to the field of microbes, in particular to a lysine bacillus for preventing and controlling kiwi berry canker and application thereof. The preservation number of the lysine bacillus is CCTCC NO: M2016557. Pseudomonas is obtained from collected kiwi berry branches through separation and purification, a separating and screening method is simple, and the strain has the very strong inhibiting effect on pathogenic bacteria of bacterial kiwi berry canker. The strain SPANRS-213 is separated from healthy plant tissue, produced active metabolite has no obvious toxic effect on the plant tissue, the nature is stable, preparation is simple, spraying is convenient, the effect of preventing and controlling the kiwi berry canker is remarkable, an environment-friendly, simple and effective way is provided for prevention and control of the kiwi berry canker, and environmental protection is achieved.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a lysine bacillus for preventing and treating kiwi fruit canker and its application. Background technique [0002] Kiwifruit canker is a devastating bacterial disease. The disease mainly harms the trunk, branches, shoots and leaves of kiwifruit, and can easily cause plant death. The disease was discovered for the first time in Japan in 1980, and it was discovered successively in the United States after 1983. In 1987, the pathogen was identified as Pseudomonas syringaepv.actinidiae, the pathogenic variant of Pseudomonas syringa kiwifruit. Before 1984, there was no kiwifruit bacterial canker in my country. It was first discovered in Dongshanfeng Farm, Hunan Province in 1985, and spread rapidly to most kiwifruit cultivation areas in my country in just 10 years. The disease is ferocious and often breaks out on a large scale, resulting in large-scale destruction of gardens, ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P1/00C12R1/01
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/01
Inventor 庄启国李明章何利钦王丽华王永志张茜
Owner 四川省自然资源科学研究院
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