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A Capsulated Rhodobacter and Its Application

A technology of capsular rhodobacteria and water bodies, applied in bacteria, biological water/sewage treatment, microorganisms, etc., can solve the problem of less photosynthetic bacteria of benzene series and formaldehyde, and achieve a wide range of effects

Active Publication Date: 2019-12-03
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are few reports on photosynthetic bacteria capable of degrading benzene series and formaldehyde at home and abroad

Method used

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  • A Capsulated Rhodobacter and Its Application
  • A Capsulated Rhodobacter and Its Application
  • A Capsulated Rhodobacter and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Isolation, purification and identification of Rhodobacter capsularis DC-1

[0036] 1. After removing impurities from the sewage collected from the entrance of Dianchi Wetland Park in Kunming, pour it into 500mL enrichment culture solution (formula NaCl 2.5 g, MgSO 4 ·7H 2 O 0.05 g, NH4Cl 1.0 g, NaHCO 3 2.5 g, KH 2 PO 4 2.75 g, CH 3 COONa 6 g, yeast powder 2 g, distilled water 1000 mL, pH value 7.0) in a mineral water bottle, mix well, seal; finally place at 28 ℃, 60W incandescent light culture for 7-8 days; Red or blood red, suck out 50mL of red liquid, pour it into a mineral water bottle filled with fresh enriched culture medium, and enrich and cultivate continuously for 3 times; use RCVBN culture medium (the formula is CH 3 COONa 5.0g, CH 3 CH 2 COONa 3.0g, NaCl 2.5g, MgSO 4 ·7H 2 O 1.5g, (NH 4 ) 2 SO 4 0.5g, KH 2 PO 4 1.5g, K 2 HPO 4 0.6g, CaCl 2 0.05g, MnSO 4 5mg, FeSO 4 5mg, yeast extract 0.5g, peptone 20mg, glutamic acid 0.5mg...

Embodiment 2

[0055] Embodiment 2: the optimization of photosynthetic bacteria culture condition

[0056] 1. Determination of temperature Add 20% seed liquid into a 25mL anaerobic tube, and use liquid medium (the formula is CH 3 CH 2 COONa 5g / L, (NH 4 ) 2 SO 4 2.0g / L, MgSO 4 •7H 2 O 0.2g / L, NaH 2 PO 4 •H 2 O 0.5g / L, K 2 HPO 4 0.66 g / L, CaCl 2 • 2H 2 O 0.1 g / L. ) was filled with anaerobic tubes, and under the condition of pH 7, the culture temperature was set to 20, 25, 30, and 35°C respectively; the light culture was 7 days, and the experiment was repeated 3 times, and the bacterial culture solution was measured every 24 hours The OD value at 375nm was used to determine the optimal culture temperature. Figure 6 is the effect of temperature on the growth of strain DC-1, by Figure 6 It can be seen that the optimal culture temperature of strain DC-1 is 30°C.

[0057] 2. Determination of pH Add 20% seed liquid into a 25mL anaerobic tube, and use a liquid medium (the formula i...

Embodiment 3

[0061] Embodiment 3: the ability of bacterial strain DC-1 to purify benzene series

[0062] The liquid culture of the strain DC-1 with a concentration of 20% was inoculated in the liquid medium containing 2mM, 4mM, and 6mM benzene respectively, and cultured at 30°C under the light of 60W incandescent lamp for 0, 1, 2, 3, 4, 5, 6 , 7, and 8 days, the experiment was set for 3 repetitions, and the cell concentration and residual liquid benzene concentration of strain DC-1 were measured at each time point; the specific steps for the determination of benzene content were as follows:

[0063] 1. Preparation of formaldehyde-sulfuric acid solution: add 0.10 mL of 10% formaldehyde solution to 100 mL of high-grade concentrated sulfuric acid, mix, store in ice cold (prepared when used);

[0064] (In the presence of sulfuric acid, benzene series reacts with formaldehyde to form a yellow-brown diphenylmethane polymer. The maximum absorption wavelength of this colored compound is 465 nm. Us...

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Abstract

The invention discloses a strain of capsulated rhodobacter ( Rhodobacter capsulatus strain) DC‑1, and its preservation number in the China Type Culture Collection Center is CCTCC NO: M2016676, the strain of the present invention is a photosynthetic bacterium that can efficiently degrade benzene series and formaldehyde, and can be used to treat sewage polluted by benzene series and formaldehyde , high tolerance concentration of benzene series, strong degradation effect; ability to remove formaldehyde, and the culture condition of this strain is simple, easy for industrial implementation.

Description

technical field [0001] The invention relates to a photosynthetic bacterium capsular rhodobacter and its application in the purification of formaldehyde and benzene series, belonging to the field of sewage treatment. Background technique [0002] Benzene series are common pollutants in the environment. They are carcinogenic, teratogenic, and mutagenic. They are listed as monitoring items in the national water quality standards for drinking water. Long-term exposure to benzene may cause chronic poisoning, leading to aplastic anemia and leukemia. Its main pollution comes from waste gas discharged from industries such as decoration materials and paints, and it is used as a disinfectant in the waste water produced by medical and sanitation and aquatic industries. Formaldehyde (HCHO), also known as "formaldehyde", is a colorless, easily soluble, slightly irritating gas with high biological activity and potential carcinogenicity at room temperature. Concealment features. The har...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/30C02F101/34
CPCC02F2101/30C02F2101/34C12N1/205C12R2001/01
Inventor 李昆志雷玉珠操璟璟包燚陈丽梅徐慧妮
Owner KUNMING UNIV OF SCI & TECH
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