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Yeast culture preparation method

A yeast culture and yeast technology, which is applied in the dissolution, fermentation, animal feed and other directions of microorganisms, can solve the problems of low content of yeast extracellular metabolites, difficult product quality control, low yeast content, etc. High, easy to control product process and quality, low equipment investment

Inactive Publication Date: 2017-07-28
高唐华农生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is also a process that combines solid-state fermentation and liquid-state fermentation, but there are defects such as low yeast content, low content of beneficial yeast extracellular metabolites, high content of miscellaneous bacteria, and difficult control of product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. A method for preparing yeast culture, comprising the following steps.

[0049] Step 1: Strain preparation and activation

[0050] Make a suspension of the purchased yeast strain, add sterile water to dilute to a concentration of 10 -8 At the same time, it is separated by coating or streaking on the solid plate medium, and some single colonies are picked to measure the production capacity; after repeated screening, it is determined that the yeast produced by fermentation has strong activity and a high number of viable bacteria. The strain replaces the original strain.

[0051] Step 2: Production of Inclined Strains

[0052] Configure the slant medium according to the recipe requirements, and the weight parts of each component of the slant medium are: 30 g of glucose, 6 g of peptone, 3 g of yeast extract, 20 g of agar powder, and dilute to 1000 mL with water.

[0053] Heat the slant culture medium to fully dissolve each component, then divide into several t...

Embodiment 2

[0069] Example 2. A method for preparing yeast culture, comprising the following steps.

[0070] Step 1: Strain preparation and activation

[0071] Make a suspension of the purchased yeast strain, add sterile water to dilute to a concentration of 10 -8 At the same time, it is separated by coating or streaking on the solid plate medium, and some single colonies are picked to measure the production capacity; after repeated screening, it is determined that the yeast produced by fermentation has strong activity and a high number of viable bacteria. The strain replaces the original strain.

[0072] Step 2: Production of Inclined Strains

[0073] Configure the slant medium according to the recipe requirements, and the weight parts of each component of the slant medium are: glucose 28g, peptone 5g, yeast extract 2g, agar powder 20g, add water to 1000mL.

[0074] Heat the slant culture medium to fully dissolve each component, then divide it into several test tubes, and seal the te...

Embodiment 3

[0090] Example 3. A method for preparing yeast culture, comprising the following steps.

[0091] Step 1: Strain preparation and activation

[0092] Make a suspension of the purchased yeast strain, add sterile water to dilute to a concentration of 10 -8 At the same time, it is separated by coating or streaking on the solid plate medium, and some single colonies are picked to measure the production capacity; after repeated screening, it is determined that the yeast produced by fermentation has strong activity and a high number of viable bacteria. The strain replaces the original strain.

[0093] Step 2: Production of Inclined Strains

[0094] Configure the slant medium according to the recipe requirements. The weight parts of each component of the slant medium are: 35 g of glucose, 7 g of peptone, 4 g of yeast extract, 25 g of agar powder, and dilute to 1000 mL with water.

[0095] Heat the slant culture medium to fully dissolve each component, then divide it into several te...

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Abstract

The invention discloses a yeast culture preparation method. The yeast culture preparation method includes strain preparation and activation, slant strain manufacturing, triangular flask liquid strain manufacturing, seed tank liquid strain manufacturing, solid fermentation, in-bag cell anaerobic autolysis, low-temperature drying and grinding to obtain a finished product. The yeast culture preparation method has unique advantages of water saving, energy saving, high saccharomycete quantity and environmental friendliness in production process and is further low in production cost.

Description

technical field [0001] The invention relates to the field of feed additives, in particular to a preparation method of yeast culture. Background technique [0002] Yeast are unicellular fungi that are not a unit of phylogenetic classification. It is a general term for several single-celled fungi such as Ascomycetes and Basidiomycetes. It generally refers to various single-celled fungi that can ferment sugars. It can be used in brewing production. Some are pathogenic bacteria. It is a model for genetic engineering and cell cycle research. biology. Yeast is the earliest microorganism used in the history of human civilization. Can survive in hypoxic environment. There are currently more than 1,000 species of yeast known. According to the ability of yeast to produce spores (ascospores and basidiospores), yeast can be divided into three categories: spore-forming strains belong to ascomycetes and basidiomycetes. Fungi that do not form spores but reproduce mainly by budding are ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12P1/02C12N1/06A23K10/18
CPCC12N1/06C12N1/16C12P1/02
Inventor 郝志刚
Owner 高唐华农生物工程有限公司
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