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Green algae polysaccharide and preparation method thereof

A technology of chlorophyll polysaccharides and sulfated polysaccharides, applied in the field of marine biological polysaccharides, can solve the problems of chlorophyll polysaccharide structure complexity, inhomogeneity, and restrictions on in-depth research, and achieve obvious anticoagulant activity, no toxic side effects, and good market application foreground effect

Active Publication Date: 2017-08-04
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complexity and heterogeneity of the structure of green algae polysaccharides, it greatly limits the in-depth research on it
So far, there is no report about the extraction and separation of sulfated polysaccharides from green algae, which are composed of glucuronic acid and rhamnose, and glucuronic acid is located in the branched chain.

Method used

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  • Green algae polysaccharide and preparation method thereof
  • Green algae polysaccharide and preparation method thereof
  • Green algae polysaccharide and preparation method thereof

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preparation example Construction

[0053] The present invention also provides a preparation method of Chlorella polysaccharide, said method comprising the steps of:

[0054] (1) adding water to the dry bag reef film to form a homogenate, extracting at 20°C-24°C for 0.5-4 hours, and separating the clear liquid; wherein, the quality of the water is 10-60 times that of the bag reef film;

[0055] (2) concentrating and desalting the obtained clear liquid under reduced pressure to obtain a first polysaccharide solution, concentrating and drying the first polysaccharide solution under reduced pressure to obtain a polysaccharide, and dissolving the polysaccharide in water to obtain a second polysaccharide solution, the The mass fraction of the polysaccharide in the second polysaccharide solution is 0.1%-6%;

[0056] (3) The second polysaccharide solution is separated by ion-exchange chromatographic column and gel chromatographic column, then concentrated under reduced pressure and desalted, and the desalted solution i...

Embodiment 1

[0063] (1) Add 1500 grams of water to 50 grams of dry bag reef film to soak to form a homogenate, stir and extract at 21°C for 3.0 hours, centrifuge to discard the precipitate, and separate the supernatant;

[0064] (2) concentrating the resulting clear liquid under reduced pressure, and dialysis and desalination to obtain the first polysaccharide solution. The step of dialysis and desalination includes: placing the clear liquid concentrated under reduced pressure in a dialysis bag with a molecular weight cut-off of 3500 Daltons for dialysis for 3 days , stop dialysis when the conductivity meter tests the external fluid to a constant value after dialysis;

[0065] Concentrate the first polysaccharide solution under reduced pressure, add ethanol with a mass fraction of 95% to the concentrated first polysaccharide solution to precipitate the polysaccharide, the volume of ethanol is four times the volume of the first polysaccharide solution, and place the resulting precipitate at ...

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Abstract

The invention discloses a green algae polysaccharide and a preparation method thereof. The green algae polysaccharide takes alpha-L-(1,3)-connection rhamnose and alpha-L-(1,2)-connection rhamnose as a main chain, and beta-D-glucuronic acid is positioned to a marine sulfated polysaccharide with a novel structure of a branch chain. The green algae polysaccharide has obvious anticoagulation, fibrinolysis, thrombolysis and blood sugar and blood lipid reducing activities and can be used for preparing anticoagulation and blood sugar reducing medicines and health food. The green algae polysaccharide is derived from natural seaweeds, does not have toxic and side effects on human bodies, can prepare tablets, powder, granules, capsules and the like serving as anticoagulation medicines for preventing and treating thromboembolic diseases and blood sugar reducing medicines for preventing and treating diabetes mellitus by adding various attached agents, and has good market application prospect.

Description

technical field [0001] The invention relates to the technical field of marine biological polysaccharides, in particular to a green algae polysaccharide and a preparation method thereof. Background technique [0002] Seaweed has been directly used as medicinal material to treat various diseases in the coastal folk of our country since ancient times. The research on the separation of active ingredients in seaweed and its chemical composition began in the mid-1950s. So far, among the various compounds isolated from seaweed, polysaccharides play an important role. Polysaccharides, as the main support material of algae cell structure, exist in large quantities in its cell wall and intercellular substance. Due to the unique structure and properties of seaweed polysaccharides, it has attracted widespread attention. The chemical composition, structure and biological activity of seaweed polysaccharides have been extensively studied. However, these studies have mainly focused on po...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P3/06A61P3/10A61P7/02A23L33/125
CPCA23V2002/00C08B37/0003A23V2200/30A23V2200/3262A23V2200/328
Inventor 毛文君刘雪
Owner OCEAN UNIV OF CHINA
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